Advertisement

Effect of cytokinins and sucrose concentration on the efficiency of micropropagation of ‘Zes006’ Actinidia chinensis var. chinensis, a red-fleshed kiwifruit cultivar

  • H. Saeiahagh
  • M. Mousavi
  • Claudia Wiedow
  • H. B. Bassett
  • R. PathiranaEmail author
Original Article
  • 73 Downloads

Abstract

The effect of N6(3-hydroxybenzyl)adenine (meta-Topolin—mT) was compared with that of N6-benzylaminopurine (BAP) and zeatin at the proliferation stage of micropropagation of red-fleshed Actinidia chinensis var. chinensis ‘Zes006’ in two separate experiments. Shoot number, shoot weight, leaf number and leaf area were significantly higher in mT-supplemented media compared with BAP or zeatin. When transferred to rooting media, plantlets that were propagated in mT-supplemented media readily produced roots, enabling easy acclimation to the greenhouse, whereas none of the plantlets propagated in BAP- or zeatin-supplemented media produced roots. Using 12 pairs of Simple Sequence Repeat primers designed for A. chinensis var. chinensis, a very low rate of somaclonal variation was detected at some loci in plantlets produced in zeatin- (1.04%), BAP- (0.4%) as well as in mT- (0.2%) supplemented media. Overall, mT in equimolar concentrations was the better cytokinin for tissue culture of ‘Zes006’ kiwifruit and may well be applicable to many other kiwifruit genotypes.

Key message

Supplementation of media with meta-Topolin for in vitro propagation of the red-fleshed kiwifruit cultivar ‘Zes006’ enhanced better shoot proliferation, giving healthy plantlets that were easier to acclimatize to the greenhouse environment compared with media supplemented with 6-benzylaminopurine or zeatin. It also induced a lower rate of somaclonal variation, as detected by SSR markers.

Keywords

Shoot proliferation Acclimation Meta-Topolin Somaclonal variation SSR markers In vitro culture O-glucosylation 

Notes

Acknowledgements

We thank Andrew Mullan and Belinda Diepenheim for media preparation and Tony Corbett for figures. We thank Duncan Hedderley for comments on statistical analysis, and Drs Paul Johnston and Sue Gardiner for useful comments on the manuscript. This work was funded by The New Zealand Institute for Plant and Food Research Limited under the Kiwifruit Royalty Investment Programme.

Author Contributions

RP, HS and CW conceptualised the research and designed the experiments. HS, CW and RP conducted the research. HBB designed the primers described in Table 1. RP managed the project. HS, RP, CW and MM wrote the manuscript. HS conducted the statistical analysis.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest with the contents of this article.

Supplementary material

11240_2019_1597_MOESM1_ESM.pdf (145 kb)
Supplementary material 1 (PDF 144 KB)
11240_2019_1597_MOESM2_ESM.pdf (391 kb)
Supplementary material 2 (PDF 391 KB)

