Early expression of WUSCHEL is a marker for in vitro shoot morphogenesis in tobacco and Beta palonga
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We have studied the role of growth regulators behind in vitro shoot organogenesis and somatic embryogenesis in two plant systems, viz. tobacco (Nicotiana tabacum L. var. Jayasri) and Beta palonga R.K. Basu & K.K. Mukh. We have also correlated the phenomena of de differentiation with the relative expression of WUS (WUSCHEL) gene in a time-dependent manner. The results indicated that early WUS gene expression is a definite marker for in vitro shoot organogenesis in tobacco and Beta both in direct and indirect modes of regeneration. Additionally, we have performed a comparative homology modeling and in silico structural analysis of WUSCHEL proteins of B. palonga, B. vulgaris, and Arabidopsis to find out the commonality of the ligand binding site. The amino acids of the binding sites were identical (Arginine, Tryptophan, Proline, Asparagine, and Tyrosine) in the three materials under study; except two additional amino acids (Isoleucine and Alanine) in B. vulgaris.
KeywordsIn vitro morphogenesis Beta Tobacco WUSCHEL Homology modeling Docking qRT-PCR
We acknowledge the guidance and encouragement of Professor K. K. Mukherjee, Bose Institute. Authors are grateful to the Director of Bose Institute for providing financial and infrastructural support. The financial assistance in form of research fellowship provided by UGC (University Grants Commission), India is acknowledged by the first author (MS). Technical assistance of Mr. Jadab Ghosh and Mrs. Kaberi Ghosh is also duly acknowledged.
MS, GG: conceived and designed the experiments. GG: performed the tissue culture experiments. MS: performed the molecular biology experiments. GG: wrote the paper.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The manuscript complies with the Ethical Rules applicable for Plant Cell Tissue and Organ Culture.
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