Transition Metal Chemistry

, Volume 36, Issue 8, pp 867–874 | Cite as

DNA-binding and nuclease activity of 1,10-phenanthroline-based thiocyanato, acetato, azido and benzoato bridged dinuclear copper(II) complexes

  • Pagonda Haribabu
  • Yogesh Prakash Patil
  • Katreddi Hussain ReddyEmail author
  • Munirathinam Nethaji


The mononuclear Cu(II) complex [Cu(phen)(H2O)(NO3)2] (1), obtained by the reaction of 1,10-phenanthroline with Cu(NO3)2·3H2O in methanol solution, reacts with anionic ligands SCN, AcO, N3 and PhCO2 in MeOH solution to form the stable binuclear complexes [Cu2(H2O)2(phen)2(μ-X)2]2 (NO3)2, where X = SCN (2), AcO (3), N3 (4) or PhCO2 (5). The molecular structure of complex 3 was determined by single-crystal X-ray diffraction studies. These complexes were characterized by electronic, IR, ESR, magnetic moments and conductivity measurements. The electrochemical behaviour of the complexes was investigated by cyclic voltammetry. The interactions of these complexes with calf thymus DNA have been investigated using absorption spectrophotometry. Their DNA cleavage activity was studied on double-stranded pBR322 plasmid DNA using gel electrophoresis experiments in the absence and presence of H2O2 as oxidant.


Phen Liquid Nitrogen Temperature Nuclease Activity TCNE Dinuclear Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are thankful to the DST, New Delhi, (Sanction No. SR/S1/IC-37/2007) for financial support and SAIF (RSIC), Indian Institute of Technology, Bombay, for providing electron spin resonance spectra. Y. P. Patil is thankful to CSIR, New Delhi, for the award of fellowship.

Supplementary material

11243_2011_9543_MOESM1_ESM.docx (13 kb)
Crystallographic data for structural analysis have been deposited with the Cambridge Crystallographic Data Center, CCDC 775674 for complex 3. Copies of this information may be obtained free of charge from the Director, CCDC, 12 Union Road, Cambridge, CB2 IEZ, UK (fax: +44-1223-336-033; e-mail: or (DOCX 12 kb)


