Morphogenetic potential of different sources of explants for efficient in vitro regeneration of Genipa sp.
- 99 Downloads
Genipa americana L. is a species with high economic potential with considerable promise in the fruit, medicinal and agroindustrial fields. The in vitro cultivation of genipapo is an alternative that may overcome the difficulties imposed by the traditional propagation system, allowing the multiplication of plants on a large scale and using technologies in conservation programs and to improve phytopharmaceutical production. In this context, the aim of this work was to evaluate the morphogenetic potentials of different sources of explants to establish an efficient system for in vitro regeneration of G. americana. For callus induction, shoots differentiation and further plant regeneration, segments of hypocotyl, root and leaf from in vitro established seedlings were used. The explants were inoculated in MS medium supplemented with 6-benzylaminopurine (BAP) at concentrations of 0.0, 1.12, 2.25 and 3.37 mg L−1. The morphogenetic pattern and regeneration capacity showed correlations with the explant source and BAP concentration. MS medium supplemented with 1.12 mg L−1 BAP proved to be optimum for adventitious shoots induction in segments hypocotyl. It was possible to obtain a efficient protocol for the in vitro regeneration of G. americana that allowed high shoot regeneration rates (80%) using hypocotyl segments with low concentrations of BAP (1.12 mg L−1). The regenerated plantlets showed a high capacity for acclimatization, presenting 90% survival rate 30 days after exposure to the ex vitro conditions.
KeywordsRubiaceae Micropropagation Woody plants Growth regulators Genipapo
The authors are thankful to the National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES) and Foundation for Supporting Research of the State of Minas Gerais (FAPEMIG) for granting scholarships.
RRdS, PDdOP and RP conceived and designed the experiment. RRdS, RRdS, MVdR and DPCdS performed the experiments, and analyzed and interpreted the data. RRdS and PDdOP wrote the manuscript. All authors, read and approved the final version of the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Asakura I, Hoshino Y (2017) Evaluation of plant regeneration ability of different explants and establishment of an efficient regeneration system using immature seeds in Actinidia kolomikta, a cold-hardy kiwifruit relative. Sci Hortic 220:275–282. https://doi.org/10.1016/j.scienta.2017.04.012 CrossRefGoogle Scholar
- Bhuvaneshwari K, Gokulanathan A, Jayanthi M, Govindasamy V, Milella L, Lee S, Yang DC, Girija S (2016) Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites? Food Chem 194:55–60. https://doi.org/10.1016/j.foodchem.2015.07.136 CrossRefPubMedGoogle Scholar
- FAO (2017) The future of food and agriculture: trend and challenges. Rome. https://reliefweb.int/sites/reliefweb.int/files/resources/a-i6583e.pdf. Accessed 16 Mar 2018
- Joint FAO, WHO (2017) Evaluation of certain food additives. Eighty-fourth report of the joint. Expert committiee on food additives. http://apps.who.int/iris/bitstream/10665/2594 83/1/9789241210164-eng.pdf
- Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings of International Plant Propagators’ Society, vol 30, pp 421–427Google Scholar
- Mignolli F, Mariotti L, Picciarelli P, Vidoz ML (2017) Differential auxin transport and accumulation in the stem base lead toprofuse adventitious root primordia formation in the aerial roots (aer) mutant of tomato (Solanum lycopersicum L.). J Plant Physiol 213:55–65. https://doi.org/10.1016/j.jplph.2017.02.010 CrossRefPubMedGoogle Scholar
- Mikuła A, Pozoga M, Grzyb M, Rybczyn JJ (2015) An unique system of somatic embryogenesis in the tree fern Cyathea delgadii Sternb.: the importance of explant type, and physical and chemical factors. Plant Cell Tissue Organ Cult 123:467–478. https://doi.org/10.1007/s11240-015-0850-z CrossRefGoogle Scholar
- Ministério do meio ambiente MMA (2016) Espécies nativas da flora brasileira de valor econômico atual e potencial: plantas para o futuro-Região Centro-Oeste. Brasília-DF. https://www.embrapa.br/busca-de-publicacoes/-/publicacao/1073295/especies-nativas-da-flora-brasileira-de-valor-economico-atual-ou-potencial-plantas-para-o-futuro-regiao-centro-oeste. Accessed 12 Feb 2018
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
- Perez-Jimenez M, Cantero-Navarro E, Perez-Alfocea F, Cos-Terrer J (2014) Endogenous hormones response to cytokinins with regard to organogenesis in explants of peach (Prunuspersica L. Batsch) cultivars and rootstocks (P. pérsica x Prunus dulcis). Plant Physiol Biochem 84:197–202. https://doi.org/10.1016/j.plaphy.2014.09.014 CrossRefPubMedGoogle Scholar
- Rocha DI, Vieira LM, Tanaka FAO, Silva LC, Otoni WC (2012) Anatomical and ultrastructural analyses of in vitro organogenesis from root explants of commercial passion fruit (Passiflora edulis Sims). Plant Cell Tissue Organ Cult 111:69–78. https://doi.org/10.1007/s11240-012-0171-4 CrossRefGoogle Scholar
- Santana KB, Almeida AF, Souza VL, Mangabeira PAO, Silva DC, Gomes FP, Dutruch L, Loguercio LL (2012) Physiological analyses of Genipa americana L. reveals a tree with ability as phytostabilizer and rhizofilterer of chromium ions for phytoremediation of polluted watersheds. Environ Exp Bot 80:35–42. https://doi.org/10.1016/j.envexpbot.2012.02.004 CrossRefGoogle Scholar
- Simão MJ, Fonseca E, Mansur E, Pacheco G (2016) Effects of auxins and different culture systems on the adventitious root development of Passiflora pohlii Mast. and their ability to produce antioxidant compounds. Plant Cell Tissue Organ Cult 124:419–430. https://doi.org/10.1007/s11240-015-0904-2 CrossRefGoogle Scholar
- Yee TF, Goh CI, Rao AN (2010) In vitro studies on Genipa americana. J Trop Med Plants 11:71–88Google Scholar