Each species cultured in vitro has specific nutritional requirements on the culture medium. The use of silicon in culture can provide benefits to plants, such as increase of tolerance to water stress, increase of photosynthetic capacity and reduction in transpiration. These effects may be quite relevant for in vitro growth and development of plant and during acclimatization. However, the effectiveness of this nutrient may vary depending on the used sources. The aim of this study was to verify the ideal concentration of the MS culture medium and to evaluate the to evaluate the different sources as well as the function of silicon for in vitro development and acclimatization of gerbera plantlets. Shoots (4–6 cm) of gerbera (Gerbera jamesonii cv. Jaguar Cream) cultured in vitro were inoculated in MS medium with different concentrations of salts (MS ¼, MS ½, MS ¾, MS) supplemented with different silicon sources (potassium silicate, sodium silicate, calcium silicate and silicic acid) at concentrations of 0 (control), 0.25, 0.5, 0.75, and 1 mg L−1. The use of MS ¼ medium and the supplementation of 0.25 g L−1 calcium silicate provided better shoot development. The acclimatization rate was not affected by the origin of plants treated in vitro by different silicon sources, their concentrations, and the used culture medium. Thus, the use of silicon is beneficial for in vitro development of gerberas, however, the effectiveness depends on the used source.
In vitro shoots of Gerbera jamesonii cv. Jaguar Cream regenerate better, shoots are taller and healthier, and root more effectively when are cultured on MS 1/4 supplemented with 0.25 mg L−1 calcium silicate.
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Al-Khayri JM, Naik PM (2017) Date palm micropropagation: advances and applications. Cienc Agrotec 41(4):347–358. https://doi.org/10.1590/1413-70542017414000217
Alves GAC, Campos FR, Bertoncelli DJ, Furlan FF, Freiria GH, Faria RT (2016) Development of Cattleya loddigesii Lindley in vitro with doses of potassium silicate. Agropecuária Técnica 37(1):81–87. https://doi.org/10.25066/agrotec.v37i1.24193
Aranda-Peres AN, Peres LEP, Higashi EN, Martinelli AP (2009) Adjustment of mineral elements in the culture medium for the micropropagation of three Vriesea Bromeliads from the Brazilian Atlantic forest: the importance of calcium. HortScience 44:106–112
Asmar SA, Castro EM, Pasqual M, Pereira FJ, Soares JDR (2013) Changes in leaf anatomy and photosynthesis of micropropagated banana plantlets under different silicon sources. Sci Hortic 161:328–332. https://doi.org/10.1016/j.scienta.2013.07.021
Asmar SA, Soares JD, Silva RAL, Pasqual M, Pio LAS, Castro EM (2015) Anatomical and structural changes in response to application of silicon (Si) 'in vitro' during the acclimatization of banana cv. 'Grand Naine'. Aust J Crop Sci 9(12):1236–1241
Assis FA, Souza GA, Dias GMG, Assis GA, Rodrigues FA, Pasqual M, Costa BNS, Carvalho FJ (2018) Silicon and agar on in vitro development of cockscomb (Amaranthaceae). Pesqui Agropecu Bras 53(1):30–41. https://doi.org/10.1590/S0100-204X2018000100004
Ayub RA, Santos JN, Zanlorensi LA, Jr, Silva DM, Carvalho TC, Grimaldi F (2019). Sucrose concentration and volume of liquid medium on the in vitro growth and development of blackberry cv. Tupy in temporary immersion systems. Cienc Agrotec 43:e007219. https://doi.org/10.1590/1413-7054201943007219
Bhatia R, Singh KP, Jhang T, Sharma TR (2009) Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers. Sci Hortic 119:208–211. https://doi.org/10.1016/j.scienta.2008.07.024
