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Transgenic Research

, Volume 14, Issue 5, pp 615–618 | Cite as

Modification of Cytokinin Levels in Potato via Expression of the Petunia hybrida Sho Gene

  • Elena Zubko
  • Ivana Macháčková
  • Jiri Malbeck
  • Peter Meyer
Short communication

Abstract

The Sho gene from Petunia hybrida encodes an enzyme for cytokinin synthesis. Here we report on the effects of Shogene expression on potato development. In contrast to transgenic potato expressing the Agrobacterium ipt gene, moderate Sho expression resulted in sufficient root development that allowed the cultivation of the Sho transformants in soil. The most pronounced effects detectable in these lines were an enhanced shoot production, delayed tuber formation, significant reduction in tuber size, and inhibition of tuber dormancy. Sho expression predominantly associated with a strong increase in 2iP glucosides, accompanied by an increase in zeatin glucosides in lines with very high Sho expression levels. The data demonstrate that it is possible to produce viable plants with enhanced cytokinin levels via constitutive Sho expression, which allows an assessment of cytokinin effects in all organs.

Keywords

cytokinins ipt petunia potato Sho gene 

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References

  1. Galis, I, Macas, J, Vlasak, J, Ondrej, M, Vanonckelen, HA 1995The Effect of an elevated Cytokinin level using the Ipt Gene and N-6-Benzyladenine on single node and intact potato plant tuberization in-vitroJ Plant Growth Regul14143150Google Scholar
  2. Haberer, G, Kieber, JJ 2002Cytokinins. New insights into a classic phytohormonePlant Physiol128354362CrossRefPubMedGoogle Scholar
  3. Hedden, P, Phillips, AL 2000Manipulation of hormone biosynthetic genes in transgenic plantsCurr Opin Biotech11130137CrossRefPubMedGoogle Scholar
  4. Lafta A, Wu J and Lorenzen J (2003) Transformation of potato plants with the isopentenyltransferase gene leads to enhanced stolon development and precocious tuber sprouting. Biotechnology in Horticultural Crop Improvement: Achievements, Opportunities and Limitations (pp. 355–361).Google Scholar
  5. Machackova, I, Sergeeva, L, Ondrej, M, Zaltsman, O, Konstantinova, TN, Eder, J, Ovesna, J, Golyanovskaya, SA, Rakitin, Y, Aksenova, NP 1997Growth pattern, tuber formation and hormonal balance in in vitro potato plants carrying ipt genePlant Growth Regul212736CrossRefGoogle Scholar
  6. Mok, DWS, Mok, MC 2001Cytokinin metabolism and actionAnnu Rev Plant Physiol Plant Mol Biol5289118CrossRefPubMedGoogle Scholar
  7. Romanov, GA, Aksenova, NP, Konstantinova, TN, Golyanovskaya, SA, Kossmann, J, Willmitzer, L 2000Effect of indole-3-acetic acid and kinetin on tuberisation parameters of different cultivars and transgenic lines of potato in vitroPlant Growth Regul32245251CrossRefGoogle Scholar
  8. Suttle, J 2001Dormancy-related changes in cytokinin efficacy and metabolism in potato tubers during postharvest storagePlant Growth Regul35199206CrossRefGoogle Scholar
  9. Zubko, E, Adams, CJ, Macháèková, I, Malbeck, J, Scollan, C, Meyer, P 2002Activation tagging identifies a gene from Petunia hybrida responsible for the production of active cytokinins in plantsPlant J29797808CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Elena Zubko
    • 1
  • Ivana Macháčková
    • 2
  • Jiri Malbeck
    • 2
  • Peter Meyer
    • 1
  1. 1.Centre for Plant SciencesThe University of LeedsUK
  2. 2.Institute of Experimental BotanyPrahaCzech Republic

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