Effect of sucrose on physiological and biochemical changes of proliferated shoots of Bambusa vulgaris Schrad. Ex Wendl in temporary immersion

  • Yudith García-RamírezEmail author
  • Gloria Patricia Barrera
  • Marisol Freire-Seijo
  • Raúl Barbón
  • Mairenys Concepción-Hernández
  • Milady F. Mendoza-Rodríguez
  • Sinesio Torres-García
Original Article


Tissue culture systems have contributed to the massive propagation of bamboos. However, micro-propagated plants show a low percentage of rooting and ex vitro survival due to anatomical and physiological anomalies induced by in vitro factors. This study aims to determine the effect of sucrose on physiological and biochemical changes in Bambusa vulgaris Schrad. Ex Wendl shoots cultured in a temporary immersion system and in plantlets growing under greenhouse conditions. For this purpose, two concentrations of sucrose (20 and 30 g L−1) and a control treatment (sucrose-free medium) were used in a rooting culture medium. Our data revealed that the absence of sucrose in the culture medium improved the morpho-physiological, biochemical and anatomical development of the shoots. However, a high sucrose concentration decreased the total chlorophyll content and leaf area in shoots. Additionally, the hydrogen peroxide content and malondialdehyde increased in shoots cultured with 30 g L−1 of sucrose. On the other hand, scanning electron microscopy revealed that leaves of shoots which grow on medium containing 30 g L−1 sucrose displayed anatomical changes in the stomata of plants, whereas those from sucrose-free medium exhibited normal structural development. Finally, shoots cultured without sucrose showed a high survival rate and allowed a better adaptation of plantlets in the greenhouse. The temporary immersion systems combined with culture medium without sucrose offer new prospects for improving the physiology and biochemistry of plantlets during in vitro to ex vitro transition.


Bamboo In vitro propagation Liquid medium Oxidative stress Scanning electron microscopy 



This research was supported by the International project EDUNABIO—Educational Network in Agrobiodiversity. We would like to express particular thanks to EDUNABIO for financial support and a scholarship, for developing this research.

Author contributions

This statement is to certify that all authors have seen and approved the manuscript being submitted. The presented idea was conceived by YG-R with support from STG and MM-R. All authors discussed the results and contributed to the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yudith García-Ramírez
    • 1
    Email author
  • Gloria Patricia Barrera
    • 2
  • Marisol Freire-Seijo
    • 1
  • Raúl Barbón
    • 1
  • Mairenys Concepción-Hernández
    • 1
  • Milady F. Mendoza-Rodríguez
    • 1
  • Sinesio Torres-García
    • 3
  1. 1.Instituto de Biotecnología de las PlantasUniversidad Central “Marta Abreu” de Las VillasSanta ClaraCuba
  2. 2.Corporación Colombiana de Investigación AgropecuariaBogotáColombia
  3. 3.Facultad de Ciencias AgropecuariasUniversidad Central “Marta Abreu” de Las VillasSanta ClaraCuba

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