Braga FT, Nunes CF, Favero AC, Pasqual M, Carvalho JGD, Castro EMD (2009) Anatomical characteristics of the strawberry seedlings micropropagated using different sources of silicon. Pesqui Agropecu Bras 44:128–132. https://doi.org/10.1590/S0100-204X2009000200003
Cardoso JC, Teixeira da Silva JA (2012) Micropropagation of gerbera using chlorine dioxide (ClO2) to sterilize the culture medium. In Vitro Cell Dev Biol Plant 48:362–368. https://doi.org/10.1007/s11627-011-9418-8
Chang HS, Chakrabarty D, Hahn EJ, Paek KY (2003) Micropropagation of calla lily (Zantedeschia albomaculata) via in vitro shoot tip proliferation. Vitro Cell Dev Biol Plant 39:129–134. https://doi.org/10.1079/IVP2002362
Chugh S, Guha S, Rao IU (2009) Micropropagation of orchids: a review on the potential of different explants. Sci Hortic 122:507–520. https://doi.org/10.1016/j.scienta.2009.07.016
Colombo RC, Favetta V, Faria RT, Andrade FA, Melem VM (2016) Response of Cattleya forbesii orchid to increasing silicon concentrations in vitro. Rev Caatinga 29(1):18–24. https://doi.org/10.1590/1983-21252016v29n103rc
Cruz CF, Santos WF, Souza CS, Machado MD, Carvalho IF, Rocha DI, Silva ML (2019) In vitro regeneration and flowering of Portulaca grandiflora Hook. Ornam Hortic 25(4):443–449. https://doi.org/10.1590/2447-536X.v25i4.2077
Desai C, Inghalihalli R, Krishnamurthy R (2015) Micropropagation of Anthurium andraeanum—an important tool in floriculture. J Pharmacogn Phytochem 4(3):112–117
Epstein E (1999) Silicon. Annu Rev Plant Physiol Plant Mol Biol 50:641–664. https://doi.org/10.1146/annurev.arplant.50.1.641
Ferreira DF (2014) Sisvar: a guide for its bootstrap procedures in multiple comparisons. Cienc Agrotec 38(2):109–112. https://doi.org/10.1590/S1413-70542014000200001
Hartley SE (2015) Round and round in cycles? Silicon-based plant defenses and vole population dynamics. Funct Ecol 29:151–153. https://doi.org/10.1111/1365-2435.12365
He C, Wang L, Liu J, Liu X, Li X, Ma J, Lin Y, Xu F (2013) Evidence for ‘silicon’ within the cell walls of suspension-cultured rice cells. New Phytol 200:700–709. https://doi.org/10.1111/nph.12401
Kulpa D (2016) Micropropagation of calla lily (Zantedeschia rehmannii). Folia Hortic 28(2):181–186. https://doi.org/10.1515/fhort-2016-0021
Lim MY, Lee EJ, Jana S, Sivanesan I, Jeong BR (2012) Effect of potassium silicate on growth and leaf epidermal characteristics of begonia and pansy grown in vitro. Hortic Sci Technol 30:579–585. https://doi.org/10.7235/hort.2012.1206
Luz JMQ, Asmar SA, Pasqual M, De Araujo AG, Pio LAS, Resende RF (2012) Modification in leaf anatomy of banana plants cultivar “Maca” subjected to different silicon sources in vitro. Acta Hortic 961:239–243. https://doi.org/10.17660/ActaHortic.2012.961.30
Malavolta E (2006) Manual de nutrição mineral de plantas. São Paulo, Ceres, p 638
Minerva G, Kumar S (2012) Micropropagation of Gerbera (Gerbera jamesonii Bolus). In: Lambardi M, Ozudogru E, Jain S (eds) Protocols for micropropagation of selected economically-important horticultural plants. Methods in molecular biology (Methods and Protocols), vol 994. Humana Press, Totowa, NJ, pp 305–316
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nhut DT, Truong TTA, Nguyen TDH, Nguyen TD, Nguyen TH, Nguyen QT, Nguyen HV (2007) Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera sp. by receptacle transverse thin cell layer culture. Sci Hortic 111:146–151. https://doi.org/10.1016/j.scienta.2006.10.008
Pasqual M, Soares JDR, Rodrigues FA, Araujo AG, Santos RR (2011) Influência da qualidade de luz e silício no crescimento in vitro de orquídeas nativas e híbridas. Hortic Bras 29:324–329. https://doi.org/10.1590/S0102-05362011000300011
Reddy ASN (2001) Calcium: silver bullet in signaling. Plant Sci 161:381–404. https://doi.org/10.1016/S0168-9452(00)00386-1
