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Silver nanoparticles in the micropropagation of Campomanesia rufa (O. Berg) Nied

  • Caroline de Oliveira TimoteoEmail author
  • Renato Paiva
  • Michele Valquíria dos Reis
  • Pedro Ivo Cunha Claro
  • Diogo Pedrosa Corrêa da Silva
  • Jose Manoel Marconcini
  • Juliano Elvis de Oliveira
Original Article

Abstract

With the advancement of nanotechnology, nanomaterials are beginning to be employed in different areas of science, including plant tissue culture. Among nanomaterials, silver nanoparticles (AgNPs) are widely used because of their antibacterial effects. However, knowledge about the effects of AgNPs on in vitro cultivation of plant species is poor. The present study aims to analyse the effects of AgNPs on the in vitro propagation of Campomanesia rufa. Nodal segments were sectioned and cultured in MS medium to induce buds. The MS medium was supplemented with benzylaminopurine, different concentrations of AgNPs or AgNO3. AgNPs were synthesized and characterized as a function of time. The shoots were analysed for number, height and fresh weight. Biochemical and light microscopy and scanning electron microscopy were also performed. Data from the characterization of AgNPs demonstrate that the heating process for sterilization of the culture medium promotes an agglomeration of AgNPs. The lowest concentrations AgNPs (0.385, 0.77 and 1.54 mg L−1) did not affect the in vitro multiplication, since no significant differences were observed in relation to the control, as for the number, height and fresh weight of the shoots formed, however, in the treatments with 15.4 mg L−1 AgNPs and AgNO3, a reduction in the number of shoots was observed. No biochemical, morphological or anatomical changes were observed in the shoots formed. Concludes, AgNPs did not affect the in vitro multiplication at low concentrations but may cause more damage to plant development than the use of AgNO3, depending on the concentration used.

Key message

This manuscript reports that silver nanoparticles not affect the in vitro multiplication of Campomanesia rufa, and the results are important because explain the behavior of AgNPs under high temperatures.

Keywords

AgNPs Nanotechnology Gabiroba Tissue culture 

Notes

Acknowledgements

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. Sincere thanks to the Foundation for Research Support of the State of Minas Gerais (FAPEMIG) and the National Council for Scientific and Technological Development (CNPq), for financial support. We are also grateful to the National Laboratory of Nanotechnology for Agribusiness - Embrapa Instrumentation - São Carlos-SP, where the silver nanoparticles were characterized.

Author contributions

CT, MR planned the study, performed the experiments, analyzed the results and wrote the manuscript. RP research supervisor, contributed with scientific advice, corrected and revised the final version of the manuscript. JO, JM, PC and DS assisted in the planning and supervision of the study, performed the experiments, analyzed the results and corrected the manuscript. PC assisted performed experiments and analyzed the results. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors state that the present study was conducted in the absence of commercial or financial relationships that could result in a potential conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Caroline de Oliveira Timoteo
    • 1
    Email author
  • Renato Paiva
    • 1
  • Michele Valquíria dos Reis
    • 1
  • Pedro Ivo Cunha Claro
    • 2
  • Diogo Pedrosa Corrêa da Silva
    • 3
  • Jose Manoel Marconcini
    • 4
  • Juliano Elvis de Oliveira
    • 5
  1. 1.Department of Biology, Sector of Plant PhysiologyFederal University of LavrasLavrasBrazil
  2. 2.Graduate Program in Materials Science and Engineering (PPG-CEM)Federal University of São CarlosSão CarlosBrazil
  3. 3.Department of AgricultureFederal University of LavrasLavrasBrazil
  4. 4.National Nanotechnology Laboratory for Agribusiness (LNNA)Embrapa InstrumentationSão CarlosBrazil
  5. 5.Department of EngineeringFederal University of LavrasLavrasBrazil

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