Advertisement

Topics in Catalysis

, Volume 61, Issue 15–17, pp 1665–1671 | Cite as

Degradation of Pyrene Contaminated Soil with Spiked 14C Pyrene by Hemoglobin Catalysis

  • Guyoung Kang
  • Sungjong Lee
  • Haein Keum
  • Namhyun Chung
Original Paper

Abstract

Hemoglobin (Hb) is a member of the hemeprotein family that undergoes non-specific catalytic chain reactions in the presence of hydrogen peroxide (H2O2). The catalytic ability of Hb to degrade the carcinogenic polycyclic aromatic hydrocarbon pyrene was demonstrated using soil contaminated with 14C pyrene. Three bench-scale laboratory tests were performed using 14C pyrene in the presence of a buffer, H2O2, and a combination of Hb and H2O2. The initial pyrene concentration of the contaminated soil was set to 11 mg/kg, with 5,500,000 dpm of 14C pyrene. After a catalytic reaction for 24 h, the results showed that 17% of pyrene was degraded by H2O2, 38% of pyrene was degraded by Hb in combination with H2O2, and 0.13 and 1.2% of 14C pyrene were mineralized by H2O2 and Hb in combination with H2O2, respectively. An analysis of the products from the reaction involving Hb in combination with H2O2 revealed that 15.9% of the 14C intermediates in the acetonitrile fraction were polar products. After the catalytic reaction, 21 intermediate compounds were found via fraction analysis. The results suggested that Hb catalysis could be used to treat pyrene-contaminated soil as a novel catalytic technology for the remediation of hazardous materials in soil.

Keywords

Hemoglobin Hydrogen peroxide Mass balance 14C pyrene Remediation 

Notes

Acknowledgements

This study was supported by the Hankuk University of Foreign Studies (2017).