  1. 1.
    Elerman Y, Kara H, Elmali A (2003) Z Naturforch 58a:363Google Scholar
  2. 2.
    Bertini I, Gray HB, Lippard SJ, Valentine JS (1995) Bioinorganic Chemistry. University Science Books, Mill ValleyGoogle Scholar
  3. 3.
    Mahapatra S, Halfen JA, Wilkinson EC, Que L Jr, Tolman WB (1994) J Am Chem Soc 116:9785CrossRefGoogle Scholar
  4. 4.
    Mahapatra S, Halfen JA, Wilkinson EC, Que L Jr, Tolman WB (1995) J Am Chem Soc 117:8865CrossRefGoogle Scholar
  5. 5.
    Mahapatra S, Halfen JA, Wilkinson EC, Pan G, Wang X, Young VG Jr, Cramer CJ, Que L Jr, Tolman WB (1996) J Am Chem Soc 118:11555CrossRefGoogle Scholar
  6. 6.
    Hussain Reddy K, Sambasiva Reddy P (2000) Transition Met Chem 25:505CrossRefGoogle Scholar
  7. 7.
    Dervan PB (1986) Science 464:6232Google Scholar
  8. 8.
    Lu P, Zhu ML, Yang P (2003) J Inorg Biochem 95:31CrossRefGoogle Scholar
  9. 9.
    Wu JZ, Li H, Zhang JG, Xu J (2002) Inorg Chem Commun 5:71CrossRefGoogle Scholar
  10. 10.
    Hemmert C, Pitie M, Gornitzka H, Soulet S, Meunier B (2001) J Biol Inorg Chem 6:14CrossRefGoogle Scholar
  11. 11.
    Navarro M, Cisneros-Fajardo EJ, Sierralta A, Fernandez-Mastre M, Silva P, Arrieche D, Marchan E (2003) J Biol Inorg Chem 8:401Google Scholar
  12. 12.
    Spassky A, Sigman DS (1985) Biochemistry 24:8050CrossRefGoogle Scholar
  13. 13.
    Sigman DS, Mujumder A, Perrin DM (1993) Chem Rev 93:2295CrossRefGoogle Scholar
  14. 14.
    Bencini A, lippolis V (2010) Coord Chem Rev 254:2096CrossRefGoogle Scholar
  15. 15.
    Hussain Reddy K, Surendra Babu M, Surendra Babu P, Dayananda S (2004) Indian J Chem 43A:1233Google Scholar
  16. 16.
    Murali Krishna P, Hussain Reddy K, Pandey JP, Dayananda S (2008) Transition Met Chem 33:661CrossRefGoogle Scholar
  17. 17.
    Haribabu P, Hussain Reddy K (2011) Indian J Chem 50A:996Google Scholar
  18. 18.
    Tu C, Wu X, Liu Q, Wang X, Xu Q, Guo Z (2004) Inorg Chim Acta 357:95CrossRefGoogle Scholar
  19. 19.
    Wu B-Y, Gao L-H, Duan Z-M, Wang K-Z (2005) J Inorg Biochem 99:1685CrossRefGoogle Scholar
  20. 20.
    Siemens, SMART and SAINT (1996) Area detector control and integration software. Siemens Analytical X-ray Instruments. Madison, Wisconsin, USAGoogle Scholar
  21. 21.
    Sheldrick GM (1990) Acta Crystallogr A 46:467CrossRefGoogle Scholar
  22. 22.
    Sheldrick GM, SHELXS-97 (1997) Program for the solution of crystal structures. University of Gottingen, GermanyGoogle Scholar
  23. 23.
    Tokki T, Wantanabe N, Nakashima M, Muto Y, Morooka M, Ohba S, Saito Y (1990) Bull Chem Soc Jpn 63:364CrossRefGoogle Scholar
  24. 24.
    Nakamoto K (1997) Infrared and raman spectra of inorganic and coordination compounds, 5th edn. Wiley, New YorkGoogle Scholar
  25. 25.
    Goher MAS, Yang Q-C, Mak TCW (2000) Polyhedron 19:615CrossRefGoogle Scholar
  26. 26.
    Yatsimirskii KB (1997) Pure Appl Chem 49:115CrossRefGoogle Scholar
  27. 27.
    Tirumavalavan N, Mariya Rayappan S, Akilan P, Kandaswamy M (2004) Indian J Chem Tech 1129:34Google Scholar
  28. 28.
    Philip V, Suni V, Kurup MRP, Nethaji M (2005) Polyhedron 24:1133CrossRefGoogle Scholar
  29. 29.
    Khumhar AA, Rendye SB, West DX, Libert AE (2005) Transition Met Chem 16:276Google Scholar
  30. 30.
    Usha S, Palaniandavar M (1994) J Chem Soc Dalton Trans (15):2277Google Scholar
  31. 31.
    Barton JK, Danishefsky AT, Goldberg JM (1984) J Am Chem Soc 106:2127Google Scholar
  32. 32.
    Tysose SA, Morgan RJ, Barker AD, Strekars TC (1993) J Phys Chem 97:1707CrossRefGoogle Scholar
  33. 33.
    Kelly TM, Tossi AB, Mckonnell DJ, Strekas TC (1985) Nucleic Acid Res 13:6017CrossRefGoogle Scholar
  34. 34.
    Wang Y, Okabe N (2005) Inorg Chim Acta 358:3407CrossRefGoogle Scholar
  35. 35.
    Lu L-P, Zhu M-L, Yang P (2003) J Inorg Biochem 95:31CrossRefGoogle Scholar
  36. 36.
    Pogozelski WK, Tullius TD (1998) Chem Rev 98:1089CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Pagonda Haribabu
    • 1
  • Yogesh Prakash Patil
    • 2
  • Katreddi Hussain Reddy
    • 1
    Email author
  • Munirathinam Nethaji
    • 2
  1. 1.Department of ChemistrySri Krishnadevaraya UniversityAnantapurIndia
  2. 2.Department of Inorganic and Physical ChemistryIndian Institute of ScienceBangaloreIndia

Personalised recommendations