Isolation and functional identification of an apple MdCER1 gene
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The outermost layer of the plant epidermis is covered by cuticular wax, which resists UV radiation, insects, and pathogens, and protects the plant from various environmental stressors. We cloned MdCER1 to further study the molecular regulatory pathway of cuticular wax in apple (Malus × domestica Borkh.). The results showed that MdCER1 is a homolog of Arabidopsis CER1, which is essential for wax synthesis. A subcellular localization study revealed that MdCER1 was localized on the endoplasmic reticulum. We examined the expression pattern of MdCER1 in different tissues and found that it was constitutively expressed in roots, stems, leaves, flowers, and fruits, with the highest expression in leaves. Ectopic expression of MdCER1 promoted the accumulation of cuticular wax by changing the permeability of the epidermis and the response to abscisic acid, as well as by improving drought resistance in Arabidopsis.
KeywordsApple MdCER1 Cuticular wax Functional characterization
Acyl carrier protein
Fatty acyl-coenzyme A reductase
Long-chain acyl-CoA synthetase
3-Ketoacyl coenzyme A synthase
Quantitative real-time PCR
Very long chain fatty acid
Wax crystal-sparse leaf 4
Scanning electron microscopy
This study was financially supported by the National Natural Science Foundation of China (31772275) and the Natural Science Fund for Excellent Young Scholars of Shandong Province (ZR2018JL014).
Y-JH and Y-YL planned and designed the research. C-HQ, X-YZ, HJ and H-TL performed the experiments and analyzed the data. C-HQ, and Y-YL wrote the manuscript.
Compliance with ethical standards
Conflict of interest
All the authors have declared that this article would have no conflict of interest.
Research involving human participants and/or animals
All the authors have declared our research would not involve any human participants and/or animals.
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