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Overexpression of the pear PbSPMS gene in Arabidopsis thaliana increases resistance to abiotic stress

  • Xianghong Jiang
  • Junyu Zhan
  • Qi Wang
  • Xinyi Wu
  • Xiaonan Chen
  • Bing Jia
  • Pu Liu
  • Li Liu
  • Zhenfeng Ye
  • Liwu ZhuEmail author
  • Wei HengEmail author
Original Article
  • 16 Downloads

Abstract

The fruit of ‘Dangshansuli’ pear is yellowish green in colour, while that of its mutant ‘Xiusu’ is russet in colour. A differentially expressed gene, PbSPMS, was screened from the transcriptomes of the exocarps of ‘Dangshansuli’ and ‘Xiusu’ fruit. To understand the role of PbSPMS in the russet exocarp formation of ‘Xiusu’, the expression of the PbSPMS gene in the exocarp of ‘Dangshansuli’ and ‘Xiusu’ at different stages of development was analysed. The functions of the PbSPMS gene in phenolic synthesis and suberin and polyamine (PA) deposition in Arabidopsis plants and the responses to drought and salt stress were also studied. The results showed that the relative area of vascular bundles of the transgenic PbSPMS Arabidopsis plants was larger than that of the wild-type (WT) plants and that the secondary thickened cell wall of the xylem was thicker in the transgenic Arabidopsis plants than in the WT plants. The contents of PAs, phenolics and suberin in the PbSPMS Arabidopsis plants were greater than those in the WT plants. Under drought and salt stress, the introduction of PbSPMS facilitated the rapid accumulation of spermidine (Spd) and spermine (Spm) within a short period of time in A. thaliana, and the contents of Spd and Spm were significantly greater in the transgenic plants than in the WT plants, showing that PbSPMS participates in the stress response rapidly to reduce the negative effects of stress. Moreover, exogenous PbSPMS increased the contents of proline (Pro), H2O2, peroxidase (POD) and soluble sugars in the plants. With prolonged stress time, the contents of all the compounds increased gradually, and the longer the stress time was, the more significant the drought resistance of the PbSPMS transgenic plants. Overall, the results demonstrated that PbSPMS contributes to phenolic synthesis and suberin and PA deposition in Arabidopsis plants and improves plant resistance to both drought and salt stress.

Key message

An pear gene, PbSPMS might be involved in phenolic and suberin compound biosynthesis, and response to salinity and drought stress in transgenic Arabidopsis thaliana.

Keywords

Pear PbSPMS Polyamine Phenols Suberin Resistance 

Abbreviations

DAFB

Days after full bloom

PbSPMS

Pyrus bretschneideri SPMS gene

SPMS

Spermine synthase

Spd

Spermidine

Spm

Spermine

Put

Putrescine

Pro

Proline

POD

Peroxidase

PAs

Polyamines

WT

Wild-type Arabidopsis thaliana (Columbia)

Notes

Acknowledgements

This project was supported by the National Natural Science Foundation of China (31101519, 31972985) and the earmarked fund for China Agriculture Research System (CARS-29-14). The authors thank American Journal Experts for the helpful suggestions and revisions of the manuscript.

Author contributions

HW and JXH conceived and designed the study. ZJY and WQ constructed and transformed gene vectors, screened and purified transgenic Arabidopsis seeds. ZJY and JB collected fruits and prepared for RNA. WXY and CXN performed RT-qPCR. LP and YZF contributed to bioinformatic analysis. JXH contributed to the writing of the manuscript and data analysis. ZJY and ZLW revised the manuscript. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.School of HorticultureAnhui Agricultural UniversityHefeiPeople’s Republic of China

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