Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 130, Issue 2, pp 255–263 | Cite as

A comparative study on growth and morphology of wasabi plantlets under the influence of the micro-environment in shoot and root zones during photoautotrophic and photomixotrophic micropropagation

Original Article
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Abstract

The growth of wasabi (Wasabia japonica Matsumura) plantlets under different micro-environments inside culture vessels in photoautotrophic micropropagation (PA) and photomixotrophic micropropagation (PM) conditions were compared. After 28 days of culture, dry weight, relative growth rate, leaf area, and leaf chlorophyll contents of plantlets in PA were greater than those in PM. The number of leaves did not differ significantly between PA and PM conditions. PA promoted root growth and development with a greater number of roots, root length, root diameter, root fresh weight, root dry weight, and root xylem vessel system. Dissolved oxygen concentration in PA culture medium sharply decreased after 7 days of culture and then recovered. In PM culture medium, no significant fluctuation of dissolved oxygen concentration was apparent. The net photosynthetic rates of plantlets in PA were much higher than those in PM and increased with culture time. In contrast, the net photosynthetic rates of wasabi plantlets in PM kept a low and constant value during the culture period. With the presence of gas exchange membranes attached to the vessel lids, the detected vapor pressure deficit was higher in PA than in PM conditions. Higher stomatal density and larger stomatal aperture on the abaxial and adaxial surfaces of the leaves in PM medium promoted leaf water loss following ex vitro conditions. Thus, PA is applicable for producing healthy wasabi transplants.

Keywords

Dissolved oxygen concentration Evapotranspiration Medium water loss Photosynthesis Wasabia japonica 

