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Enhanced accumulation of high-value deoxymiroestrol and isoflavonoids using hairy root as a sustainable source of Pueraria candollei var. mirifica

  • Orapin Udomsin
  • Gorawit Yusakul
  • Witsarut Kraithong
  • Latiporn Udomsuk
  • Tharita Kitisripanya
  • Thaweesak Juengwatanatrakul
  • Waraporn Putalun
Original Article
  • 89 Downloads

Abstract

Pueraria candollei var. mirifica (PM), a rejuvenating herb of Thailand, has been applied efficaciously to relieve symptoms of estrogen deficiency in various clinical trials. However, there is a shortage of the natural source of the plant because of high demand. A clone of hairy root was produced by genetic transformation of T-DNA from an Agrobacterium rhizogenes ATCC15834 strain. An in vitro elicitation strategy for PM hairy root was successfully established. The hairy root of PM produced a high amount of deoxymiroestrol [71.9 ± 4.67–77.9 ± 6.63 µg g−1 dry weight (DW)] and isoflavonoids (6.57 ± 0.044–7.05 ± 0.534 mg g−1 DW). These amounts are higher than that obtained from the root of its parental intact PM, which contained deoxymiroestrol (15.41 ± 0.71 µg g−1 DW) and isoflavonoids (3.45 ± 0.21 mg g−1 DW). Interestingly, the treatment of the hairy root with methyl jasmonate (50–200 µM) increased the accumulation of deoxymiroestrol (113 ± 11.4–245 ± 12.0 µg g−1 dry weight) and isoflavonoids (9.94 ± 0.365–16.49 ± 0.466 mg g−1 DW). Therefore, this approach markedly improved the production of PM phytoestrogens. The field cultivation of PM takes at least 1 year to produce a substantial amount of deoxymiroestrol and isoflavonoids; moreover, there are geographical and seasonal variations in phytoestrogen content, influencing the limited supply and quality of PM materials. Therefore, this system of PM hairy root culture and elicitation is quite simple and effective for the production of useful phytoestrogens for phytochemical and cosmeceutical industries. Additionally, this process can be scaled up to the industrial level as a sustainable source of PM.

Keywords

Pueraria candollei var. mirifica Elicitation Phytoestrogen Deoxymiroestrol Isoflavonoids 

Notes

Acknowledgements

The authors thank the Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand for their support. The authors thank Dr. Chaiyo Chaichantipyuth, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand for providing deoxymiroestrol and kwakhurin.