Sa FP, Ledo AS, Amorim JAE, Silva AVC, Pasqual M (2016) In vitro propagation and acclimatization of genipapo accessions. Cienc Agrotec 40(2):155–163. https://doi.org/10.1590/1413-70542016402036015
Sá JF, Sampaio ES, Mendes MIS, Santos KCF, Sousa AS, Ledo CAS (2018) Culture media for the multiplication of wild Manihot species. Cienc Agrotec 42(6):598–607. https://doi.org/10.1590/1413-70542018426024718
Sahebi M, Hanafi MM, Azizi P (2016) Application of silicon in plant tissue culture. Vitro Cell Dev Biol Plant 52:226–232. https://doi.org/10.1007/s11627-016-9757-6
Schroeder JI, Allen G, Hugouvieux V, Kwak JM, Waner D (2001) Guard cell signal transduction. Annu Rev Plant Physiol Plant Mol Biol 52:627–658. https://doi.org/10.1146/annurev.arplant.52.1.627
Silva HFJ, Asmar SA, Oliveira RC, Luz JMQ, Melo B (2016) Alternative supplements and MS medium concentrations in the in vitro establishment of Dipteryx alata. Vog. Biosci J 32(5):1138–1146. https://doi.org/10.14393/BJ-v32n5a2016-33682
Silva DPC, Paiva R, Herrera RC, Silva LC, Ferreira GN, Reis MV (2018) Somatic embryogenesis of Byrsonima intermedia A. Juss.: induction and maturation via indirect approach. Plant Cell Tissue Organ Cult 133(1):115–122. https://doi.org/10.1007/s11240-017-1366-5
Sivanesan I, Jeong BR (2014) Silicon promotes adventitious shoot regeneration and enhances salinity tolerance of Ajuga multiflora Bunge by altering activity of antioxidant enzyme. Sci World J 2014:1–10. https://doi.org/10.1155/2014/521703
Soares JDR, Pasqual M, Rodrigues FA, Villa F, Carvalho JG (2008) Adubação com silício via foliar na aclimatização de um híbrido de orquídea. Cienc Agrotec 32(2):626–629. https://doi.org/10.1590/S1413-70542008000200043
Soares JDR, Pasqual M, Araujo AG, Castro EM, Pereira FJ, Braga FT (2012) Leaf anatomy of orchids micropropagated with different silicon concentrations. Acta Sci Agron 34:413–421. https://doi.org/10.4025/actasciagron.v34i4.15062
Soares JDR, Villa F, Almendagna Rodrigues F, Pasqual M (2013) Concentrações de silício e GA3 na propagação in vitro de orquídea em condição de luz natural. Sci Agrar Parana 12(4):286–292. https://doi.org/10.1818/sap.v12i4.5700
Timoteo CO, Paiva R, Reis MV, Claro PIC, Silva DPC, Marconcini JM, Oliveira JE (2019) Silver nanoparticles in the micropropagation of Campomanesia rufa (O. Berg) Nied. Plant Cell Tissue Organ Cult 137(2):359–368. https://doi.org/10.1007/s11240-019-01576-9
Vieira JGZ, Unemoto LK, Yamakami JK, Nagashima GT, Faria RT, Aguiar RS (2009) Propagação in vitro e aclimatização de um híbrido de Cattleya Lindl. (Orchidaceae) utilizando polpa de banana e água de coco. Rev Cient 37(1): 48–52. https://doi.org/10.15361/1984-5529.2009v37n1p48-52
Yoshida S, Ohnishi Y, Kitagishi K (1962) Chemical forms, mobility and deposition of silicon in rice plant. Soil Sci Plant Nutr 8(3):15–21. https://doi.org/10.1080/00380768.1962.10430992
Ziv M (2010) Silicon effects on growth acclimatization and stress tolerance of bioreactor cultured Ornithogalum dubium plants. Acta Hortic 865:29–35
Authors kindly acknowledge the National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES) and the Research Support Foundation of the State of Minas Gerais (FAPEMIG) for the financial support of this study.
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Communicated by Sergio J. Ochatt.
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da Silva, D.P.C., de Oliveira Paiva, P.D., Herrera, R.C. et al. Effectiveness of silicon sources for in vitro development of gerbera. Plant Cell Tiss Organ Cult (2020) doi:10.1007/s11240-020-01768-8
- Gerbera jamesonii
- Ornamental plants