Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II
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Earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. We now demonstrate the feasibility of same in a crop like rice through overproduction of glyoxalase II. The rice glyoxalase II was cloned in pCAMBIA1304 and transformed into rice (Oryza sativa cv PB1) via Agrobacterium. The transgenic plants showed higher constitutive activity of glyoxalase II that increased further upon salt stress, reflecting the upregulation of endogenous glyoxalase II. The transgenic rice showed higher tolerance to toxic concentrations of methylglyoxal (MG) and NaCl. Compared with non-transgenics, transgenic plants at the T1 generation exhibited sustained growth and more favorable ion balance under salt stress conditions.
KeywordsFunctional validation Glyoxalase II overexpression Methylglyoxal Rice transgenics Salinity tolerance
We thank Professor Ray Wu, Cornell University, USA for valuable suggestions and critical reading of the manuscript. Thanks are also due to Drs. F White and BW Porter, Kansas State University, USA for the initial glyoxalase II clone and Drs. V. Rajamani and J. K. Tripathi, JNU, New Delhi for extending help in the work related to ionic content measurements. The financial support by the Department of Biotechnology (DBT, New Delhi) Rice Network Project, DBT Post-Doc fellowship to SKY and grants from the International Centre for Genetic Engineering and Biotechnology is duly acknowledged.
- Kumar S, Singla-Pareek SL, Reddy MK, Sopory SK (2003) Glutathione: biosynthesis, homeostasis and its role in abiotic stresses. J Plant Biol 30:179–187Google Scholar
- Ramaswamy O, Guha-Mukherjee S, Sopory SK (1983) Presence of glyoxalase I in pea. Biochem Inter 7:307–318Google Scholar
- Talesa V, Rosi G, Contenti S, Mangiabene C, Lupattelli M, Norton SJ, Giovannini E, Principato GB (1990) Presence of glyoxalase II in mitochondria from spinach leaves: comparison with the enzyme from cytosol. Biochem Inter 22:1115–1120Google Scholar
- Yadav SK, Singla-Pareek SL, Reddy MK, Sopory SK (2005b) Methylglyoxal detoxification by glyoxalase system: A survival strategy during environmental stresses. Physiol Mol Biol Plants 11:1–11Google Scholar