Assessing the Transfer of Genetically Modified DNA from Feed to Animal Tissues
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In Europe, public and scientific concerns about the environmental and food safety of GM (Genetically Modified) crops overshadow the potential benefits offered by crop biotechnology to improve food quality. One of the concerns regarding the use of GM food in human and animal nutrition is the effect that newly introduced sequences may have on the organism. In this paper, we assess the potential transfer of diet-derived DNA to animal tissues after consumption of GM plants. Blood, spleen, liver, kidney and muscle tissues from piglets fed for 35 days with diets containing either GM (MON810) or a conventional maize were investigated for the presence of plant DNA. Only fragments of specific maize genes (Zein, Sh-2) could be detected with different frequencies in all the examined tissues except muscle. A small fragment of the Cry1A(b) transgene was detected in blood, liver, spleen and kidney of the animals raised with the transgenic feed. The intact Cry1A(b) gene or its minimal functional unit were never detected. Statistical analysis of the results showed no difference in recovery of positives for the presence of plant DNA between animals raised with the transgenic feed and animals raised with the conventional feed, indicating that DNA transfer may occur independently from the source and the type of the gene. From the data obtained, we consider it unlikely that the occurrence of genetic transfer associated with GM plants is higher than that from conventional plants.
Key words:cry1A(b) DNA transfer Shrunken-2 Zein
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- Armstrong, CL, Parker, GB, Pershing, JC, Brown, SM, Sanders, PR, Duncan, DR, et al. 1995Field evaluation of European corn borer control in progeny of 173 transgenic corn events expressing an insecticidal protein from Bacillus thuringiensisCrop Science35550557Google Scholar
- Beever, DE, Kemp, CF 2000Safety issues associated with the DNA in animal feed derived from genetically modified crops. A review of scientific and regulatory proceduresNutr Abs Rev Series B70175182Google Scholar
- Brown SM, Dean DA, Fromm ME, and Sanders PR (1997) Synthetic DNA sequences having enhanced expression in monocotyledonous plants and method for preparation thereof. United States Patent Application No. 5689052. Assignee: Monsanto Company.Google Scholar
- Reuter, T, Aulrich, K 2002Investigations on genetically modified maize (Bt-maize) in pig nutrition: fate of feed-ingested foreign DNA in pig bodiesEur Food Res Technol216185192Google Scholar
- Sambrook, J, Fritsch, EF, Maniatis, T 1989Molecular Cloning: A Laboratory ManualCold Spring Harbor Laboratory PressNew York931957Google Scholar
- Shaw, JL, Hannah, LC 1992Genomic nucleotide sequence of a wild-type shrunken-2 allele of Zea maysPlant Physiol9812141216Google Scholar
- SPSS Inc., (2003) http://www.spss.com, 233 S.Wacker Drive, 11th floor, Chicago, IL 60606.Google Scholar