Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 122, Issue 3, pp 767–772 | Cite as

Abiotic stresses increase plant regeneration ability of rhizome explants of Curcuma caesia Roxb.

Research Note

Abstract

Curcuma caesia Roxb., commonly called “Black turmeric”, is an important medicinal plant. Normally this plant is micropropagated via rhizome or rhizome bud explant culture. However, the shoot induction from rhizome explants is low and thus, alternate methods could be adopted to induce maximum shoots. In this report, we subjected the rhizome segments to abiotic stresses such as warm water, hot air, cold water and sodium chloride (NaCl) at various time intervals for testing their effect on shoot induction. The best shoot induction medium (control) in terms of percent explants producing shoots (66 %) and number of shoots (11.2 per explant) was Murashige and Skoog medium supplemented with 6-thidiazuron (6.8 µM) and indole-3-butyric acid (1.5 µM). The application of various stresses significantly increased both percent response and number of shoots. Rhizome explants exposed to 1 or 2 h at 30–36 °C warm water significantly improved the response. Optimum response (94 % cultures responding with 22.4 shoots per explants) was observed when rhizomes were exposed at 33 °C for 1 h. In hot air as well as cold water treatments the percent cultures responding and number of shoots showed moderate increase. The increase brought about by 300 and 500 mM NaCl for 1–3 h was significantly higher than control. The rhizomes pretreated with 500 mM NaCl for 3 h showed highest response in contrast to other treatments. Here, 96 % rhizomes responded with an average number of 23.4 shoots per explant. These observations supported the positive role of abiotic stresses in inducing shoots from rhizome segments of C. caesia in cultures.

Keywords

Abiotic stress Curcuma caesia Medicinal plant Rhizome Shoot induction Transplantation 

Abbreviations

BA

6-Benzylaminopurine

IBA

Indole-3-butyric acid

MS

Murashige and Skoog

NAA

Naphthalene acetic acid

NaCl

Sodium chloride

TDZ

Thidiazuron

Supplementary material

11240_2015_795_MOESM1_ESM.doc (680 kb)
Supplementary material 1 (DOC 692 kb)

