Transgenic Research

, Volume 14, Issue 5, pp 719–727 | Cite as

Development of Transgenic Mice Expressing Calcitonin as a Beta-lactoglobulin Fusion Protein in Mammary Gland

  • Ahmadreza Niavarani
  • Somaye Dehghanizadeh
  • Sirous Zeinali
  • Mohsen Karimi
  • Marc Magliano
  • Minoo Rassoulzadegan
Article
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Accepted:
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Abstract

Expression of foreign proteins in mammalian milk is becoming a widespread strategy for high-level production of recombinant pharmaceuticals, especially those with the most complex post-translational modifications. A milk-specific ovine beta-lactoglobulin (oBLG) promoter was used to drive expression of recombinant calcitonin in mouse milk. A gene construct was generated, consisting of 10.7 kbp of the oBLG gene including its promoter and 3′ flanking region with the calcitonin coding sequences inserted in-frame into the oBLG fifth exon. After microinjection, six founder mice transmitted the transgene to their progeny. RT-PCR confirmed mammary-gland specific expression of recombinant mRNA in most transgenic mice and Western blot analysis confirmed expression of chimeric protein. Calcitonin can thus be expressed under the oBLG promoter and regulatory elements in a mammary-gland specific manner.

Keywords

beta-lactoglobulin calcitonin fusion protein milk recombinant protein transgenic mice 
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Copyright information

© Springer 2005

Authors and Affiliations

  • Ahmadreza Niavarani
    • 1
  • Somaye Dehghanizadeh
    • 1
  • Sirous Zeinali
    • 1
  • Mohsen Karimi
    • 1
  • Marc Magliano
    • 2
  • Minoo Rassoulzadegan
    • 2
  1. 1.Department of BiotechnologyPasteur Institute of IranTehranIran
  2. 2.INSERM U636University of Nice-Sophia AntipolisAntipolisFrance

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