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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 136, Issue 1, pp 189–196 | Cite as

The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr.

  • A. P. Tyunin
  • A. R. Suprun
  • N. N. Nityagovsky
  • A. Y. Manyakhin
  • Y. A. Karetin
  • A. S. Dubrovina
  • K. V. KiselevEmail author
Research Note
  • 168 Downloads

Abstract

Plant cell and tissue cultures are considered as a source of valuable secondary metabolites but usually produce insufficient level of the compounds, which is the limiting factor for their application in biotechnology. We obtained 18 callus cell cultures from different organs of wild grape Vitis amurensis Rupr. collected at different seasons and analyzed stilbene accumulation in combination with calli growth parameters. This analysis showed that temporal and tissue origin of the calli affected the rate of stilbene biosynthesis. Stem-derived calli accumulated higher stilbene levels and exhibited a higher expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes than calli derived from the leaves and petioles. The highest content of stilbenes was detected in the calli initiated from grapevine stems collected in the autumn. In general, all “autumn” cell cultures contained more than 2 mg g− 1 dry wt (up to 11 mg g− 1 dry wt) and exhibited high PAL and STS genes expression in comparison with the calli initiated in the summer. The content of stilbenes in the “autumn” cell cultures were comparable to the highest stilbene contents detected in other plant sources described in the literature. Thus, selecting the most optimal explant source for cell culture establishment could be an effective approach towards developing plant cell cultures producing high stilbene levels.

Keywords

Piceid Resveratrol Stilbenoid glucoside Stilbene synthase STS Viniferin 

Abbreviations

HPLC

High-performance liquid chromatography

STS

Stilbene synthase

Wt

Weight

Notes

Acknowledgements

This work was supported by a Grant from the Russian Science Foundation (17-74-10082).

Author Contributions

ASD and KKV were involved in research design, data analysis, and paper preparation. APT, ARS, and NNN were involved in plant cell cultures management and qRT-PCR. MAY performed HPLC. YAK: analyzed the data.

Compliance with ethical standards

Conflict of interest

We declare that we have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • A. P. Tyunin
    • 1
  • A. R. Suprun
    • 1
    • 2
  • N. N. Nityagovsky
    • 1
    • 2
  • A. Y. Manyakhin
    • 1
  • Y. A. Karetin
    • 2
    • 3
  • A. S. Dubrovina
    • 1
  • K. V. Kiselev
    • 1
    • 2
    Email author
  1. 1.Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial BiodiversityFar Eastern Branch of the Russian Academy of SciencesVladivostokRussia
  2. 2.Department of Biodiversity, The School of Natural SciencesFar Eastern Federal UniversityVladivostokRussia
  3. 3.Laboratory of Embryology, National Scientific Center of Marine BiologyFar Eastern Branch of the Russian Academy of SciencesVladivostokRussia

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