Abbreviations

PA

Photoautotrophic micropropagation

PM

Photomixotrophic micropropagation

FW

Fresh weight

DW

Dry weight

RGR

Relative growth rate

S/R

Shoot/root dry weight ratio

WC

Water content

WLR

Water loss rate

Pn

Net photosynthetic rate

RH

Relative humidity

VPD

Vapor pressure deficit

DO

Dissolved oxygen

References

  1. Chadwick CI, Lumpkin TA, Elberson LR (1993) The botany, uses and production of Wasabia japonica (Miq.) (Cruciferae) Matsum. Economic Bot 47:113–135. doi:10.1007/BF02862015 CrossRefGoogle Scholar
  2. Couceiro MA, Afreen F, Zobayed SMA, Kozai T (2006) Enhanced growth and quality of St. John’s wort (Hypericum perforatum L.) under photoautotrophic in vitro conditions. In vitro Cell Dev Biol Plant 42:278–282. doi:10.1079/IVP2006752 CrossRefGoogle Scholar
  3. Cui C, He F, Zhou Q et al (2006) Studies on optimizing production of wasabi plantlet. Acta Horticult Sin 33:876–878Google Scholar
  4. Deccetti SFC, Soares AM, Paiva R, de Castro EM (2008) Effect of the culture environment on stomatal features, epidermal cells and water loss of micropropagated Annona glabra L. plants. Sci Horticult 117:341–344. doi:10.1016/j.scienta.2008.05.020 (Amsterdam)CrossRefGoogle Scholar
  5. Depree JA, Howard TM, Savage GP (1999) Flavour and pharmaceutical properties of the volatile sulphur compounds of Wasabi (Wasabia japonica). Food Res Int 31:329–337. doi:10.1016/S0963-9969(98)00105-7 CrossRefGoogle Scholar
  6. Fujiwara K, Kozai T, Watanabe I (1987) Fundamental studies on environments in plant tissue culture vessels. (3) Measurements of carbon dioxide gas concentration in closed vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets. J Agric Meteorol 43:21–30. doi:10.2480/agrmet.43.21 CrossRefGoogle Scholar
  7. Grout BWW, Donkin ME (1987) Photosynthetic activity of cauliflower meristem cultures in vitro and at transplanting into soil. Acta Hortic 323–328. doi:10.17660/ActaHortic.1987.212.49
  8. Hazarika BN (2003) Acclimatization of tissue-cultured plants. Curr Sci 85:1704–1712. doi:10.1007/s10529-010-0290-0 Google Scholar
  9. Hung CD, Johnson K, Torpy F (2006) Liquid culture for efficient micropropagation of Wasabia japonica (Miq.) Matsumura. In vitro Cell Dev Biol Plant 42:548–552. doi:10.1079/IVP2006805 CrossRefGoogle Scholar
  10. Iarema L, da Cruz ACF, Saldanha CW et al (2012) Photoautotrophic propagation of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen]. Plant Cell Tissue Organ Cult 110:227–238. doi:10.1007/s11240-012-0145-6 CrossRefGoogle Scholar
  11. Ivanova M, van Staden J (2010) Natural ventilation effectively reduces hyperhydricity in shoot cultures of Aloe polyphylla Schonland ex Pillans. Plant Growth Regul 60:143–150. doi:10.1007/s10725-009-9430-8 CrossRefGoogle Scholar
  12. Jo EA, Tewari RK, Hahn EJ, Paek KY (2009) In vitro sucrose concentration affects growth and acclimatization of Alocasia amazonica plantlets. Plant Cell Tissue Organ Cult 96:307–315. doi:10.1007/s11240-008-9488-4 CrossRefGoogle Scholar
  13. Kim TK (2014) Brassicaceae: Eutrema japonicum. Edible medicinal and non medicinal plants: modified stems, roots, bulbs, Vol 9. Springer, Dordrecht, pp 789–800Google Scholar
  14. Kozai T (2010) Photoautotrophic micropropagation—environmental control for promoting photosynthesis. Propag Ornam Plants 10:188–204Google Scholar
  15. Kozai T, Kubota C (2001) Developing a photoautotrophic micropropagation system for woody plants. J Plant Res 114:525–537. doi:10.1007/PL00014020 CrossRefGoogle Scholar
  16. Kozai T, Fujiwara K, Watanabe I (1986) Fundamental studies on environments in plant tissue culture vessels (2) Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers. J Agric Meteorol 42:119–127CrossRefGoogle Scholar
  17. Kubota C, Kakizaki N, Kozai T et al (2001) Growth and net photosynthetic rate of tomato plantlets during photoautotrophic and photomixotrophic micropropagation. HortScience 36:49–52Google Scholar
  18. Le VQ, Samson G, Desjardins Y (2001) Opposite effects of exogenous sucrose on growth, photosynthesis and carbon metabolism of in vitro plantlets of tomato (L. esculentum Mill.) grown under two levels of irradiances and CO2 concentration. J Plant Physiol 605:599–605. doi:10.1078/0176-1617-00315 Google Scholar
  19. Lian ML, Murthy HN, Paek KY (2002) Culture method and photosynthetic photon flux affect photosynthesis, growth and survival of Limonium “Misty Blue” in vitro. Sci Horticult 95:239–249. doi:10.1016/S0304-4238(02)00039-0 CrossRefGoogle Scholar
  20. Martins JPR, Verdoodt V, Pasqual M, De Proft M (2015) Impacts of photoautotrophic and photomixotrophic conditions on in vitro propagated Billbergia zebrina (Bromeliaceae). Plant Cell Tissue Organ Cult 123:121–132. doi:10.1007/s11240-015-0820-5 CrossRefGoogle Scholar
