Transgenic Research

, Volume 16, Issue 1, pp 115–120

Establishment and characterization of CAG/EGFP transgenic rabbit line

  • Ri-ichi Takahashi
  • Takashi Kuramochi
  • Kazuki Aoyagi
  • Shu Hashimoto
  • Ichiro Miyoshi
  • Noriyuki Kasai
  • Yoji Hakamata
  • Eiji Kobayashi
  • Masatsugu Ueda
Original Paper

DOI: 10.1007/s11248-006-9043-1

Cite this article as:
Takahashi, R., Kuramochi, T., Aoyagi, K. et al. Transgenic Res (2007) 16: 115. doi:10.1007/s11248-006-9043-1

Abstract

Cell marking is a very important procedure for identifying donor cells after cell and/or organ transplantation in vivo. Transgenic animals expressing marker proteins such as enhanced green fluorescent protein (EGFP) in their tissues are a powerful tool for research in fields of tissue engineering and regenerative medicine. The purpose of this study was to establish transgenic rabbit lines that ubiquitously express EGFP under the control of the cytomegalovirus immediate early enhancer/beta-actin promoter (CAG) to provide a fluorescent transgenic animal as a bioresource. We microinjected the EGFP expression vector into 945 rabbit eggs and 4 independent transgenic candidate pups were obtained. Two of them died before sexual maturation and one was infertile. One transgenic male candidate founder rabbit was obtained and could be bred by artificial insemination. The rabbit transmitted the transgene in a Mendelian manner. Using fluorescence in situ hybridization analysis, we detected the transgene at 7q11 on chromosome 7 as a large centromeric region in two F1 offspring (one female and one male). Eventually, one transgenic line was established. Ubiquitous EGFP florescence was confirmed in all examined organs. There were no gender-related differences in fluorescence. The established CAG/EGFP transgenic rabbit will be an important bioresource and a useful tool for various studies in tissue engineering and regenerative medicine.

Keywords

CAG EGFP Transgenic rabbit Bioresource 

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Ri-ichi Takahashi
    • 1
  • Takashi Kuramochi
    • 1
  • Kazuki Aoyagi
    • 1
  • Shu Hashimoto
    • 1
    • 5
  • Ichiro Miyoshi
    • 2
  • Noriyuki Kasai
    • 3
  • Yoji Hakamata
    • 4
    • 6
  • Eiji Kobayashi
    • 4
  • Masatsugu Ueda
    • 1
  1. 1.The YS Institute Inc., 1198-4 IwazoUtsunomiyaJapan
  2. 2.Center for Experimental Animal ScienceNagoya City University Graduate School of Medical SciencesNagoyaJapan
  3. 3.Institute for Animal experimentationTohoku University Graduate School of MedicineSendaiJapan
  4. 4.Division of Organ Replacement Research, Center for Molecular MedicineJichi Medical SchoolTochigiJapan
  5. 5.IVF Namba Clinic, 1-17-28 Minamihorie, Nishi-kuOsakaJapan
  6. 6.Department of Basic Science, School of Veterinary Nursing and TechnologyNippon Veterinary and Life Science UniversityTokyoJapan

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