References

  1. Amoo SO, Van Staden J (2013) Influence of plant growth regulators on shoot proliferation and secondary metabolite production in micropropagated Huernia hystrix. Plant Cell Tissue Organ Cult 112(2):249–256Google Scholar
  2. Amoo SO, Finnie JF, Van Staden J (2011) The role of meta-topolins in alleviating micropropagation problems. Plant Growth Regul 63(2):197–206Google Scholar
  3. Amoo SO, Aremu AO, Van Staden J (2012) In vitro plant regeneration, secondary metabolite production and antioxidant activity of micropropagated Aloe arborescens Mill. Plant Cell Tissue Organ Cult 111(3):345–358.  https://doi.org/10.1007/s11240-012-0200-3 Google Scholar
  4. Amoo SO, Aremu AO, Moyo M, Szucova L, Dolezal K, Van Staden J (2014) Physiological effects of a novel aromatic cytokinin analogue in micropropagated Aloe arborescens and Harpagophytum procumbens. Plant Cell Tissue Organ Cult 116(1):17–26.  https://doi.org/10.1007/s11240-013-0377-0 Google Scholar
  5. Amoo SO, Aremu AO, Moyo M, Sunmonu TO, Plihalova L, Dolezal K, Van Staden J (2015) Physiological and biochemical effects of a tetrahydropyranyl-substituted meta-topolin in micropropagated Merwilla plumbea. Plant Cell Tissue Organ Cult 121(3):579–590.  https://doi.org/10.1007/s11240-015-0728-0 Google Scholar
  6. Aremu AO, Bairu MW, Dolezal K, Finnie JF, Van Staden J (2012a) Topolins: a panacea to plant tissue culture challenges? Plant Cell Tissue Organ Cult 108(1):1–16.  https://doi.org/10.1007/s11240-011-0007-7 Google Scholar
  7. Aremu AO, Bairu MW, Szucova L, Dolezal K, Finnie JF, Van Staden J (2012b) Assessment of the role of meta-topolins on in vitro produced phenolics and acclimatization competence of micropropagated ‘Williams’ banana. Acta Physiol Plant 34(6):2265–2273.  https://doi.org/10.1007/s11738-012-1027-6 Google Scholar
  8. Aremu AO, Bairu MW, Szucova L, Finnie JF, Van Staden J (2012c) The role of meta-topolins on the photosynthetic pigment profiles and foliar structures of micropropagated ‘Williams’ bananas. J Plant Physiol 169(15):1530–1541.  https://doi.org/10.1016/j.jplph.2012.06.006 Google Scholar
  9. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24(1):1Google Scholar
  10. Bachiri Y, Song GQ, Plessis P, Shoar-Ghaffari A, Rekab T, Morisset C (2001) Routine cryopreservation of kiwifruit (Actinidia spp) germplasm by encapsulation-dehydration: Importance of plant growth regulators. Cryoletters 22(1):61–74Google Scholar
  11. Bairu MW, Stirk WA, Dolezal K, Van Staden J (2007) Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell. Tissue Organ Cult 90(1):15–23Google Scholar
  12. Bairu MW, Stirk WA, Doležal K, van Staden J (2008) The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’(Musa spp. AAA). Plant Cell Tissue Organ Cult 95(3):373–379Google Scholar
  13. Biswas MK, Dutt M, Roy UK, Islam R, Hossain M (2009) Development and evaluation of in vitro somaclonal variation in strawberry for improved horticultural traits. Sci Hortic 122(3):409–416.  https://doi.org/10.1016/j.scienta.2009.06.002 Google Scholar
  14. Chauhan RD, Taylor NJ (2018) Meta-topolin stimulates de novo shoot organogenesis and plant regeneration in cassava. Plant Cell Tiss Organ Cult (PCTOC) 132(1):219–224Google Scholar
  15. Cline MG (1991) Apical dominance. Bot Rev 57(4):318–358Google Scholar
  16. Debenham MC, Seelye JF, Mullan AC (2016) An in vitro repository for clonal kiwifruit. Acta Hort 1113:93–97.  https://doi.org/10.17660/ActaHortic.2016.1113.13 Google Scholar
  17. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  18. Fraser LG, Tsang GK, Datson PM, De Silva HN, Harvey CF, Gill GP, Crowhurst RN, McNeilage MA (2009) A gene-rich linkage map in the dioecious species Actinidia chinensis (kiwifruit) reveals putative X/Y sex-determining chromosomes. BMC Genom 10(1):102.  https://doi.org/10.1186/1471-2164-10-102 Google Scholar
  19. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50(1):151–158.  https://doi.org/10.1016/0014-4827(68)90403-5 Google Scholar
  20. Gao D-Y, Vallejo VA, He B, Gai Y-C, Sun L-H (2009) Detection of DNA changes in somaclonal mutants of rice using SSR markers and transposon display. Plant Cell Tissue Organ Cult 98(2):187–196Google Scholar
  21. Gentile A, Gutierrez MJ, Martinez J, Frattarelli A, Nota P, Caboni E (2014) Effect of meta-Topolin on micropropagation and adventitious shoot regeneration in Prunus rootstocks. Plant Cell Tissue Organ Cult 118(3):373–381.  https://doi.org/10.1007/s11240-014-0489-1 Google Scholar
  22. Gentile A, Frattarelli A, Nota P, Condello E, Caboni E (2017) The aromatic cytokinin meta-topolin promotes in vitro propagation, shoot quality and micrografting in Corylus colurna L. Plant Cell Tissue Organ Cult 128(3):693–703.  https://doi.org/10.1007/s11240-016-1150-y Google Scholar
  23. Han ML, Gleave AP, Wang TC (2010) Efficient transformation of Actinidia arguta by reducing the strength of basal salts in the medium to alleviate callus browning. Plant Biotechnol Rep 4(2):129–138.  https://doi.org/10.1007/s11816-010-0128-1 Google Scholar
  24. Harding K, Lynch PT, Johnston JW (2009) Epigenetic changes associated with the cryopreservation of clonal crops. Cryoletters 30(5):390–391Google Scholar
