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Transgenic Research

, 15:349 | Cite as

Spider Venom Toxin Protects Plants from Insect Attack

  • Sher Afzal Khan
  • Yusuf Zafar
  • Rob W. Briddon
  • Kauser Abdulla Malik
  • Zahid Mukhtar
Article

Abstract

Many of the toxin proteins, that have been heterogeneously expressed in agricultural crops to provide resistance to insect pests, are too specific or are only mildly effective against the major insect pests. Spider venoms are a complex cocktail of toxins that have evolved specifically to kill insects. Here we show that the ω-ACTX-Hv1a toxin (Hvt), a component of the venom of the Australian funnel web spider (Hadronyche versuta) that is a calcium channel antagonist, retains its biological activity when expressed in a heterologous system. Expressed as a fusion protein in E. coli, the purified toxin fusion immobilized and killed Helicoverpa armigera and Spodoptera littoralis caterpillars when applied topically. Transgenic expression of Hvt in tobacco effectively protected the plants from H. armigera and S. littoralis larvae, with 100% mortality within 48 h. We conclude that the Hvt is an attractive and effective molecule for the transgenic protection of plants from herbivorous insects which should be evaluated further for possible application in agriculture.

Keywords

insect Hadronyche versuta resistance spider toxin transgenic 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Sher Afzal Khan
    • 1
  • Yusuf Zafar
    • 1
  • Rob W. Briddon
    • 1
  • Kauser Abdulla Malik
    • 1
  • Zahid Mukhtar
    • 1
  1. 1.Plant Biotechnology DivisionNational Institute for Biotechnology and Genetic Engineering (NIBGE)FaisalabadPakistan

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