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In vitro multiplication of wild relatives in genus Beta conserves the invaluable threatened germplasms


Conservation of threatened plant genetic resources is vital for food security and biodiversity maintenance. Wild species of genus Beta are invaluable germplasm which could be utilized for beet breeding programs. In this study, in vitro multiplication and genotypic responses of different species in the section Beta and Corollinae were compared in presence of plant growth regulators. Following the surface sterilization, seeds were cultivated in vitro. Cotyledonary node explants were excised from the germinated young seedlings and treated with BAP, GA3 and TDZ. The obtained results demonstrated a significant difference between the genotypes in terms of in vitro shoot proliferation efficiency when subjected to BAP and GA3. Among the applied growth regulators, effect of BAP on number of multiplied shoots was the highest. Moreover, the genotypic variation in rooting was also evident when the shoots were subjected to IAA and IBA. This study is the first report of in vitro multiplication of wild species in genus Beta that could be exploited in future germplasm maintenance, gene transformation and breeding studies.

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Gibberellic acid


Indole-3-acetic acid


Indole-3-butyric acid


Murashige and Skoog Basal Medium




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The financial support from the Scientific and Technological Research Council of Turkey (TUBITAK), Project No: TOVAG-113O096, is deeply appreciated. Also, the authors are thankful to the Aegean Agricultural Research Institute, Izmir, Turkey, for kindly providing the seeds required for the study.

Author information

AE designed and outlined the research. SDK contributed to data analysis, interpreted the data and wrote the manuscript. MÇO analyzed the data. MÇO and CYÖ performed the experiment. SG and EG criticized the data interpretation and edited the manuscript.

Correspondence to A. Ergül.

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The authors declare that they have no conflict of interest.

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Communicated by Sergio J. Ochatt.

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Ergül, A., Khabbazi, S.D., Oğuz, M.Ç. et al. In vitro multiplication of wild relatives in genus Beta conserves the invaluable threatened germplasms. Plant Cell Tiss Organ Cult 134, 169–175 (2018).

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  • Wild beet
  • In vitro multiplication
  • Tissue culture
  • Germplasm conservation