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Interaction of rhizobacterial strains for growth improvement of Crocus sativus L. under tissue culture conditions

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Abstract

The co-cultivation of tissue explants with beneficial microbes induces numerous developmental and metabolic alterations in the resulting plantlets conferring enhanced tolerance to abiotic and biotic stresses. In the present study we have co-cultivated plant growth promoting rhizobacteria (PGPR) exhibiting multiple plant growth promoting activities with the in vitro raised saffron cormlets for evaluating various morphogenetic responses like proliferation, germination and weight increment of cormlets. The results obtained indicate the significant effect of Pseudomonas sp., Bacillus subtilis and Pantoea sp. in weight increment of cormlets. Proliferation of cormlets was also significantly improved with Pantoea sp. + Bacillus subtilus + Pseudomonas sp. on MS liquid medium. Similarly, the co-cultivation with Acinetobacter haemolyticus, Accintobacter lwoffii and Pantoea sp. resulted in 100 % germination of cormlets. The root system of cormlets was found denser and thicker than the control cormlets. However, rhizobacterial cormlets exhibited lower values of root length than non-treated cormlets. This study represents earliest report across the globe with suitable and reproducible protocol for corm development through PGPRs.

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Fig. 1

Abbreviations

BAP:

Benzyl aminopurine

NAA:

Naphthalene acetic acid

IAA:

Indole acetic acid

PGP:

Plant growth promoting

PGPR:

Plant growth promoting rhizobacteria

PSB:

Phosphate solubilizing bacteria

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Acknowledgments

This study was supported by DBT, GoI, New Delhi funded project vide letter no: BT/PR5525/PVD/16/980/2012 Dtd.20/11/2012, the assistance of which is highly acknowledged. The authors are thankful to department of Microbiology SKIMS Soura Srinagar and Dr. Qadri’s Lab, Karanagar, J&K for helping in identifying rhizobacterial strains.

Conflict of interest

The authors hereby declare no conflict of interest

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Correspondence to Javid A. Parray.

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Parray, J.A., Kamili, A.N., Reshi, Z.A. et al. Interaction of rhizobacterial strains for growth improvement of Crocus sativus L. under tissue culture conditions. Plant Cell Tiss Organ Cult 121, 325–334 (2015). https://doi.org/10.1007/s11240-014-0703-1

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Keywords

  • Co-cultivation
  • In vitro culture
  • Rhizobacteria
  • Saffron