Author contributions

The experiments were designed and performed by OU. Data analysis, interpretation, and manuscript preparation were performed by OU and GY. WK performed experiments in the part of an untransformed root culture of PM. The molecular marker confirmation of hairy root genetic transformation was performed by LU. TK and TJ provided advice and technical support for plant tissue culture and HPLC analysis. Grant and research facilities were managed by WP. All authors were involved in the editing of all versions for publication.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Anukunwithaya T, Poo P, Hunsakunachai N, Rodsiri R, Malaivijitnond S, Khemawoot P (2018) Absolute oral bioavailability and disposition kinetics of puerarin in female rats. BMC Pharmacol Toxicol 19(1):25.  https://doi.org/10.1186/s40360-018-0216-3 CrossRefPubMedPubMedCentralGoogle Scholar
  2. Boonsnongcheep P, Korsangruang S, Soonthornchareonnon N, Chintapakorn Y, Saralamp P, Prathanturarug S (2010) Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tissue Org 101(2):119–126.  https://doi.org/10.1007/s11240-010-9668-x CrossRefGoogle Scholar
  3. Chandeying V, Lamlertkittikul S (2007) Challenges in the conduct of Thai herbal scientific study: efficacy and safety of phytoestrogen, Pueraria mirifica (Kwao Keur Kao), phase I, in the alleviation of climacteric symptoms in perimenopausal women. J Med Assoc Thail 90(7):1274–1280Google Scholar
  4. Chandeying V, Sangthawan M (2007) Efficacy comparison of Pueraria mirifica (PM) against conjugated equine estrogen (CEE) with/without medroxyprogesterone acetate (MPA) in the treatment of climacteric symptoms in perimenopausal women: phase III study. J Med Assoc Thail 90(9):1720–1726Google Scholar
  5. Chansakaow S, Ishikawa T, Seki H, Sekine K, Okada M, Chaichantipyuth C (2000) Identification of deoxymiroestrol as the actual rejuvenating principle of “Kwao Keur”, Pueraria mirifica. The known miroestrol may be an artifact. J Nat Prod 63(2):173–175CrossRefGoogle Scholar
  6. Chen H, Seguin P, Archainbault A, Constan L, Jabaji S (2009) Gene expression and isoflavone concentrations in soybean sprouts treated with chitosan. Crop Sci 49(1):224–236.  https://doi.org/10.2135/cropsci2007.09.0536 CrossRefGoogle Scholar
  7. Cherdshewasart W, Sriwatcharakul S (2007) Major isoflavonoid contents of the 1-year-cultivated phytoestrogen-rich herb, Pueraria mirifica. Biosci Biotechnol Biochem 71(10):2527–2533.  https://doi.org/10.1271/bbb.70316 CrossRefPubMedGoogle Scholar
  8. Cho JG, Park HJ, Huh GW, Lee S, Bang MH, Choi KS, Oh CH, Ko SK, Cho SY, Chai KY, Kim JH, Baek NI (2014) Flavonoids from Pueraria mirifica roots and quantitative analysis using HPLC. Food Sci Biotechnol 23(6):1815–1820.  https://doi.org/10.1007/s10068-014-0248-4 CrossRefGoogle Scholar
  9. Danphitsanuparn P, Boonsnongcheep P, Boriboonkaset T, Chintapakorn Y, Prathanturarug S (2012) Effects of Agrobacterium rhizogenes strains and other parameters on production of isoflavonoids in hairy roots of Pueraria candollei Grah. ex Benth. var. candollei. Plant Cell Tissue Org 111(3):315–322.  https://doi.org/10.1007/s11240-012-0196-8 CrossRefGoogle Scholar
  10. Jackson RL, Greiwe JS, Schwen RJ (2011) Emerging evidence of the health benefits of S-equol, an estrogen receptor beta agonist. Nutr Rev 69(8):432–448.  https://doi.org/10.1111/j.1753-4887.2011.00400.x CrossRefPubMedGoogle Scholar
  11. Jeong YJ, An CH, Park SC, Pyun JW, Lee J, Kim SW, Kim HS, Kim H, Jeong JC, Kim CY (2018) Methyl jasmonate increases isoflavone production in soybean cell cultures by activating structural genes involved in isoflavonoid biosynthesis. J Agric Food Chem 66(16):4099–4105.  https://doi.org/10.1021/acs.jafc.8b00350 CrossRefPubMedGoogle Scholar
  12. Lamlertkittikul S, Chandeying V (2004) Efficacy and safety of Pueraria mirifica (Kwao Kruea Khao) for the treatment of vasomotor symptoms in perimenopausal women: Phase II Study. J Med Assoc Thail 87(1):33–40Google Scholar
  13. Lephart ED (2016) Skin aging and oxidative stress: Equol’s anti-aging effects via biochemical and molecular mechanisms. Ageing Res Rev 31:36–54.  https://doi.org/10.1016/j.arr.2016.08.001 CrossRefPubMedGoogle Scholar
  14. Matsumura A, Ghosh A, Pope GS, Darbre PD (2005) Comparative study of oestrogenic properties of eight phytoestrogens in MCF7 human breast cancer cells. J Steroid Biochem Mol Biol 94(5):431–443.  https://doi.org/10.1016/j.jsbmb.2004.12.041 CrossRefPubMedGoogle Scholar
