Transgenic Research

, Volume 13, Issue 6, pp 541–549

Overexpression of human erythropoietin (EPO) affects plant morphologies: retarded vegetative growth in tobacco and male sterility in tobacco and Arabidopsis

  • Ban Yoon Cheon
  • Hae Jin Kim
  • Kyung Hee Oh
  • Sung Chul Bahn
  • Ji Hoon Ahn
  • Jang Won Choi
  • Sung Han Ok
  • Jung Myung Bae
  • Jeong Sheop Shin
Article

Abstract

Erythropoietin (EPO) is a glycoprotein used for curing human anemia by regulating the differentiation of erythroid progenitors and the production of red blood cells. To examine the expression of recombinant EPO in plants, pPEV-EP21, in which human epo cDNA under the control of the CaMV 35S promoter, was introduced into tobacco and Arabidopsisvia Agrobacterium tumefaciens-mediated transformation. The RNA expression level of epo in the transgenic lines was initially estimated by Northern blot analysis. Two transgenic lines, which exhibited a high expression level of epo mRNA determined by Northern analysis, were chosen for Western blot analysis to examine the production of EPO proteins. Those two lines, EP21-12 and EP21-14, revealed detectable bands on the immunoblot. Interestingly, constitutive expression of the human epo gene affected the morphologies in transgenic plants such that vegetative growth of transgenic tobacco was retarded, and male sterility was induced in transgenic tobacco and Arabidopsis

Keywords

Arabidopsis thaliana dwarfism erythropoietin (EPO) male sterility Nicotiana tabacum overexpression phenotypic change 

Abbreviations

cDNA

complementary DNA

CaMV

cauliflower mosaic virus

EPO

erythropoietin

PCR

polymerase chain reaction

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Ban Yoon Cheon
    • 1
  • Hae Jin Kim
    • 1
  • Kyung Hee Oh
    • 1
  • Sung Chul Bahn
    • 1
  • Ji Hoon Ahn
    • 1
  • Jang Won Choi
    • 2
  • Sung Han Ok
    • 1
  • Jung Myung Bae
    • 1
  • Jeong Sheop Shin
    • 1
  1. 1.School of Life Sciences and BiotechnologyKorea UniversitySeoulKorea
  2. 2.Division of Life Science, College of Natural ResourcesTaegu UniversityKyungsanKorea

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