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Transgenic Research

, Volume 14, Issue 2, pp 145–158 | Cite as

A 470 bp WAP-promoter fragment confers lactation independent, progesterone regulated mammary-specific gene expression in transgenic mice

  • Karoline Lipnik
  • Helga Petznek
  • Ingrid Renner-Müller
  • Monika Egerbacher
  • Angelika Url
  • Brian Salmons
  • Walter H. Günzburg
  • Christine Hohenadl
Article

Abstract

The ability of a 470 bp sub-fragment of the murine whey acidic protein (WAP) promoter in the context of a retroviral expression plasmid to direct gene expression to mammary epithelial cells was analysed in a number of independent transgenic mouse lines. In contrast to previous findings with the genuine 2.5 kb promoter fragment, our studies revealed a highly mammary gland-specific expression detectable only in non-lactating animals. This suggested a mainly progesterone-regulated activity of the short fragment. Therefore, transgene expression was examined in the progesterone-determined estrous cycle and during pregnancy. In accordance with in vitrodata from stably transfected cell lines, in both situations expression was upregulated at stages associated with high progesterone levels. Taken together these data provide deeper insight into WAP-promoter regulation and stress the usefulness of the shortened fragment for a lactation independent mammary-targeted expression.

Keywords

hormone regulation mammary-specific expression murine WAP-promoter progesterone transgenic mice 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Karoline Lipnik
    • 1
  • Helga Petznek
    • 1
  • Ingrid Renner-Müller
    • 3
  • Monika Egerbacher
    • 2
  • Angelika Url
    • 2
  • Brian Salmons
    • 4
  • Walter H. Günzburg
    • 1
  • Christine Hohenadl
    • 1
    • 4
  1. 1.Research Institute for Virology and BiomedicineAustria
  2. 2.University of Veterinary MedicineViennaAustria
  3. 3.Institute of Molecular Animal Breeding and BiotechnologyLudwig-Maximilian-UniversityMunichGermany
  4. 4.AustrianovaViennaAustria

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