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Epigenetics of long-term somatic embryogenesis in Theobroma cacao L.: DNA methylation and recovery of embryogenic potential

Abstract

In Theobroma cacao L., declined embryogenic potential was observed in regenerated somatic embryos from long-term secondary somatic embryogenesis (SE). In order to explore the relationship between DNA methylation and the long-term secondary SE, the embryogenic potential and global DNA methylation levels in young (12 months-old), aged (36 months-old) and extra somatic embryogenesis (39 months-old) subjected to different 5-Azacytidine (5-azaC) treatments were comparatively assessed. Global DNA methylation levels increased in aged somatic embryos with long-term in vitro culture, but 5-azaC-supplemented treatments resulted in unaltered levels. In addition, DNA methylation pattern during SE was not affected by 5-azaC. DNA methylation increased during SE expression. Interestingly, the extra SE induction showed that aged somatic embryos can recovery the embryogenic potential in treatment supplemented with 5-azaC at specific concentration. The outcome of this study suggested that the long-term SE in cacao induced the decline on embryogenic potential, which can be reversible trough 5-azaC supplementation. Besides, increased DNA methylation levels might be a response to the stress conditions that plant cells were exposed to during SE.

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Acknowledgements

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Proc. 478393/2013-0, and 306126/2013-3), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Fundação de Amparo a Pesquisa e Inovação do Estado de Santa Catarina (FAPESC, Proc. 2780/2012-4). The authors thank to Central Laboratory of Electron Microscopy (LCME) of the Federal University of Santa Catarina, Brazil.

Author information

LAPQ and MPG: Conceived, designed and conducted the experiments. LAPQ and MPG: Data analysis and manuscript writing. HPFF and LNV: DNA methylation analysis and manuscript review. MPG: Contributed reagents/materials/analysis tools.

Correspondence to Miguel Pedro Guerra.

Additional information

Communicated by M. Angeles Revilla.

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Pila Quinga, L.A., Pacheco de Freitas Fraga, H., do Nascimento Vieira, L. et al. Epigenetics of long-term somatic embryogenesis in Theobroma cacao L.: DNA methylation and recovery of embryogenic potential. Plant Cell Tiss Organ Cult 131, 295–305 (2017). https://doi.org/10.1007/s11240-017-1284-6

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Keywords

  • Somatic embryo development
  • 5-Azacytidine
  • DNA methylation inhibitor
  • Subcultures
  • In vitro morphogenesis