Transgenic Research

, Volume 15, Issue 1, pp 101–106 | Cite as

Variable Recombination Efficiency in Responder Transgenes Activated by Cre Recombinase in the Vasculature

  • Bochiwe Hara-Kaonga
  • Yu Alice Gao
  • Matthew Havrda
  • Anne Harrington
  • Ivy Bergquist
  • Lucy Liaw
Short Communication

Abstract

Cre recombinase has become a ubiquitous tool in transgenic strategies for regulation of transgene expression in a tissue-specific manner. We report analysis of two SM22αCre lines and their ability to mediate genomic recombination in five independent Cre-responsive transgenic lines. One of the SM22αCre lines developed was a tet-on system based on the reverse tetracycline transactivator. Our goal was to use this strategy to inhibit the Notch signaling pathway specifically in smooth muscle cells. Our responder transgenes contained a constitutively expressed marker gene (chloramphenicol acetyltransferase, CAT), flanked by loxP sites in direct orientation, upstream of Notch-related transgenes. We developed two dominant negative Notch transgenic responder lines activated by Cre-mediated DNA recombination. The first is the extracellular domain of human Jagged1, and the second is the extracellular domain of the human Notch2 receptor. Despite high expression of the marker gene in all responder lines, we found that Cre-mediated genomic recombination between these five lines was highly variable, ranging from 46 to 93% of individuals using an SM22αCre activating strain, or 8–58% of individuals using an inducible SM22αrtTACre. In all cases examined, detection of recombination by PCR correlated with expression of the transgene as determined by Western blot analysis. Our studies reflect the variability in recombination success based on the responder strain, presumably due to inaccessibility of the locus of integration of the responder allele.

Keywords

Cre recombinase Jagged1 Notch reverse tetracycline transactivator SM22α vascular 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Bochiwe Hara-Kaonga
    • 1
  • Yu Alice Gao
    • 1
    • 2
  • Matthew Havrda
    • 1
  • Anne Harrington
    • 1
  • Ivy Bergquist
    • 1
  • Lucy Liaw
    • 1
  1. 1.Center for Molecular MedicineMaine Medical Center Research InstituteScarboroughUSA
  2. 2.Corning Life SciencesKennebunkUSA

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