  25. Ivanova M, van Staden J (2008) Effect of ammonium ions and cytokinins on hyperhydricity and multiplication rate of in vitro regenerated shoots of Aloe polyphylla. Plant Cell Tissue Organ Cult 92(2):227–231.  https://doi.org/10.1007/s11240-007-9311-7 Google Scholar
  26. Lata H, Chandra S, Techen N, Khan IA, ElSohly MA (2016) In vitro mass propagation of Cannabis sativa L.: a protocol refinement using novel aromatic cytokinin meta-topolin and the assessment of eco-physiological, biochemical and genetic fidelity of micropropagated plants. J Appl Res Med Aromat Plants 3(1):18–26.  https://doi.org/10.1016/j.jarmap.2015.12.001 Google Scholar
  27. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18(1):100–106Google Scholar
  28. Mathew L, McLachlan A, Jibran R, Burritt DJ, Pathirana R (2018) Cold, antioxidant and osmotic pre-treatments maintain the structural integrity of meristematic cells and improve plant regeneration in cryopreserved kiwifruit shoot tips. Protoplasma 255(4):1065–1077.  https://doi.org/10.1007/s00709-018-1215-3 Google Scholar
  29. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  30. Naidoo D, Aremu AO, Van Staden J, Finnie JF (2017) In vitro plant regeneration and alleviation of physiological disorders in Scadoxus puniceus. South Afr J Bot 109:316–322.  https://doi.org/10.1016/j.sajb.2017.01.010 Google Scholar
  31. Palombi M, Damiano C (2002) Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev). Plant Cell Rep 20(11):1061–1066.  https://doi.org/10.1007/s00299-001-0430-z Google Scholar
  32. Pandey R, Singh S, Rastogi J, Sharma M, Singh R (2012) Early assessment of genetic fidelity in sugarcane (Saccharum officinarum) plantlets regenerated through direct organogenesis with RAPD and SSR markers. Aust J Crop Sci 6(4):618Google Scholar
  33. Pathirana R, Deroles S, Hoeata K, Montefiori M, Tyson J, Wang T, Datson PM, Hellens RP (2016) Fast-tracking kiwifruit breeding through mutagenesis. Acta Hort 1127:217–222.  https://doi.org/10.17660/ActaHortic.2016.1127.34 Google Scholar
  34. Prado MJ, Gonzalez MV, Romo S, Herrera MT (2007) Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation. Plant Cell Tiss Organ Cult 88(1):1–10Google Scholar
  35. Sakakibara H (2006) Cytokinins: activity, biosynthesis, and translocation. Annu Rev Plant Biol 57:431–449.  https://doi.org/10.1146/annurev.arplant.57.032905.105231 Google Scholar
  36. Sato M, Hosokawa M, Doi M (2011) Somaclonal variation is induced de novo via the tissue culture process: a study quantifying mutated cells in Saintpaulia. PLoS ONE 6(8):e23541Google Scholar
  37. Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9(7):671Google Scholar
  38. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234Google Scholar
  39. Shimizu-Sato S, Tanaka M, Mori H (2009) Auxin–cytokinin interactions in the control of shoot branching. Plant Mol Biol 69(4):429Google Scholar
  40. Skirvin RM, McPheeters KD, Norton M (1994) Sources and frequency of somaclonal variation. HortScience 29(11):1232–1237Google Scholar
  41. Smulders MJM, De Klerk GJ (2011) Epigenetics in plant tissue culture. Plant Growth Regul 63(2):137–146Google Scholar
  42. Strnad M, Hanuš J, Vaněk T, Kamínek M, Ballantine JA, Fussell B, Hanke DE (1997) Meta-topolin, a highly active aromatic cytokinin from poplar leaves (Populus × canadensis Moench., cv. Robusta). Phytochemistry 45(2):213–218.  https://doi.org/10.1016/S0031-9422(96)00816-3 Google Scholar
  43. Takahashi W, Sugawara F, Yamamoto N, Bando E, Matsushita J, Tanaka O (2004) Plant regeneration in Actinidia polygama Miq. by leaf, stem, and petiole culture with zeatin, and from stem-derived calli on low-sucrose medium. J For Res 9(1):85–88.  https://doi.org/10.1007/s10310-003-0053-z Google Scholar
  44. Teklehaymanot T, Wannakrairoj S, Pipattanawong N (2010) Meta-topolin for pineapple shoot multiplication under three in vitro systems. Am-Eur J Agric Environ Sci 7(2):157–162Google Scholar
  45. Tyson JL, Vergara MJ, Butler RC, Seelye JF, Morgan ER (2018) Survival, growth and detection of pv. in cultures. N Z J Crop Hortic Sci 46(4):319–333Google Scholar
  46. Wang TC, Ran YD, Atkinson RG, Gleave AP, Cohen D (2006) Transformation of Actinidia eriantha: A potential species for functional genomics studies in Actinidia. Plant Cell Rep 25(5):425–431.  https://doi.org/10.1007/s00299-005-0080-7 Google Scholar
  47. Werbrouck SP, Strnad M, Van Onckelen HA, Debergh PC (1996) Meta-topolin, an alternative to benzyladenine in tissue culture? Physiol Plant 98(2):291–297Google Scholar
  48. Westhuizen AVD (2014) Use of meta-topolin as an alternative cytokinin in the tissue culture of Eucalyptus species. Acta Horticult:25–28Google Scholar
  49. Wu J-H, Ferguson AR, Murray BG (2011) Manipulation of ploidy for kiwifruit breeding: in vitro chromosome doubling in diploid Actinidia chinensis Planch. Plant Cell Tissue Organ Cult 106(3):503–511.  https://doi.org/10.1007/s11240-011-9949-z Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.The New Zealand Institute for Plant and Food Research LimitedPalmerston NorthNew Zealand
  2. 2.Department of Horticultural Science, Faculty of AgricultureShahid Chamran University of AhvazAhvazIran

Personalised recommendations