Transgenic Research

, Volume 16, Issue 6, pp 771–781 | Cite as

pORE: a modular binary vector series suited for both monocot and dicot plant transformation

  • Catherine Coutu
  • James Brandle
  • Dan Brown
  • Kirk Brown
  • Brian Miki
  • John Simmonds
  • Dwayne D. Hegedus
Original Paper


We present a series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotyledonous plants. The vector size has been minimized by eliminating all non-essential elements from the vector backbone and T-DNA regions while maintaining the ability to replicate independently. The smallest of the vector series is 6.3 kb and possesses an extensive multiple cloning site with 21 unique restriction endonuclease sites that are compatible with common cloning, protein expression, yeast two-hybrid and other binary vectors. The T-DNA region was engineered using a synthetic designer oligonucleotide resulting in an entirely modular system whereby any vector element can be independently exchanged. The high copy number ColE1 origin of replication has been included to enhance plasmid yield in Escherichia coli. FRT recombination sites flank the selectable marker cassette regions and allow for in planta excision by FLP recombinase. The pORE series consists of three basic types; an ‘open’ set for general plant transformation, a ‘reporter’ set for promoter analysis and an ‘expression’ set for constitutive expression of transgenes. The sets comprise various combinations of promoters (PHPL, PENTCUP2 and PTAPADH), selectable markers (nptII and pat) and reporter genes (gusA and smgfp).


Agrobacterium tumefaciens Binary vector Plant transformation T-DNA Promoter Selectable marker 



Base pair


Deoxyribonucleic acid


Double stranded DNA




Left T-DNA border


Multiple cloning site


Neomycin phosphotransferase II


Oxalate oxidase


Phosphinothricin acetyltransferase


Tobacco cryptic constitutive promoter


Polymerase chain reaction


Arabidopsis thaliana hydroperoxide lyase promoter


Triticum aestivum lipid transfer protein promoter


Triticum aestivum lipid transfer protein promoter fused to an alcohol dehydrogenase intron


Right T-DNA border


Site directed mutagenesis


soluble modified green fluorescent protein


Transferred DNA


Tumour inducing


Polyadenylation signal from the nopaline synthase gene


Virulence region of the Ti plasmid


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Catherine Coutu
    • 1
  • James Brandle
    • 2
  • Dan Brown
    • 2
  • Kirk Brown
    • 2
  • Brian Miki
    • 3
  • John Simmonds
    • 3
  • Dwayne D. Hegedus
    • 1
  1. 1.Agriculture and Agrifood Canada, Saskatoon Research CentreSaskatoonCanada
  2. 2.Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research CenterLondonCanada
  3. 3.Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research CentreOttawaCanada

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