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Transition Metal Chemistry

, Volume 36, Issue 8, pp 867–874 | Cite as

DNA-binding and nuclease activity of 1,10-phenanthroline-based thiocyanato, acetato, azido and benzoato bridged dinuclear copper(II) complexes

  • Pagonda Haribabu
  • Yogesh Prakash Patil
  • Katreddi Hussain ReddyEmail author
  • Munirathinam Nethaji
Article

Abstract

The mononuclear Cu(II) complex [Cu(phen)(H2O)(NO3)2] (1), obtained by the reaction of 1,10-phenanthroline with Cu(NO3)2·3H2O in methanol solution, reacts with anionic ligands SCN, AcO, N3 and PhCO2 in MeOH solution to form the stable binuclear complexes [Cu2(H2O)2(phen)2(μ-X)2]2 (NO3)2, where X = SCN (2), AcO (3), N3 (4) or PhCO2 (5). The molecular structure of complex 3 was determined by single-crystal X-ray diffraction studies. These complexes were characterized by electronic, IR, ESR, magnetic moments and conductivity measurements. The electrochemical behaviour of the complexes was investigated by cyclic voltammetry. The interactions of these complexes with calf thymus DNA have been investigated using absorption spectrophotometry. Their DNA cleavage activity was studied on double-stranded pBR322 plasmid DNA using gel electrophoresis experiments in the absence and presence of H2O2 as oxidant.

Keywords

Phen Liquid Nitrogen Temperature Nuclease Activity TCNE Dinuclear Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are thankful to the DST, New Delhi, (Sanction No. SR/S1/IC-37/2007) for financial support and SAIF (RSIC), Indian Institute of Technology, Bombay, for providing electron spin resonance spectra. Y. P. Patil is thankful to CSIR, New Delhi, for the award of fellowship.

Supplementary material

11243_2011_9543_MOESM1_ESM.docx (13 kb)
Crystallographic data for structural analysis have been deposited with the Cambridge Crystallographic Data Center, CCDC 775674 for complex 3. Copies of this information may be obtained free of charge from the Director, CCDC, 12 Union Road, Cambridge, CB2 IEZ, UK (fax: +44-1223-336-033; e-mail: deposit@ccdc.cam.ac.uk or http://www.ccdc.cam.ac.uk). (DOCX 12 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Pagonda Haribabu
    • 1
  • Yogesh Prakash Patil
    • 2
  • Katreddi Hussain Reddy
    • 1
    Email author
  • Munirathinam Nethaji
    • 2
  1. 1.Department of ChemistrySri Krishnadevaraya UniversityAnantapurIndia
  2. 2.Department of Inorganic and Physical ChemistryIndian Institute of ScienceBangaloreIndia

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