Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 138, Issue 3, pp 475–488 | Cite as

Melatonin and phenolics biosynthesis-related genes in Vitis vinifera cell suspension cultures are regulated by temperature and copper stress

  • Lihua Wang
  • Mengjiao An
  • Weidong HuangEmail author
  • Jicheng ZhanEmail author
Original Article


Temperature and copper stress are two common abiotic stresses in viticulture. To better understand biosynthesis of melatonin and phenolics in response to these two abiotic stresses, cell suspension cultures of Vitis vinifera were treated with high temperature (HT, 38 °C), low temperature (LT, 16 °C) and 100 μM CuSO4 to investigate whether genes associated with the biosynthesis of melatonin (TDC, SNAT and ASMT) and phenolics (PAL, C4H, CHS, CHI and STS) were involved in response to these two abiotic stresses, as well as the effects of these two stresses on total phenolics, total flavonoids contents and oxidative stress. The expression patterns indicated that TDC, SNAT, ASMT, PAL and C4H were significantly upregulated in response to HT, while CHS and CHI first increased and then decreased, and STS was significantly downregulated. SNAT was significantly upregulated in response to LT, while TDC, ASMT, C4H and CHS first increased and then decreased, and PAL, CHI and STS were significantly downregulated. Copper treatment significantly induced the expression of TDC, SNAT and ASMT, and ASMT was the most significantly induced. C4H, CHI and STS were significantly upregulated in response to copper treatment, while PAL first increased and then decreased. Both HT and LT treatment significantly enhanced the contents of total phenolics and total flavonoids. The malondialdehyde (MDA) content of the cells treated with HT significantly increased. The treatment with CuSO4 significantly enhanced the contents of MDA and H2O2 in the cells. The results suggest that melatonin and phenolics biosynthesis-related genes were involved in HT, LT and copper stress response. The same gene showed different response patterns to the different stresses. HT and copper stress may cause oxidative stress.

Key Message

Whether the biosynthesis of melatonin and phenolics in grapes was also involved in response to temperature and copper stress will provide inspiration for the practice of stress-resistant viticulture


Cell suspension cultures Temperature Copper Melatonin Phenolics Vitis vinifera 



N-acetyl-5-hydroxytryptamine-methyl transferase


Cinnamate 4-hydroxylase




Chalcone synthase


Dry weight


Fresh weight


High temperature


Low temperature




Phenylalanine ammonia-lyase


Quantitative real-time polymerase chain reaction


Reactive oxygen species


Serotonin N-acetyltransferase


Stilbene synthase


Tryptophan decarboxylase



This study was funded by the National Natural Science Foundation of China (Grant No. 31572078 & 31471835).

Author contributions

Lihua Wang proposed the ideas and designed the experiments. Lihua Wang and Mengjiao An performed the experiments. Lihua Wang performed the data analysis and wrote the paper. Weidong Huang and Jicheng Zhan secured the funds to support this research. Jicheng Zhan revised the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human and animal participants

This article does not contain any studies with human or animal subjects.

Supplementary material

11240_2019_1643_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 18 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Beijing Key Laboratory of Viticulture and Enology, College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingPeople’s Republic of China

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