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Toxicity impacts of chemically and biologically synthesized CuO nanoparticles on cell suspension cultures of Nicotiana tabacum

  • Sepideh Mahjouri
  • Ali Movafeghi
  • Baharak Divband
  • Morteza Kosari-Nasab
Original Article
  • 10 Downloads

Abstract

Nanotechnology has quite a lot of applications in various fields of industrial sectors like food and agriculture. Although nanotechnology can improve the quality of life, its possible associated risks should be assessed. Here copper oxide nanoparticles (CuO NPs) were synthesized by chemical (polymer pyrolysis) and biological (green) methods with an average size of 30 and 44 nm, respectively. Afterwards, a cell biology approach was applied to evaluate the toxic effects of chemically and biologically synthesized CuO nanoparticles on tobacco cell suspension cultures. Both types of CuO nanoparticles significantly dropped the viability of the cells in a dose and time dependent manner. Accordingly, tobacco cells were found to increase the activity of antioxidant enzymes after 48 h of exposure to nanoparticles. The production of reactive oxygen species (ROS) and malondialdehyde (MDA) in a dose dependent manner was also observed. Assessment of the toxicity of CuO NPs revealed that chemically synthesized NPs were more toxic than biologically synthesized ones. It can be concluded that the organic components of the plant extract as capping agents that remain on the surface of green synthesized CuO NPs may reduce their toxicity effects.

Keywords

Nicotiana tabacum Cell culture Cytotoxicity CuO nanoparticles Green synthesis 

Abbreviations

CS-CuO NPs

Chemically synthesized copper oxide nanoparticles

GS-CuO NPs

Green synthesized copper oxide nanoparticles

MDA

Malondialdehyde

ROS

Reactive Oxygen Species

SOD

Superoxide dismutase

POD

Peroxidase

CAT

Catalase

Notes

Acknowledgements

The authors thank the University of Tabriz (Iran) and the Hayyan Biotechnology Laboratory for scientific and financial supports.

Author contributions

AM and SM was responsible for the design and overall investigation. BD helped in synthesis and analysis of nanoparticles. SM and MK-N conducted the cell culture experiments. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Plant Biology, Faculty of Natural SciencesUniversity of TabrizTabrizIran
  2. 2.Department of Inorganic Chemistry, Faculty of ChemistryTabriz UniversityTabrizIran
  3. 3.Drug Applied Research CenterTabriz University of Medical SciencesTabrizIran

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