Isolation and functional identification of an apple MdCER1 gene
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The outermost layer of the plant epidermis is covered by cuticular wax, which resists UV radiation, insects, and pathogens, and protects the plant from various environmental stressors. We cloned MdCER1 to further study the molecular regulatory pathway of cuticular wax in apple (Malus × domestica Borkh.). The results showed that MdCER1 is a homolog of Arabidopsis CER1, which is essential for wax synthesis. A subcellular localization study revealed that MdCER1 was localized on the endoplasmic reticulum. We examined the expression pattern of MdCER1 in different tissues and found that it was constitutively expressed in roots, stems, leaves, flowers, and fruits, with the highest expression in leaves. Ectopic expression of MdCER1 promoted the accumulation of cuticular wax by changing the permeability of the epidermis and the response to abscisic acid, as well as by improving drought resistance in Arabidopsis.
KeywordsApple MdCER1 Cuticular wax Functional characterization
Acyl carrier protein
Fatty acyl-coenzyme A reductase
Long-chain acyl-CoA synthetase
3-Ketoacyl coenzyme A synthase
Quantitative real-time PCR
Very long chain fatty acid
Wax crystal-sparse leaf 4
Scanning electron microscopy
This study was financially supported by the National Natural Science Foundation of China (31772275) and the Natural Science Fund for Excellent Young Scholars of Shandong Province (ZR2018JL014).
Y-JH and Y-YL planned and designed the research. C-HQ, X-YZ, HJ and H-TL performed the experiments and analyzed the data. C-HQ, and Y-YL wrote the manuscript.
Compliance with ethical standards
Conflict of interest
All the authors have declared that this article would have no conflict of interest.
Research involving human participants and/or animals
All the authors have declared our research would not involve any human participants and/or animals.
- An JP, Li R, Qu FJ, You CX, Wang XF, Hao YJ (2017) Ectopic expression of an apple cytochrome P450 gene MdCYPM1 negatively regulates plant photomorphogenesis and stress response in Arabidopsis. Biochem Biophys Res Commun 483:1–9. https://doi.org/10.1016/j.bbrc.2017.01.026 CrossRefPubMedGoogle Scholar
- Bourdenx B, Bernard A, Domergue F, Pascal S, Léger A, Roby D, Pervent M, Vile D, Haslam RP, Napier JA, Lessire R, Joubes J (2011) Overexpression of Arabidopsis CER1 promotes wax VLC-alkane biosynthesis and influences plant response to biotic and abiotic stresses. Plant Physiol. https://doi.org/10.1104/pp.111.17232 CrossRefPubMedPubMedCentralGoogle Scholar
- Koornneef M, Hanhart CJ, Thiel F (1989) A genetic and phenotypic description of eceriferum (cer) mutants in Arabidopsis thaliana. J Hered 80:118–122. https://doi.org/10.1093/oxfordjournals.jhered.a110808 CrossRefGoogle Scholar
- Liu S, Li M, Su L, Ge K, Li L, Li X, Liu X, Li L (2016) Negative feedback regulation of ABA biosynthesis in peanut (Arachis hypogaea): a transcription factor complex inhibits AhNCED1 expression during water stress. Sci Rep 6:37943. https://doi.org/10.1038/srep37943 CrossRefPubMedPubMedCentralGoogle Scholar
- Lü S, Song T, Kosma DK, Parsons EP, Rowland O, Jenks MA (2009) Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis. Plant J 59:553–564. https://doi.org/10.1111/j.1365-313X.2009.03892.x CrossRefPubMedGoogle Scholar
- Lü S, Zhao H, Des Marais DL, Parsons EP, Wen X, Xu X, Bangarusamy DK, Wang G, Rowland O, Juenger T, Bressan RA, Jenks MA (2012) Arabidopsis ECERIFERUM9 involvement in cuticle formation and maintenance of plant water status. Plant Physiol 159:930–944. https://doi.org/10.1104/pp.112.198697 CrossRefPubMedPubMedCentralGoogle Scholar
- Oshima Y, Shikata M, Koyama T, Ohtsubo N, Mitsuda N, Ohme-Takagi M (2013) MIXTA-like transcription factors and WAX INDUCER1/SHINE1 coordinately regulate cuticle development in Arabidopsis and Torenia fournieri. Plant Cell 25:1609–1624. https://doi.org/10.1105/tpc.113.110783 CrossRefPubMedPubMedCentralGoogle Scholar
- Samuels L, Kunst L, Jetter R (2008) Sealing plant surfaces: cuticular wax formation by epidermal cells. Annu Rev Plant Biol 59:683–707. https://doi.org/10.1146/annurev.arplant.59.103006.093219 CrossRefPubMedGoogle Scholar
- Wang Z, Tian X, Zhao Q, Liu Z, Li X, Ren Y, Tang J, Fang J, Xu Q, Bu Q (2018) The E3 ligase DROUGHT HYPERSENSITIVE negatively regulates cuticular wax biosynthesis by promoting the degradation of transcription factor ROC4 in rice. Plant Cell 30:228–244. https://doi.org/10.1105/tpc.17.00823 CrossRefPubMedGoogle Scholar