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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 134, Issue 2, pp 251–265 | Cite as

Quantitative profiling of polyacetylenes in tissue cultures and plant parts of three species of the Asteraceae

  • Nargis F. A. Elgahme
  • Ute Wittstock
Original Article
  • 92 Downloads

Abstract

Polyacetylenes are a group of fatty-acid derived specialized metabolites with several C–C-triple bonds and derived compounds which are widely distributed in the plant kingdom, but are especially abundant and structurally diverse in the Asteraceae family. Despite their interesting structural and biological properties, the biosynthesis of polyacetylenes is only poorly understood. We have used three species of the Asteraceae (Carthamus tinctorius, Tagetes patula, and Arctium lappa) to compare their suitability for studies of polyacetylene biosynthesis when used after cultivation on soil or as tissue culture. The polyacetylene profiles detected in different plant parts together with information from the literature indicate that C. tinctorius seedlings and flowers as well as T. patula roots and flower buds are major sites of polyacetylene biosynthesis. Highest levels of polyacetylenes were detected in T. patula [about 30 µmol/g dry weight (d.w.) thiophenes in roots] while A. lappa contained less than 1 µmol/g d.w.. Methyljasmonate (MeJ)-induced T. patula hairy root cultures proved to be an excellent source of butenynyl-bithiophene (200 µmol/g d.w., 43 mg/g d.w.) while T. patula flower buds could serve as a source of pentenynyl-bithiophene and α-terthienyl (5–10 µmol/g d.w.) and C. tinctorius flowers or seedlings as a source of polyacetylenic C13 hydrocarbons, the biosynthetic precursors of thiophenes (5–10 µmol/g d.w.). Upon addition of elicitors to tissue cultures, highest elicitation factors (between four and seven) were reached for 1,11-tridecadiene-3,5,7,9-tetrayne in C. tinctorius cell suspension cultures with 40 µM MeJ and α-terthienyl in T. patula hairy root cultures with 100 µM MeJ.

Keywords

Carthamus tinctorius Tagetes patula Arctium lappa Thiophenes α-Terthienyl 1,11-Tridecadiene-3,5,7,9-tetrayne 

Notes

Acknowledgements

Dr. Till Beuerle is thanked for advice on compound identification and quantification by GC. Financial support of N.F.A.E. by a PhD Fellowship of the Libyan Government is gratefully acknowledged.

Author contributions

N.F.A.E. designed and performed experiments and analyzed data. U.W. conceived the study with contributions from N.F.A.E., discussed results, and wrote the manuscript based on a draft prepared by N.F.A.E.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11240_2018_1417_MOESM1_ESM.pdf (389 kb)
Supplementary material 1 (PDF 389 KB)

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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Pharmaceutical BiologyTechnische Universität BraunschweigBraunschweigGermany

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