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Synergistic effect of coronatine and sorbitol on artemisinin production in cell suspension culture of Artemisia annua L. cv. Anamed

  • Maryam SalehiEmail author
  • Ghasem Karimzadeh
  • Mohammad Reza Naghavi
Original Article
  • 54 Downloads

Abstract

Artemisinin is an efficient anti-malarial drug and it possesses biological activity against a wide range of cancers. The combined application of two different elicitors can be an efficient way to increase the production of secondary metabolite in plant cell cultures. The results of coronatine (Cor) pretreatment and three concentrations of sorbitol were assessed on the growth, biochemical traits, expression of artemisinin biosynthetic genes, and artemisinin production in Artemisia annua cell suspension culture (CSC). After pretreating CSC with 0.05 µM Cor [on the 14th day (three days before the stationary phase) for 48 h], liquid medium in the culture flasks was decanted and replaced with fresh medium (containing 30 g/L sucrose) plus or minus sorbitol at selected concentrations (0, 20, 30, and 40 g/L) on day 16th (one day before the stationary phase). The sorbitol treatment enhanced the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) and resulted in oxidative stress. Cor-pretreatment increased the activity of antioxidant enzymes and consequently it reduced H2O2 content and oxidative stress which resulted in decreased MDA content and better growth. The application of Cor plus sorbitol resulted in a dramatic enhancement in the expression of artemisinin biosynthetic genes and artemisinin production at all concentrations. The expression levels of artemisinin biosynthetic genes (about 7.66, 8.67, 8.67, and 8.33-fold in ADS, CYP71AV1, ALDH1, and DBR2 genes, respectively at 4 h after sorbitol treatment) and artemisinin production (9.33 mg/L, 8-fold) peaked at 30 g/L sorbitol plus Cor and decreased at 40 g/L sorbitol, probably because of higher oxidative stress.

Keymessage

The simultaneous application of Cor and sorbitol resulted in a dramatic enhancement in the expression of artemisinin biosynthetic genes and artemisinin production owing to a synergistic or potentiating result.

Keywords

Antioxidant enzyme Osmotic stress Relative gene expression Secondary metabolite 

Abbreviations

AA

Artemisinic aldehyde

ADS

Amorpha-4, 11-diene synthase

ALDH1

Aldehyde dehydrogenase 1

APX

Ascorbate peroxidase

Cor

Coronatine

CSC

Cell suspension culture

CYP71AV1

Amorphadiene-12-hydroxylase

DBR2

Artemisinic aldehyde Δ11(13) reductase

DCW

Dry cell weight

DHAA

Dihydroartemisinic aldehyde

EDTA

Ethylenediaminetetracetic acid

GA3

Gibberellic acid

GR

Glutathione reductase

H2O2

Hydrogen peroxide

HPLC

High-performance liquid chromatography

Kin

Kinetin

LSD

Least significant difference

MDA

Malondialdehyde

MeJA

Methyl jasmonate

NAA

1-Naphthaleneacetic acid

PVP-40

Polyvinylpyrrolidone

RED1

Dihydroartemisinic aldehyde reductase

ROS

Reactive oxygen species

SE

Standard error

SM

Secondary metabolite

SOD

Superoxide dismutase

TBA

Thiobarbituric acid

TCA

Trichloroacetic acid

Notes

Acknowledgements

Authors gratefully acknowledge the support provided for this survey by the Tarbiat Modares University, Tehran, Iran.

Author Contributions

Maryam Salehi designed and performed experiments and prepared the manuscript under the joint supervision of Assoc. prof. G. Karimzadeh and Prof. M. R. Naghavi. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

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© Springer Nature B.V. 2019

Authors and Affiliations

  • Maryam Salehi
    • 1
    Email author
  • Ghasem Karimzadeh
    • 1
  • Mohammad Reza Naghavi
    • 2
  1. 1.Department of Plant Genetics and Breeding, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Department of Agronomy and Plant Breeding, College of Agricultural and Natural ResourcesUniversity of TehranKarajIran

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