Functional analysis of different promoter haplotypes of the coffee (Coffea canephora) CcDREB1D gene through genetic transformation of Nicotiana tabacum

  • Sinara Oliveira de Aquino
  • Fernanda de Araújo Carneiro
  • Erica Cristina Silva Rêgo
  • Gabriel Sergio Costa Alves
  • Alan Carvalho Andrade
  • Pierre Marraccini
Original Article
  • 44 Downloads

Abstract

Previous results showed that the three promoter haplotypes (HP15, HP16 and HP17) of the CcDREB1D gene (encoding the dehydration responsive element binding transcription factor) found in the drought-tolerant (HP15/HP16) and drought-sensitive (HP15/HP17) clones of Coffea canephora, diverged by several single nucleotide polymorphisms and insertions/deletions. In order to compare the activities and regulation of these haplotypes in response to abiotic stresses, these sequences were cloned in front of the uidA and analyzed in transgenic tobacco (Nicotiana tabacum) for their ability to regulate the expression of this reporter gene by monitoring GUS histochemical activity under drought (mimicked by dehydration), heat shock and cold treatments. Under unstressed condition, GUS staining was mainly observed in leaf and root vascular tissues of young tobacco plants transformed by the longest sequences of CcDREB1D promoter haplotypes. These GUS activities were not observed in the same tissues of older plants as well as in plants transformed by shorter proximal regions, suggesting a developmentally-regulated activity of CcDREB1D promoters in tobacco and the existence of cis-regulatory elements essential for their regulation in distal regions. Under dehydration and heat shock conditions, GUS staining detected in leaf midribs and secondary veins of pHP17L-transformed plants was correlated with up-regulated expression of uidA reporter gene while no GUS activities were observed in pHP16L-transformed plants. However, all CcDREB1D promoter haplotypes were positively regulated by cold stress in transgenic tobacco. These results showed that these coffee promoters were recognized by the tobacco transcriptional machinery but were regulated in different manners in response to abiotic stress.

Keywords

Abiotic stress Coffee DREB1D Promoter haplotypes Tobacco 

Abbreviations

ABA

Abscisic acid

DREB

Dehydration responsive element binding transcription factor

GUS

ß-glucuronidase

Supplementary material

11240_2017_1328_MOESM1_ESM.tif (66 kb)
Supplementary Figure 1— Schematic representation of CcDREB1D promoter constructions. (TIF 66 KB)

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© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Sinara Oliveira de Aquino
    • 1
    • 2
  • Fernanda de Araújo Carneiro
    • 1
  • Erica Cristina Silva Rêgo
    • 2
  • Gabriel Sergio Costa Alves
    • 1
    • 2
  • Alan Carvalho Andrade
    • 2
  • Pierre Marraccini
    • 2
    • 3
    • 4
  1. 1.Federal University of Lavras UFLALavrasBrazil
  2. 2.EMBRAPA Genetic Resources and BiotechnologyBrasiliaBrazil
  3. 3.CIRAD, UMR AGAPMontpellierFrance
  4. 4.CIRAD, UMR IPME (University Montpellier, CIRAD, IRD, Montpellier), Agricultural Genetics Institute, LMI RICE2HanoiVietnam

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