  21. Mills D, Yanqing Z, Benzioni A (2009) Effect of substrate, medium composition, irradiance and ventilation on jojoba plantlets at the rooting stage of micropropagation. Sci Horticult 121:113–118. doi:10.1016/j.scienta.2009.01.021 CrossRefGoogle Scholar
  22. Mohamed MAH, Alsadon AA (2010) Influence of ventilation and sucrose on growth and leaf anatomy of micropropagated potato plantlets. Sci Horticult 123:295–300. doi:10.1016/j.scienta.2009.09.014 CrossRefGoogle Scholar
  23. Mosaleeyanon K, Cha-Um S, Kirdmanee C (2004) Enhanced growth and photosynthesis of rain tree (Samanea saman Merr.) plantlets in vitro under a CO2-enriched condition with decreased sucrose concentrations in the medium. Sci Horticult 103:51–63. doi:10.1016/j.scienta.2004.02.010 CrossRefGoogle Scholar
  24. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
  25. Nguyen QT, Kozai T (1998) Environmental effects on the growth of plantlets in micropropagation. Environ Control Biol 36:59–75. doi:10.2525/ecb1963.36.59 CrossRefGoogle Scholar
  26. Nguyen QT, Kozai T (2001) Photoautotrophic micropropagation of tropical and subtropical woody plants. In: Morohoshi N, Komamine A (eds) Molecular Breeding of Woody Plants. Elsevier Science B.V., Amsterdams, The Netherlands, pp 335–344Google Scholar
  27. Oh MM, Seo JH, Park JS, Son JE (2012) Physicochemical properties of mixtures of inorganic supporting materials affect growth of potato (Solanum tuberosum L.) plantlets cultured photoautotrophically in a nutrient-circulated micropropagation system. Hortic Environ Biotechnol 53:497–504. doi:10.1007/s13580-012-0043-1 CrossRefGoogle Scholar
  28. Paine CET, Marthews TR, Vogt DR et al (2012) How to fit nonlinear plant growth models and calculate growth rates: An update for ecologists. Methods Ecol Evol 3:245–256. doi:10.1111/j.2041-210X.2011.00155.x CrossRefGoogle Scholar
  29. Palmer J (1990) Germination and growth of wasabi (Wasabia japonica (Miq.) Matsumura). New Zeal J Crop Horticult Sci 18:161–164. doi:10.1080/01140671.1990.10428089 CrossRefGoogle Scholar
  30. Park SY, Moon HK, Murthy HN, Kim YW (2011) Improved growth and acclimatization of somatic embryo-derived Oplopanax elatus plantlets by ventilated photoautotrophic culture. Biol Plant 55:559–562. doi:10.1007/s10535-011-0125-4 CrossRefGoogle Scholar
  31. Roh KS, Choi BY (2004) Sucrose regulates growth and activation of Rubisco in tobacco leaves in vitro. Biotechnol Bioprocess Eng 9:229–235. doi:10.1007/BF02942298 CrossRefGoogle Scholar
  32. Salvin S, Bourke M, Byrne T (eds) (2004) The new crop industries handbook. Rural Industries Research and Development Corporation, CanberraGoogle Scholar
  33. Shin KS, Park SY, Paek KY (2013) Sugar metabolism, photosynthesis, and growth of in vitro plantlets of Doritaenopsis under controlled microenvironmental conditions. In vitro Cell Dev Biol Plant 49:445–454. doi:10.1007/s11627-013-9524-x CrossRefGoogle Scholar
  34. Smith EF, Gribaudo I, Roberts AV, Mottley J (1992) Paclobutrazol and reduced humidity improve resistance to wilting of micropropagated grapevine. HortScience 27:111–113Google Scholar
  35. Soffer H, Burger DW (1988) Effects of dissolved oxygen concentrations in aero-hydroponics on the formation and growth of adventitious roots. J Am Soc Hortic Sci 113:218–221Google Scholar
  36. Sparrow A (2009) Increasing the production of Australian wasabi. Rural Industries Research and Development Corporation, CanberraGoogle Scholar
  37. Sultana T, Savage GP (2008) Wasabi—Japanese Horseradish. Bangladesh J Sci Ind Res 43:433–448. doi:10.3329/bjsir.v43i4.2234 CrossRefGoogle Scholar
  38. Tsay H-S, Lee C-Y, Agrawal DC, Basker S (2006) Influence of ventilation closure, gelling agent and explant type on shoot bud proliferation and hyperhydricity in Scrophularia yoshimurae—A medicinal plant. In vitro Cell Dev Biol Plant 42:445–449. doi:10.1079/IVP2006791 CrossRefGoogle Scholar
  39. Van der Meeren P, De Vleeschauwer D, Debergh P (2001) Determination of oxygen profiles in agar-based gelled in vitro plant tissue culture media. Plant Cell Tissue Organ Cult 65:239–245. doi:10.1023/A:1010698225362 CrossRefGoogle Scholar
  40. Zobayed SMA, Afreen F, Kubota C, Kozai T (2000) Water control and survival of Ipomoea batatas grown photoautotrophically under forced ventilation and photomixotrophically under natural ventilation. Ann Bot 86:603–610. doi:10.1006/anbo.2000.1225 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Department of Environmental Sciences and TechnologyOsaka Prefecture UniversitySakaiJapan

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