The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr.
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Plant cell and tissue cultures are considered as a source of valuable secondary metabolites but usually produce insufficient level of the compounds, which is the limiting factor for their application in biotechnology. We obtained 18 callus cell cultures from different organs of wild grape Vitis amurensis Rupr. collected at different seasons and analyzed stilbene accumulation in combination with calli growth parameters. This analysis showed that temporal and tissue origin of the calli affected the rate of stilbene biosynthesis. Stem-derived calli accumulated higher stilbene levels and exhibited a higher expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes than calli derived from the leaves and petioles. The highest content of stilbenes was detected in the calli initiated from grapevine stems collected in the autumn. In general, all “autumn” cell cultures contained more than 2 mg g− 1 dry wt (up to 11 mg g− 1 dry wt) and exhibited high PAL and STS genes expression in comparison with the calli initiated in the summer. The content of stilbenes in the “autumn” cell cultures were comparable to the highest stilbene contents detected in other plant sources described in the literature. Thus, selecting the most optimal explant source for cell culture establishment could be an effective approach towards developing plant cell cultures producing high stilbene levels.
KeywordsPiceid Resveratrol Stilbenoid glucoside Stilbene synthase STS Viniferin
High-performance liquid chromatography
This work was supported by a Grant from the Russian Science Foundation (17-74-10082).
ASD and KKV were involved in research design, data analysis, and paper preparation. APT, ARS, and NNN were involved in plant cell cultures management and qRT-PCR. MAY performed HPLC. YAK: analyzed the data.
Compliance with ethical standards
Conflict of interest
We declare that we have no conflict of interest.
- Giorcelli A, Sparvoli F, Mattivi F, Tava A, Balestrazzi A, Vrhovsek U, Calligari P, Bollini R, Confalonieri M (2004) Expression of the stilbene synthase (StSy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant resveratrol glucosides. Transgenic Res 13:203–214CrossRefGoogle Scholar
- Keskin N, Kunter B (2008) Production of trans-resveratrol in ‘‘Cabernet Sauvignon’’ (Vitis vinifera L.) callus culture in response to ultraviolet-C irradiation. Vitis 47:193–196Google Scholar
- Kiselev KV, Dubrovina AS, Shumakova OA, Karetin YA, Manyakhin AY (2013) Structure and expression profiling of a novel calcium-dependent protein kinase gene, CDPK3a, in leaves, stems, grapes, and cell cultures of wild-growing grapevine Vitis amurensis Rupr.. Plant Cell Rep 32:431–442CrossRefGoogle Scholar
- Margara J, Piollat MT (1983) New observations on in vitro organogenesis from begonia x elator petals. Comptes Rendus de l’Académie des Sciences - Series III - Sciences de la Vie 297: 161–164Google Scholar
- Martini AN, Papafotiou M, Vemmos SN (2013) Season and explant origin affect phenolic content, browning of explants, and micropropagation of X Malosorbus florentina (Zucc.) Browicz. Hortscience 48:102–107Google Scholar
- Santamaria AR, Innocenti M, Mulinacci N, Melani F, Valletta A, Sciandra I, Pasqua G (2012) Enhancement of viniferin production in Vitis vinifera L. cv. Alphonse Lavallée cell suspensions by low-energy ultrasound alone and in combination with methyl jasmonate. J Agric Food Chem 60:11135–11142CrossRefGoogle Scholar
- Schwekendiek A, Spring O, Heyerick A, Pickel B, Pitsch NT, Peschke F, Keukeleire DD, Weber G (2007) Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. J Agric Food Chem 55:7002–7009CrossRefGoogle Scholar
- Shankar S, Nall D, Tang SN, Meeker D, Passarini J, Sharma J, Srivastava RK (2011) Resveratrol inhibits pancreatic cancer stem cell characteristics in human and kras(G12D) transgenic mice by inhibiting pluripotency maintaining factors and epithelial-mesenchymal transition. PLoS ONE 6:e16530CrossRefGoogle Scholar