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Synthesis of a Re-usable Cellobiase Enzyme Catalyst through In situ Encapsulation in Nonsurfactant Templated Sol–Gel Mesoporous Silica

Abstract

We present a re-usable enzyme catalyst system via direct encapsulation of cellobiase in nonsurfactant templated sol–gel mesoporous silica host material with d-fructose as the template. The pore diameter and porosity of the silica host material, controlled by the fructose content, controlled the diffusion of substrate to the enzyme. This in situ immobilized cellobiase showed little or no leakage while could be repeatedly used as biocatalyst with little or no loss of activity after at least 9 cycles.

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Acknowledgments

We are indebted to Prof. Yury Gogotsi of the Department of Materials Science and Engineering, Drexel University, for letting us use his BET instrument. We are grateful to Prof. Jun Xi of the Chemistry Department, Drexel University, for discussions and valuable suggestions. We thank the Chinese Ministry of Science and Technology for the support via the National 973 Program (Grant No. 2011CB935700).

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Correspondence to Yen Wei.

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Wei, Y., Das, S., Berke-Schlessel, D. et al. Synthesis of a Re-usable Cellobiase Enzyme Catalyst through In situ Encapsulation in Nonsurfactant Templated Sol–Gel Mesoporous Silica. Top Catal 55, 1247–1253 (2012). https://doi.org/10.1007/s11244-012-9892-1

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Keywords

  • Enzyme immobilization
  • Cellobiase enzyme
  • Mesoporous silica
  • Nonsurfactant template
  • Sol–gel reactions