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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 139, Issue 3, pp 531–546 | Cite as

NaCl-induced stress: physiological responses of six halophyte species in in vitro and in vivo culture

  • Yuping Xiong
  • Hanzhi Liang
  • Haifeng Yan
  • Beiyi Guo
  • Meiyun Niu
  • Shuangyan Chen
  • Shuguang Jian
  • Hai Ren
  • Xinhua Zhang
  • Yuan Li
  • Songjun Zeng
  • Kunlin Wu
  • Feng Zheng
  • Jaime A. Teixeira da Silva
  • Guohua MaEmail author
Original Article
  • 107 Downloads

Abstract

To investigate the mechanisms underlying salt tolerance, physiological parameters of six halophyte species [Vitex rotundifolia L., Clerodendrum inerme (L.) Gaertn, Phyla nodiflora (L.) Greene, Scaevola sericea Vahl, Alternanthera bettzickiana (Regel) Nichols, and Dracaena cambodiana Pierre ex Gagn] under NaCl stress in in vitro and in vivo culture tests were examined. Membership function analysis and cluster analysis divided the six species, based on their salt tolerance level, into three groups: Group 1 (highly salt tolerant) included C. inerme, A. bettzickiana and S. sericea; Group 2 (moderately salt tolerant) included P. nodiflora; Group 3 (weakly salt tolerant) included V. rotundifolia and D. cambodiana. In response to in vitro NaCl stress, all six species showed a significant increase in the activities of antioxidant enzymes. NaCl stress enhanced free proline content in the leaves of all six species. CAT, SOD activity and proline accumulation were significantly correlated with the growth of C. inerme, P. nodiflora and A. bettzickiana under in vitro NaCl treatment. We conclude that NaCl-tolerant plants may suffer slight damage within a certain salt concentration, as evidenced by the activities of antioxidant enzymes and the accumulation of free proline.

Key message

Six halophytic species showed a different salt tolerance level under in vitro and in vivo culture tests, due to the activities of antioxidant enzymes and the accumulation of free proline.

Keywords

Halophyte Clerodendrum inerme (L.) Gaertn Vitex rotundifolia L. Phyla nodiflora (L.) Greene Scaevola sericea Vahl Alternanthera bettzickiana (Regel) Nichols Dracaena cambodiana Pierre ex Gagn Salt tolerance Physiological parameters 

Abbreviations

BA

6-Benzyladenine

CAT

Catalase

FW

Fresh weight

IBA

Indole-3-butyric acid

MDA

Malondialdehyde

MS

Murashige and Skoog

NAA

α-Naphthaleneacetic acid

POD

Peroxidase

SOD

Superoxide dismutase

RL

Root length

RP

Rooting percentage

VI

Visible injury

Notes

Acknowledgements

The authors thank Guangdong Zhongke Qilin Landscape Co., Ltd. for providing D. cambodiana plantlets.

Author contributions

YPX and HZL prepared samples for all analyses. HFY, SYC, BYG and MYN conducted the statistical analysis of physiological changes. YPX and HFY were also involved in statistical analysis and wrote the manuscript. JATdS offered interpretative analysis and co-wrote the manuscript. SGJ, HR, XHZ, YL, SJZ, KLW, FZ, JATdS and GHM designed the experiment and provided guidance for the study. All authors read and approved the manuscript.

Funding

This work was financially supported by the National Key Research and Development Program of China (2016YFC1403000/2016YFC1403002), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA13020500) and the National Science and Technology Support Program (2015BAL04B04). The funding agencies had no role in the design, analysis, and interpretation of the data or writing of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yuping Xiong
    • 1
    • 2
  • Hanzhi Liang
    • 1
    • 2
  • Haifeng Yan
    • 3
  • Beiyi Guo
    • 1
    • 2
  • Meiyun Niu
    • 1
    • 2
  • Shuangyan Chen
    • 1
  • Shuguang Jian
    • 1
  • Hai Ren
    • 1
  • Xinhua Zhang
    • 1
  • Yuan Li
    • 1
  • Songjun Zeng
    • 1
  • Kunlin Wu
    • 1
  • Feng Zheng
    • 1
  • Jaime A. Teixeira da Silva
    • 4
  • Guohua Ma
    • 1
    Email author
  1. 1.Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical GardenThe Chinese Academy of SciencesGuangzhouChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Cash Crop Institute of Guangxi Academy of Agricultural SciencesNanningChina
  4. 4.Miki-choJapan

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