Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Synthesis, DNA binding, antioxidant and cytotoxic activities of ruthenium(II) complexes of a Schiff base ligand


Three ruthenium(II) complexes, [Ru(CO)Cl(PPh3)L], [Ru(CO)Cl(AsPh3)L] and [Ru(CO)Cl(Py)L], were synthesized from the reactions of 2-(benzothiazol-2-yliminomethyl)-phenol (HL) with [RuHCl(CO)B(EPh3)2], where B = PPh3, AsPh3 or pyridine, and E = P or As. All the complexes have been characterized by physicochemical and spectroscopic methods. The structure of the free ligand HL was determined by single crystal X-ray diffraction. The binding of the free ligand and its complexes with CT-DNA was studied using electronic absorption spectroscopy. In addition, the free ligand and its complexes were subjected to antioxidant activity tests, which showed that they all possess significant scavenging effects against DPPH and OH radicals. The in vitro cytotoxicities of the compounds were assessed using tumor (HeLa and MCF-7) cell lines.

This is a preview of subscription content, log in to check access.

Fig. 1
Scheme 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5


  1. 1.

    Tolomeo M, Simoni D (2002) Curr Med Chem Anti Cancer Agents 2:387

  2. 2.

    Coury JE, Anderson JR, Isom LM, Williams LD, Bottomley LA (1997) J Am Chem Soc 119:3792

  3. 3.

    Trynda-Lemiesz L (2004) Acta Biochim 52(1):199

  4. 4.

    Bergamo A, Stocco G, Casassa C, Cocchettio M, Alessio E, Serli B, Zorzet S, Sava G (2004) Int J Oncol 24:373

  5. 5.

    Mann J, Baron A, Opoku-Boahen Y, Johansson E, Parkinson G, Kelland LR, Neidle S (2001) J Med Chem 44:138

  6. 6.

    Chua MS, Kachiyama E, Bradshaw TD, Stinson SF, Brantly E, Sausille EA, Stevens MFB (2000) Cancer Res 60:5196

  7. 7.

    Vogal AI (1989) Text book of practical organic chemistry, 5th edn. Longman, London

  8. 8.

    Ahmed N, Levison JJ, Robinson SD, Uttley MF (1974) Inorg Synth 15:48

  9. 9.

    Sanchez-Delgade RA, Lee WY, Choi SR, Cho YMJ, Jun Y (1991) Trans Met Chem 16:41

  10. 10.

    Gopinathan S, Unny IR, Deshpande SS, Gopinathan C (1986) Ind J Chem A 25:1015

  11. 11.

    Sheldrick GM, SHELXS-97, SHELX-97 (1997) Fortran programs for crystal structure solution and refinement. University of Gottingen, Germany

  12. 12.

    Wolf A, Shimer GH, Meehan T (1987) Biochem 26:6392

  13. 13.

    Elizabeth K, Rao MNA (1990) Int J Pharmaceut 58:237

  14. 14.

    Yu W, Zhao Y, Shu B (2004) Food Chem 86:525

  15. 15.

    Blagosklonny M, EL-Diery WS (1996) Int J Cancer 67:386

  16. 16.

    Ramesh R, Sivagamasundari M (2003) Synth React Inorg Met-Org Chem 33:899

  17. 17.

    Nakamoto K (1971) Infrared and Raman spectra of inorganic and co-ordination compounds. Wiley Interscience, New York

  18. 18.

    Das AK, Peng SM, Bhattacharya SJ (1976) Chem Soc Jpn 49:287

  19. 19.

    Sharma RK, Singh RV, Tandon JPJ (1980) Inorg Nucl Chem 42:1382

  20. 20.

    Natarajan K, Poddar RK, Agarwala CJ (1977) Inorg Nucl Chem 39:431

  21. 21.

    Lever ABP (1984) Inorganic electronic spectroscopy, 2nd edn. Elsevier, New York

  22. 22.

    Maji M, Ghosh S, Chattopaghyay SK, Mak TCW (1997) Inorg Chem 36:2938

  23. 23.

    Singh RV, Joshi SC, Gajraj A, Nagpal P (2002) Appl Organomet Chem 16:713

  24. 24.

    Navarro M, Cisneros-Fajardo EJ, Sierralta A, Fernández-Mestre M, Silva P, Arrieche D, Marchán E (2003) J Biol Inorg Chem 8:401

  25. 25.

    Perz S, Lopez C, Caubet A, Solans X, Bardia MF, Gich M, Molins E (2007) J Organomet Chem 692:2402

  26. 26.

    Shankera K, Rohinia R, Ravindera V, Reddy PM, Ho Y-P (2009) Spectrochim. Acta A 73:205

  27. 27.

    Desbosis MH, Astruc D (1989) Organometallics 8:1841

  28. 28.

    Karvembu R, Natarajan K (2002) Polyhedron 21:1721

  29. 29.

    Long EC, Barton JK (1990) Acc Chem Res 23:271

  30. 30.

    Chitrapriya N, Mahalingam V, Zeller M, Natarajan K (2010) Inorg Chim Acta 363:3685

  31. 31.

    Liu XW, Li J, Deng H, Zheng KC, Mao ZW, Ji LN (2005) Inorg Chim Acta 358:3311

  32. 32.

    Liang ZH, Li ZZ, Huang HL, Liu YJ (2011) J Coord Chem 64:3342

Download references


We sincerely thank University Grants Commission (UGC), New Delhi, for financial support [MRP Scheme. No. 38-222/2009 (SR)].

Author information

Correspondence to Chinnasamy Jayabalakrishnan.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sathiyaraj, S., Sampath, K., Butcher, R.J. et al. Synthesis, DNA binding, antioxidant and cytotoxic activities of ruthenium(II) complexes of a Schiff base ligand. Transition Met Chem 38, 291–298 (2013).

Download citation


  • DPPH
  • Ruthenium
  • Schiff Base
  • PPh3
  • Radical Scavenge Activity