References

  1. 1.
    Haritash A, Kaushik C (2009) Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. J Hazard Mater 169:1–15CrossRefGoogle Scholar
  2. 2.
    Peng R, Xiong A, Gao Y, Zhao W, Tian Y, Yao Q (2008) Microbial biodegradation of polyaromatic hydrocarbons. FEMS Microbiol Rev 32:927–955CrossRefGoogle Scholar
  3. 3.
    Yu K, Wong A, Yau K, Wong Y, Tam N (2005) Natural attenuation, biostimulation and bioaugmentation on biodegradation of polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments. Mar Pollut Bull 51:1071–1077CrossRefGoogle Scholar
  4. 4.
    Bamforth S, Singleton I (2005) Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions. J Chem Technol Biotechnol 80:723–736CrossRefGoogle Scholar
  5. 5.
    McNally D, Mihelcic J, Lueking D (1998) Biodegradation of three- and four-ring polycyclic aromatic hydrocarbons under aerobic and denitrifying conditions. Environ Sci Technol 32:2633–2639CrossRefGoogle Scholar
  6. 6.
    Mohamed I, Khalil N, El-Ghany M (2012) Biodegradation of some polycyclic aromatic hydrocarbons by Aspergillus terreus. Afr J Microbiol Res 6:3783–3790CrossRefGoogle Scholar
  7. 7.
    Atagana H, Haynes R, Wallis F (2006) Fungal bioremediation of creosote-contaminated soil: a laboratory scale bioremediation study using indigenous soil fungi. Water Air Soil Pollut 172:201–219CrossRefGoogle Scholar
  8. 8.
    Straube W, Nestler W, Hansen C, Ringleberg L, Pritchard D, Jonesmeehan P (2003) Remediation of polyaromatic hydrocarbons (PAHs) through landfarming with biostimulation and bioaugmentation. Acta Biotechnol 23:179–196CrossRefGoogle Scholar
  9. 9.
    Luo S, Chen B, Lin L, Wang X, Tam N, Luan T (2014) Pyrene degradation accelerated by constructed consortium of bacterium and microalga: effects of degradation products on the microalgal growth. Environ Sci Technol 48:13917–13924CrossRefGoogle Scholar
  10. 10.
    Bumpus J (1989) Biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium. Appl Environ Microbiol 55:154–158PubMedPubMedCentralGoogle Scholar
  11. 11.
    Hammel K, Kalyanaraman B, Kirk T (1986) Oxidation of polycyclic aromatic hydrocarbons and dibenzop[p]dioxins by Phanerochaete chrysosporium ligninase. J Biol Chem 261:16948–16952PubMedGoogle Scholar
  12. 12.
    Sack U, Heinze T, Deck J, Cerniglia C, Martens R, Zadrazil F, Fritsche W (1997) Comparison of phenanthrene and pyrene degradation by different wood-decaying fungi. Appl Environ Microbiol 63:3919–3925PubMedPubMedCentralGoogle Scholar
  13. 13.
    Kanaly R, Harayama S (2010) Advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria. Microb Biotechnol 3:136–164CrossRefGoogle Scholar
  14. 14.
    Cerniglia C (1992) Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3:351–368CrossRefGoogle Scholar
  15. 15.
    Chung N, Park K, Stevens D, Kang G (2014) Verification of heme catalytic cycle with 5-aminosalicylic acid and its application to soil remediation of polycyclic aromatic hydrocarbons. Environ Eng Res 19:139–143CrossRefGoogle Scholar
  16. 16.
    Kang G, Park K, Cho J, Stevens D, Chung N (2015) Remediation of polycyclic aromatic hydrocarbons in soil using hemoglobin-catalytic mechanism. J Environ Eng 141:04015025.  https://doi.org/10.1061/(ASCE)EE.1943-7870.0000955 CrossRefGoogle Scholar
  17. 17.
    Keum H, Kang G, Jho E (2017) Optimization of hydrogen peroxide-to-hemoglobin ratio for biocatalytic mineralization of polycyclic aromatic hydrocarbons (PAHs)-contaminated soils. Chemosphere 187:206–211CrossRefGoogle Scholar
  18. 18.
    Chen S, Stevens D, Kang G (1999) Pentachlorophenol and crystal violet degradation in water and soils using heme and hydrogen peroxide. Water Res 33:3657–3665CrossRefGoogle Scholar
  19. 19.
    Chen S, Stevens D, Kang G, Hsieh M (2006) Treating soil PCP at optimal conditions using heme and peroxide. J Environ Eng 132:704–708CrossRefGoogle Scholar
  20. 20.
    Keum H, Kang G, Chung N (2017) Oxidation of pyrene using a hemoglobin-catalyzed biocatalytic reaction. Appl Biol Chem 60:401–405CrossRefGoogle Scholar
  21. 21.
    Ghosal D, Ghosh S, Dutta T, Ahn Y (2016) Current state of knowledge in microbial degradation of polycyclic aromatic hydrocarbons (PAHs): a review. Front Microbiol 7:1369.  https://doi.org/10.3389/fmicb.2016.01369 CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Peng R, Xiong A, Xue Y. Fu X, Gao F, Zhao W, Tian Y, Yao Q (2008) Microbial biodegradation of polyaromatic hydrocarbons. FEMS Microbiol Rev 32:927–955CrossRefGoogle Scholar
  23. 23.
    Joo Y, Lee D, Lee N, Chung N (2016) Model development for prediction of the allergic response to the wheat proteins ω-5 gliadin and HMW-glutenin. Appl Biol Chem 59:827–831CrossRefGoogle Scholar
  24. 24.
    Araghi M, Olya M, Marandi R, Siadat S (2016) Investigation of enhanced biological dye removal of colored wastewater in a lab-scale biological activated carbon process. Appl Biol Chem 59:463–470CrossRefGoogle Scholar
  25. 25.
    Jung Y, Lim W, Park J, Kim Y (2009) Effect of pH on Fenton and Fenton-like oxidation. Environ Technol 30:183–190CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Environmental ScienceHankuk University of Foreign StudiesYoungin-siRepublic of Korea
  2. 2.Department of Biosystems and Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea

Personalised recommendations