  15. Satdive RK, Fulzele DP, Eapen S (2007) Enhanced production of azadirachtin by hairy root cultures of Azadirachta indica A. Juss by elicitation and media optimization. J Biotechnol 128(2):281–289.  https://doi.org/10.1016/j.jbiotec.2006.10.009 CrossRefPubMedGoogle Scholar
  16. Sugiyama H, Kumamoto T, Suganami A, Nakanishi W, Sowa Y, Takiguchi M, Ishikawa T, Tamura Y (2009) Insight into estrogenicity of phytoestrogens using in silico simulation. Biochem Bioph Res Commun 379(1):139–144.  https://doi.org/10.1016/j.bbrc.2008.12.046 CrossRefGoogle Scholar
  17. Suzuki H, Reddy MS, Naoumkina M, Aziz N, May GD, Huhman DV, Sumner LW, Blount JW, Mendes P, Dixon RA (2005) Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula. Planta 220(5):696–707.  https://doi.org/10.1007/s00425-004-1387-2 CrossRefPubMedGoogle Scholar
  18. Tebayashi S, Ishihara A, Iwamura H (2001) Elicitor-induced changes in isoflavonoid metabolism in red clover roots. J Exp Bot 52(357):681–689CrossRefGoogle Scholar
  19. Udomsuk L, Jarukamjorn K, Tanaka H, Putalun W (2009) Isoflavonoid production in a hairy roots culture of Pueraria candollei. Z Naturforsch C 64(9–10):687–691CrossRefGoogle Scholar
  20. Udomsuk L, Jarukamjorn K, Tanaka H, Putalun W (2011) Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation. Biotechnol Lett 33(2):369–374.  https://doi.org/10.1007/s10529-010-0417-3 CrossRefPubMedGoogle Scholar
  21. Udomsuk L, Juengwatanatrakul T, Putalun W, Jarukamjorn K (2012a) Bimodal action of miroestrol and deoxymiroestrol, phytoestrogens from Pueraria candollei var. mirifica, on hepatic CYP2B9 and CYP1A2 expressions and antilipid peroxidation in mice. Nutr Res 32(1):45–51.  https://doi.org/10.1016/j.nutres.2011.11.003 CrossRefPubMedGoogle Scholar
  22. Udomsuk L, Juengwattanatrakul T, Jarukamjorn K, Putalun W (2012b) Increased miroestrol, deoxymiroestrol and isoflavonoid accumulation in callus and cell suspension cultures of Pueraria candollei var. mirifica. Acta Physiol Plant 34(3):1093–1100.  https://doi.org/10.1007/s11738-011-0906-6 CrossRefGoogle Scholar
  23. Utian WH, Jones M, Setchell KD (2015) S-equol: a potential nonhormonal agent for menopause-related symptom relief. J Womens Health (Larchmt) 24(3):200–208.  https://doi.org/10.1089/jwh.2014.5006 CrossRefGoogle Scholar
  24. Vasconsuelo A, Boland R (2007) Molecular aspects of the early stages of elicitation of secondary metabolites in plants. Plant Sci 172(5):861–875.  https://doi.org/10.1016/j.plantsci.2007.01.006 CrossRefGoogle Scholar
  25. Yusakul G, Putalun W, Udomsin O, Juengwatanatrakul T, Chaichantipyuth C (2011) Comparative analysis of the chemical constituents of two varieties of Pueraria candollei. Fitoterapia 82(2):203–207.  https://doi.org/10.1016/j.fitote.2010.09.009 CrossRefPubMedGoogle Scholar
  26. Yusakul G, Udomsin O, Juengwatanatrakul T, Tanaka H, Chaichantipyuth C, Putalun W (2013a) High performance enzyme-linked immunosorbent assay for determination of miroestrol, a potent phytoestrogen from Pueraria candollei. Anal Chim Acta 785(Supplement C):104–110.  https://doi.org/10.1016/j.aca.2013.04.053 CrossRefPubMedGoogle Scholar
  27. Yusakul G, Udomsin O, Juengwatanatrakul T, Tanaka H, Chaichantipyuth C, Putalun W (2013b) Highly selective and sensitive determination of deoxymiroestrol using a polyclonal antibody-based enzyme-linked immunosorbent assay. Talanta 114(Supplement C):73–78.  https://doi.org/10.1016/j.talanta.2013.04.011 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Orapin Udomsin
    • 1
    • 2
  • Gorawit Yusakul
    • 3
    • 4
  • Witsarut Kraithong
    • 1
    • 2
  • Latiporn Udomsuk
    • 5
  • Tharita Kitisripanya
    • 6
  • Thaweesak Juengwatanatrakul
    • 7
  • Waraporn Putalun
    • 1
    • 2
  1. 1.Faculty of Pharmaceutical SciencesKhon Kaen UniversityKhon KaenThailand
  2. 2.Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB)National Research University-Khon Kaen UniversityKhon KaenThailand
  3. 3.Drug and Cosmetics Excellence CenterWalailak UniversityNakhon Si ThammaratThailand
  4. 4.School of PharmacyWalailak UniversityNakhon Si ThammaratThailand
  5. 5.College of Medicine and Public HealthUbon Ratchatani UniverstyUbon RatchataniThailand
  6. 6.Department of Pharmacognosy, Faculty of PharmacyMahidol UniversityBangkokThailand
  7. 7.Faculty of Pharmaceutical SciencesUbon Ratchathani UniversityUbon RatchathaniThailand

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