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Transformation of sundew: pitfalls and promises

Abstract

Functional studies and exploitation of pharmacological potential of sundews (Droseraceae) require efficient and reliable transformation protocol. However, the routine applicability of available procedures appears limited and transformation of sundew remains a difficult task. Here we report on in-depth evaluation of the previously published method and bring some modifications. We suggest addition of glutathione in cultivation media and protection from light for suppression of necrosis. Further we show that sundew leaf extracts exert antimicrobial activity, therefore we recommend selection of tolerant Agrobacterium strain. At the same time we conclude that A. rhizogenes is not suitable for sundew transformation since produces only albino plants. To support regeneration of transformed shoots we propose to use GFP as a vital marker for early identification and removal of escapes and chimeras. The modified protocol we provide enables a routine and reliable selection of stably transformed sundew plants.

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Acknowledgments

This work was supported by the Slovak Research and Development Agency under the Contract No. APVV-0140-10, COST FA 1006 and VEGA Project No. 2/0090/14. We would like to thank to Prof. J. Šamaj, Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Cell Biology, Faculty of Science Palacký University, Olomouc, Czech Republic, for providing the construct used in this work.

Conflict of interest

The authors declare that they have no conflict of interest.

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Correspondence to Renáta Švubová.

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Blehová, A., Švubová, R., Lukačová, Z. et al. Transformation of sundew: pitfalls and promises. Plant Cell Tiss Organ Cult 120, 681–687 (2015). https://doi.org/10.1007/s11240-014-0635-9

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Keywords

  • Agrobacterium
  • Antioxidants
  • Drosera rotundifolia
  • Escapes
  • Green fluorescent protein
  • Chimera
  • Necrosis