Chromosome elimination and introgression following somatic hybridization between bread wheat and other grass species
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The timing and influencing factor of donor chromosome elimination and introgression in somatic hybrids involving bread wheat (Triticum aestivum L.) was investigated by sampling fusion products made between bread wheat and four different donor species. Genomic in situ hybridization experiments showed that both events in the main occurred within 12–15 days of the fusion. Over 70 % of the elimination events had taken place before the first mitotic interphase. Pre-treating the donor protoplasts by either γ or UV irradiation showed that the severity of exposure affected the likelihood of chromosome elimination and introgression. More donor chromosome elimination and less introgression occurred when donors were phylogenetically more distant, such as annual meadow grass (Poaannua) or maize (Zea mays), than phylogenetically closer donors such as tall wheatgrass [Agropyron elongatum (Thinopyrum elongatum)] or Haynaldia villosa. The interaction effect between donor species and irradiation time significantly affected the chromatin behaviors during somatic hybridization. Besides, the polyploid A. elongatum behaved differently from diploid H. villosa, even though these two species are phylogenetically equidistant from wheat.
KeywordsTriticum aestivum L. Somatic hybridization Chromosome elimination Chromosome introgression Micronuclei Radiation dosage Phylogenetical relationship
This work was supported by the funds of the Natural Science Foundation of China (No. 31270385 and 31201499), the Natural Science Foundation of Zhejiang Province, China (Y3110313) and the National Key Technology R&D Program of China (2012BAD27B01).
- Ding L, Zhao ZG, Ge XH, Li ZY (2014) Different timing and spatial separation of parental chromosomes in intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus. Genet Mol Res 13:1618–2611Google Scholar
- Dong YC (2000) Gene pool of wheat. J Triticeae crops 20(3):78–81 (in Chinese)Google Scholar
- Xia GM, Wang H, Chen HM (1996) Plant regeneration from intergeneric asymmetric somatic hybridization between wheat (Triticumn aestivum L.) and Russian wild rye (Psathyrostachys juncea (Fisch) Neweski) and couch grass (Agropyron elongatumn Host Neviski). Chin Sci Bull 41:1382–1386Google Scholar
- Zhou AF, Xia GM, Chen HM (1996) Asymmetric somatic hybridization between Triticum aestivum and Haynaldia villosa Schur. Sci China 39:617–626Google Scholar