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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 132, Issue 1, pp 191–199 | Cite as

Analysis of the promoter features of BpCUC2 in Betula platyphylla × Betula pendula

  • Chaoyi Liu
  • Huanwen Xu
  • Jing Jiang
  • Sui Wang
  • Guifeng LiuEmail author
Original Article

Abstract

CUC2 encodes a NAC domain transcription factor required for establishing boundaries around plant organ primordia. It is also associated with the initiation of the shoot apical meristem. We cloned a 1497-bp BpCUC2 promoter fragment and constructed a pBpCUC2::LUC vector for birch transgenic. A quantitative real-time polymerase chain reaction (qRT-PCR) analysis of transgenic birch buds, leaves, stems, and roots revealed that luciferase (LUC) was most highly expressed in the buds. The pBpCUC2::LUC transgenic birch plants were treated with methyl jasmonate, indoleacetic acid, abscisic acid, or gibberellic acid3 for 2 h. The subsequent qRT-PCR analysis indicated that the transgenic birch tissues responded differently to the various hormones. Our results confirmed that BpCUC2 expression is influenced by hormones. The PLACE online tool revealed that the BpCUC2 promoter sequence contains several cis-acting elements. Furthermore, an auxin response element was used to screen transcription factors in a yeast one-hybrid assay. We identified three unique cDNA sequences, with complete open reading frames containing regulatory motifs, which were related to growth and development.

Keywords

Betula platyphylla × Betula pendula Promoter expression feature Gene function Upstream regulatory gene screening 

Abbreviations

CUC2

CUP-SHAPED COTYLEDON2

SAM

Shoot apical meristem

BpCUC2

Betula platyphylla × Betula pendula CUC2

pBpCUC2

BpCUC2 promoter

LUC

Luciferase

PINs

PIN-formed proteins

SD

Synthetic dropout medium

Leu

Leudne

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China “Cloning and Functional Study on the Leaf Margin-related Genes of Betula platyphylla” Grant (Grant No. 31370660) and 111 Project (B16010). The funding source had no other involvement.

Authors’ contribution

CL, HX: performed all the experiments. SW assisted with DNA sequence analysis. GL, JJ together with CL, HX and SW designed the experiments and wrote the manuscript. All authors read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11240_2017_1324_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 KB)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinChina

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