References

  1. Anonymous (2008) National Medicinal Plants Board, 2008. Agro-techniques if selected medicinal plants, vol 1. The Energy and Resources Institute Press, New Delhi, pp 73–76Google Scholar
  2. Balachandran SM, Bhat SR, Chandal KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep 8:521–524PubMedCrossRefGoogle Scholar
  3. Banerjee A, Nigam SS (1976) Antifungal activity of the essential oil of Curcuma caesia Roxb. Indian J Med Res 64:1318–1321PubMedGoogle Scholar
  4. Bharalee R, Das A, Kalita MC (2005) In vitro clonal propagation of Curcuma caesia Roxb and Curcuma zedoaria Rosc from rhizome bud explants. J Plant Biochem Biotech 14:61–63CrossRefGoogle Scholar
  5. Bohnert HJ, Nelson DE, Jonsen RG (1995) Adaptations to environmental stresses. Plant Cell 7:1099–1111PubMedCentralPubMedCrossRefGoogle Scholar
  6. Duncan DB (1995) Multiple range and multiple F tests. Biometrics 11:1–42CrossRefGoogle Scholar
  7. Greenway H, Munns R (1980) Mechanisms of salt tolerance in nonhalophytes. Ann Rev Plant Physiol 31:149–190CrossRefGoogle Scholar
  8. Ishimine Y, Hossain MA, Motomura K, Akamine H, Hirayama T (2004) Effects of planting date on emergence, growth and yield of turmeric (Curcuma longa L.) in Okinawa prefecture, Southern Japan. Japanese J Trop Agric 48:10–16Google Scholar
  9. Jose S, Thomas TD (2014) Comparative phytochemical and antibacterial studies of two indigenous medicinal plants Curcuma caesia Roxb. and Curcuma aeruginosa Roxb. Int J Green Pharm 8:65–71CrossRefGoogle Scholar
  10. Karmakar I, Dolai N, Saha P, Sarkar N, Bala A, Haldar PK (2011) Scavenging activity of Curcuma caesia rhizome against reactive oxygen and nitrogen species. Orient Pharm Exp Med 11:221–228CrossRefGoogle Scholar
  11. Karmakar I, Dolai N, Kumar RBS, Kar B, Roy SN, Haldar PK (2013) Antitumor activity and antioxidant property of Curcuma caesia against Ehrlich’s ascites carcinoma bearing mice. Pharm Biol 51:753–759PubMedCrossRefGoogle Scholar
  12. Loc NH, Duc DT, Kwon TH, Yang MS (2005) Micropropagation of zedoary (Curcuma zedoaria Roscoe): a valuable medicinal plant. Plant Cell Tissue Organ Cult 81:119–122CrossRefGoogle Scholar
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  14. Paliwal P, Pancholi SS, Patel RK (2011) Pharmacognostic parameters for evaluation of the rhizomes of Curcuma caesia. J Adv Pharm Technol Res 2:56–61PubMedCentralPubMedCrossRefGoogle Scholar
  15. Pedersen C, Hansen CW, Brandt IL, Kristiansen K (1996) Alstroemeria plantlets can be induced to flowering by cold treatment during in vitro culture. Sci Hortic 66:217–228CrossRefGoogle Scholar
  16. Prakash S, Elangomathavan R, Seshadri S, Kathiravan K, Ignacimuthu S (2004) Efficient regeneration of Curcuma amada Roxb. plantlets from rhizome and leaf sheath explants. Plant Cell Tissue Organ Cult 78:159–165CrossRefGoogle Scholar
  17. Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2005) Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium. Plant Cell Tissue Organ Cult 80:347–351CrossRefGoogle Scholar
  18. Puijalon S, Piola F, Bornette G (2008) Abiotic stresses increase plant regeneration ability. Evol Ecol 22:493–506CrossRefGoogle Scholar
  19. Pumisutapon P, Visser RGF, Jan de Klerk G (2012) Moderate abiotic stresses increase rhizome growth and outgrowth of axillary buds in Alstroemeria cultured in vitro. Plant Cell Tissue Organ Cult 110:395–400CrossRefGoogle Scholar
  20. Rai MK, Kalia RK, Singh R, Gangola MP, Dhawan AK (2011) Developing stress tolerant plants through in vitro selection – An overview of the recent progress. Env Exp Bot 71:89–98Google Scholar
  21. Ravindran PN, Nirmal Babu K, Shiva KN (2007) Botany and crop improvement of turmeric. In: Ravindran PN, Nirmal Babu K, Sivaraman K (eds) Turmeric: The Genus Curcuma. CRC press, London, pp 15–70Google Scholar
  22. Sarangthem K, Haokip MJ (2010) Bioactive components in Curcuma caesia Roxb. grown in Manipur. Bioscan 5:113–115Google Scholar
  23. Sasikumar B (2005) Genetic resources of Curcuma: diversity, characterization and utilization. Plant Genet Resour 3:230–251CrossRefGoogle Scholar
  24. Shahinozzaman M, Ferdous MM, Faruq M, Azad MAK, Amin MN (2013) Micropropagation of black turmeric (Curcuma caesia Roxb.) through in vitro culture of rhizome bud Explants. J Cent Eur Agric 14:110–115CrossRefGoogle Scholar
  25. Shirgurkar MV, John CK, Nadgauda RS (2001) Factors affecting in vitro microrhizome production in turmeric. Plant Cell Tissue Organ Cult 64:5–11CrossRefGoogle Scholar
  26. Siddique AB, Ara I, Shahinul Islam SM, Tuteja N (2014) Effect of air desiccation and salt stress factors on in vitro regeneration of rice. Plant Signal Behav 9:12CrossRefGoogle Scholar
  27. Singh V, Jain AP (2003) Ethnobotany and medicinal plants of India and Nepal, vol II. Scientific Publishers, Jodhpur, pp 562–563Google Scholar
  28. Sivaraman K (2007) Agronomy of Turmeric. In: Ravindran PN, Nirmal Babu K, Sivaraman K (eds) Turmeric: The Genus Curcuma. CRC press, London, pp 129–153Google Scholar
  29. Thomas TD (2003) Thidiazuron induced multiple shoot induction and plant regeneration from cotyledonary explants of mulberry. Biol Plant 46:529–533CrossRefGoogle Scholar
  30. Yancey PH, Clark ME, Hand SC, Bowlis RD, Somero GN (1982) Living with water stress: evolution of osmolyte system. Science 217:1214–1222PubMedCrossRefGoogle Scholar
  31. Zhang S, Liua N, Shenga A, Mab G, Wu G (2011) Direct and callus-mediated regeneration of Curcuma soloensis Valeton (Zingiberaceae) and ex vitro performance of regenerated plants. Sci Hortic 130:899–905CrossRefGoogle Scholar
  32. Zhang ZB (2000) Discussion about studies of plant stress resistance to environment. Acta Agric Boreali-occidentalis Sin 9:112–116Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Research and Development CenterBharathiar UniversityCoimbatoreIndia
  2. 2.Postgraduate and Research Department of BotanySt. Thomas College, PalaiKottayamIndia

Personalised recommendations