Transgenic Research

, Volume 15, Issue 4, pp 455–463 | Cite as

Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies

  • Octavio Guerrero-Andrade
  • Elizabeth Loza-Rubio
  • Teresa Olivera-Flores
  • Tamás Fehérvári-Bone
  • Miguel Angel Gómez-Lim
Original Paper

Abstract

Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of the fusion (F) gene of the Newcastle disease virus (NDV) in transgenic maize plants. The expression of the transgene, driven by the maize ubiquitin promoter, caused accumulation of the F protein in maize kernels. The presence of the transgene was verified by Southern and western blots. Feeding chickens with kernels containing the F protein induced the production of antibodies, which conferred protection against a viral challenge. This protection was comparable to that conferred by a commercial vaccine. Possible uses of this plant-based F protein as a potential mucosal vaccine are discussed.

Keywords

Transgenic plants Recombinant proteins Maize Newcastle disease virus Chickens 

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Octavio Guerrero-Andrade
    • 1
  • Elizabeth Loza-Rubio
    • 2
  • Teresa Olivera-Flores
    • 3
  • Tamás Fehérvári-Bone
    • 4
  • Miguel Angel Gómez-Lim
    • 1
  1. 1.Departamento de Ingeniería Genética de PlantasCinvestav Campus GuanajuatoIrapuato, GuanajuatoMéxico
  2. 2.Centro Nacional de Investigaciones Disciplinarias en Microbiología AnimalINIFAP. Carretera FederalColonia Palo AltoMéxico DF
  3. 3.Facultad de Química, Conjunto E, Laboratorio 116Paseo de la Investigación Científica, Universidad Nacional Autónoma de MéxicoMéxico DF
  4. 4.Departamento de Producción Animal: Aves. Facultad de Medicina Veterinaria y ZootecniaUniversidad Nacional Autónoma de MéxicoMéxico DF

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