Toxicity impacts of chemically and biologically synthesized CuO nanoparticles on cell suspension cultures of Nicotiana tabacum
- 55 Downloads
Nanotechnology has quite a lot of applications in various fields of industrial sectors like food and agriculture. Although nanotechnology can improve the quality of life, its possible associated risks should be assessed. Here copper oxide nanoparticles (CuO NPs) were synthesized by chemical (polymer pyrolysis) and biological (green) methods with an average size of 30 and 44 nm, respectively. Afterwards, a cell biology approach was applied to evaluate the toxic effects of chemically and biologically synthesized CuO nanoparticles on tobacco cell suspension cultures. Both types of CuO nanoparticles significantly dropped the viability of the cells in a dose and time dependent manner. Accordingly, tobacco cells were found to increase the activity of antioxidant enzymes after 48 h of exposure to nanoparticles. The production of reactive oxygen species (ROS) and malondialdehyde (MDA) in a dose dependent manner was also observed. Assessment of the toxicity of CuO NPs revealed that chemically synthesized NPs were more toxic than biologically synthesized ones. It can be concluded that the organic components of the plant extract as capping agents that remain on the surface of green synthesized CuO NPs may reduce their toxicity effects.
KeywordsNicotiana tabacum Cell culture Cytotoxicity CuO nanoparticles Green synthesis
- CS-CuO NPs
Chemically synthesized copper oxide nanoparticles
- GS-CuO NPs
Green synthesized copper oxide nanoparticles
Reactive Oxygen Species
The authors thank the University of Tabriz (Iran) and the Hayyan Biotechnology Laboratory for scientific and financial supports.
AM and SM was responsible for the design and overall investigation. BD helped in synthesis and analysis of nanoparticles. SM and MK-N conducted the cell culture experiments. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Acharyulu N, Dubey R, Swaminadham V, Kollu P, Kalyani R, Pammi S (2014) Green synthesis of CuO nanoparticles using Phyllanthus amarus leaf extract and their antibacterial activity against multidrug resistance bacteria. Int J Engin Res Technol 3:639–641Google Scholar
- Adhikari T, Kundu S, Biswas AK, Tarafdar JC, Rao AS (2012) Effect of copper oxide nanoparticle on seed germination of selected crops. J Agric Sci Technol 2:815–823Google Scholar
- Hong J, Rico CM, Zhao L, Adeleye AS, Keller AA, Peralta-Videa JR, Gardea-Torresdey JL (2015) Toxic effects of copper-based nanoparticles or compounds to lettuce (Lactuca sativa) and alfalfa (Medicago sativa). Environ Sci Proc Impacts 17:177–185. https://doi.org/10.1039/C4EM00551A CrossRefGoogle Scholar
- Javed R, Mohamed A, Yücesan B, Gürel E, Kausar R, Zia M (2017) CuO nanoparticles significantly influence in vitro culture, steviol glycosides, and antioxidant activities of Stevia rebaudiana Bertoni. Plant Cell Tiss Organ Cult 131:611–620. https://doi.org/10.1007/s11240-017-1312-6 CrossRefGoogle Scholar
- Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud 15:523–530Google Scholar
- Nasrollahzadeh M, Maham M, Sajadi SM (2015) Green synthesis of CuO nanoparticles by aqueous extract of Gundelia tournefortii and evaluation of their catalytic activity for the synthesis of N-monosubstituted ureas and reduction of 4-nitrophenol. J Colloid Interface Sci 455:245–253. https://doi.org/10.1016/j.jcis.2015.05.045 CrossRefPubMedGoogle Scholar
- Pietrowska-Borek M, Chadzinikolau T, Kozlowska M (2010) Effect of urban pollution on 4-coumarate: CoA ligase and flavonoid accumulation in Berberis thunbergii. Dendrobiology 64:79–85Google Scholar
- Rai V, Vajpayee P, Singh SN, Mehrotra S (2004) Effect of chromium accumulation on photosynthetic pigments, oxidative stress defense system, nitrate reduction, proline level and eugenol content of Ocimum tenuiflorum L. Plant Sci 167:1159–1169. https://doi.org/10.1016/j.plantsci.2004.06.016 CrossRefGoogle Scholar
- Rico C, Peralta-Videa J, Gardea-Torresdey J (2015) Chemistry, biochemistry of nanoparticles, and their role in antioxidant defense system in plants. In: Siddiqui MH, Al-Whaib M (eds) Nanotechnology and plant sciences. Springer, Cham, pp 1–17Google Scholar
- Song U, Jun H, Waldman B, Roh J, Kim Y, Yi J, Lee EJ (2013) Functional analyses of nanoparticle toxicity: a comparative study of the effects of TiO2 and Ag on tomatoes (Lycopersicon esculentum). Ecotoxicol Environ Saf 93:60–67. https://doi.org/10.1016/j.ecoenv.2013.03.033 CrossRefPubMedGoogle Scholar