Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 121, Issue 3, pp 741–760 | Cite as

In vitro shoot organogenesis and comparative analysis of endogenous phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects of genotype, explant type and applied cytokinins

  • Tatjana Ċosiċ
  • Václav Motyka
  • Martin Raspor
  • Jelena Savić
  • Aleksandar Cingel
  • Branka Vinterhalter
  • Dragan Vinterhalter
  • Alena Trávníčková
  • Petre I. Dobrev
  • Borut Bohanec
  • Slavica Ninković
Original Paper

Abstract

Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple (VP) and Vienna White (VW) were tested for their ability of de novo organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact seedlings were cultivated on Murashige and Skoog (MS) media supplemented with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans- or cis-zeatin. All tested cytokinins, including cis-zeatin, induced shoot regeneration from hypocotyl explants and intact seedlings, with seedlings being most successful for regeneration efficiency and viability of regenerated shoots in both cultivars. The highest frequency of shoot regeneration was achieved on MS with BA (60 %) or TDZ (50 %) for VP; and with BA (50 %), TDZ (47.5 %) or transZ (37.5 %) for VW. Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA) contents in both hypocotyl explants and seedlings with regenerated shoots (HRSs and SRSs) suggested that the observed differences in organogenic response between these two types of explants were related to their cytokinin and IAA contents. HRSs generally exhibited elevated amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive cytokinins ratio. Shoots regenerated from seedlings were further successfully multiplicated on a medium supplemented with BA (0.5 mg L−1). The rooting potential of multiplicated shoots was tested on media supplemented with 2 or 4 mg L−1 indole-3-butyric acid (IBA), with the higher concentration of IBA leading to more efficient rooting. Rooted plantlets were successfully planted into soil and flow cytometric analysis did not reveal ploidy variations, indicating that the described protocol is fast and efficient for kohlrabi regeneration.

Keywords

Kohlrabi Cytokinin Auxin Hypocotyl Seedling De novo shoot organogenesis 

Abbreviations

The system of CK abbreviations was adopted and modified according to Kamínek et al. (2003).

BA

N6-benzyladenine

cisZ

cis-Zeatin

cisZR

cis-Zeatin-9-riboside

cisZ7G

cis-Zeatin-7-glucoside

cisZ9G

cis-Zeatin-9-glucoside

cisZOG

cis-Zeatin-O-glucoside

cisZROG

cis-Zeatin-9-riboside-O-glucoside

cisZRMP

cis-Zeatin-9-riboside-5′-monophosphate

CK

Cytokinin

DNSO

De novo shoot organogenesis

DHZ

Dihydrozeatin

DHZR

Dihydrozeatin-9-riboside

DHZ7G

Dihydrozeatin-7-glucoside

DHZ9G

Dihydrozeatin-9-glucoside

DHZOG

Dihydrozeatin-O-glucoside

DHZROG

Dihydrozeatin-9-riboside-O-glucoside

DHZRMP

Dihydrozeatin-9-riboside-5′-monophosphate

HRS

Hypocotyl with regenerated shoots

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

iP

N6-(∆2-isopentenyl)adenine

iPR

N6-(∆2-isopentenyl)adenosine

iP7G

N6-(∆2-isopentenyl)adenine-7-glucoside

iP9G

N6-(∆2-isopentenyl)adenine-9-glucoside

iPRMP

N6-(∆2-isopentenyl)adenosine -5′-monophosphate

PGR

Plant growth regulator

SRS

Seedling with regenerated shoots

TDZ

Thidiazuron

transZ

trans-Zeatin

transZR

trans-Zeatin-9-riboside

transZ7G

trans-Zeatin-7-glucoside

transZ9G

trans-Zeatin-9-glucoside

transZOG

trans-Zeatin-O-glucoside

transZROG

trans-Zeatin-9-riboside-O-glucoside

transZRMP

trans-Zeatin-9-riboside-5′-monophosphate

VP

Vienna Purple cultivar

VW

Vienna White cultivar

Notes

Acknowledgments

This work was supported by the Ministry of Education, Science and Technological Development of Serbia (No. 173015) and the Czech Science Foundation (P506/11/0774).

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Tatjana Ċosiċ
    • 1
  • Václav Motyka
    • 2
  • Martin Raspor
    • 1
  • Jelena Savić
    • 1
  • Aleksandar Cingel
    • 1
  • Branka Vinterhalter
    • 1
  • Dragan Vinterhalter
    • 1
  • Alena Trávníčková
    • 2
  • Petre I. Dobrev
    • 2
  • Borut Bohanec
    • 3
  • Slavica Ninković
    • 1
  1. 1.Institute for Biological Research “Siniša Stanković”University of BelgradeBelgradeSerbia
  2. 2.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPrague 6Czech Republic
  3. 3.Biotechnical FacultyUniversity of LjubljanaLjubljanaSlovenia

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