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Elicitor enhanced production of protoberberine alkaloids from in vitro cell suspension cultures of Tinospora cordifolia (Willd.) Miers ex Hook. F. & Thoms

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Abstract

The present research investigates the effect of Piriformospora indica, an endophytic fungus, on production of protoberberine alkaloids in in vitro cell suspension cultures of Tinospora cordifolia. Although T. cordifolia produces a number of protoberberine alkaloids, the simultaneous production of jatrorrhizine and palmatine in cell suspension cultures of T. cordifolia was observed for the first time with the use of P. indica as biotic elicitor. The cells in suspension cultures were elicitated with P. indica on 14th day of culture initiation and the production of the alkaloids on 16th day was monitored. The autoclaved as well as filter sterilized cultures of P. indica were used in addition to the use of fungal cell extract. The elicitor effect of P. indica was analyzed and compared with other abiotic elicitor (methyl jasmonate) and biotic elicitors (chitin and chitosan). The culture filtrate of P. indica in the filter sterilized (5.0% v/v) form gave better response with enhanced 4.2-fold production of jatrorrhizine (10.72 mg/g DW) and 4.0-fold production of palmatine (4.39 mg/g DW). The production of these compounds was at par with that achieved in methyl jasmonate (at 250 µM) treated cell suspension cultures.

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Acknowledgements

The authors are thankful to Mr Sumit Singh, Department of Chemical Engineering, Indian Institute of Technology Delhi, New Delhi for assisting in MS data analysis and to Department of Biotechnology, Ministry of Science and Technology, Govt. of India for partially supporting the work.

Author information

Correspondence to V. S. Bisaria.

Additional information

Communicated by Wagner Campos Otoni.

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Kumar, P., Srivastava, V., Chaturvedi, R. et al. Elicitor enhanced production of protoberberine alkaloids from in vitro cell suspension cultures of Tinospora cordifolia (Willd.) Miers ex Hook. F. & Thoms. Plant Cell Tiss Organ Cult 130, 417–426 (2017). https://doi.org/10.1007/s11240-017-1237-0

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Keywords

  • Abiotic elicitor
  • Biotic elicitor
  • Jatrorrhizine
  • Palmatine
  • Piriformospora indica