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Biophysical, spectroscopic and biochemical investigation of DNA–Cu(II)-GSH interactions

Abstract

The interaction of DNA with Copper(II)-Glutathione (CuGSH) has been investigated by various biophysical methods. The interaction ratio of DNA and Copper(II)-Glutathione in solution phase has been determined spectrophotometrically and found to be 0.25. EPR spectroscopy and UV–Vis findings suggest that Cu(II)-Glutathione neither bound to the DNA bases covalently nor intercalated, this has further been substantiated by the determination of intrinsic binding constant (2.1 × 102). Viscometric measurements also support this type of binding to DNA by Cu(II)-Glutathione. EPR studies and visible d–d spectra of CuGSH after interaction with DNA, suggested that Copper remained in the Copper(II) state. DNA conformations after interaction with Cu(II)-Glutathione has been determined spectroscopically. Circular dichroism studies revealed that the B conformation of DNA is changed to A after interaction with Cu(II)-Glutathione. This has further been substantiated by thin film IR (Infrared) studies.

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Acknowledgments

Authors are thankful to the University Grants Commission, New Delhi for financial assistance to K.K.M in the form of a MRP NO F-12-10/98 (SR-I), where GP had been a project fellow. We are also thankful to Jadavpur University for assistance.

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Correspondence to Kalyan K. Mukherjea.

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Mukherjea, K.K., Panda, G. & Selim, M. Biophysical, spectroscopic and biochemical investigation of DNA–Cu(II)-GSH interactions. Transition Met Chem 33, 203–210 (2008). https://doi.org/10.1007/s11243-007-9034-y

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Keywords

  • Circular Dichroism
  • Circular Dichroism Spectrum
  • Circular Dichroism Study
  • Circular Dichroism Band
  • Intrinsic Binding Constant