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In vitro propagation of a poisonous plant Oxytropis glabra (Lam.) DC

Abstract

An efficient micropropagation system for Oxytropis glabra, a poisonous grass species, had been developed. Shoot organogenesis occurred from O. glabra leaf explants on medium with appropriate supplements of plant growth regulators. 85.6 % of shoot regeneration frequency and 8.2 shoots per leaf explant were achieved when cultured on a medium containing 10 μM 6-benzylaminopurine and 1.0 μM naphthaleneacetic acid. Histological studies of these explants confirmed their organogenic nature, by demonstrating the formation of shoots. Shoot organogenesis was further improved when the leaf explants were pre-incubated at 4 °C by a 7-day pretreatment, and shoot regeneration frequency of 88.4 % was recorded with 12.6 shoots per leaf explant. Up to 86.8 % of the regenerated roots formed complete plantlets on a medium containing 1.0 μM indole-3-butyric acid within 35 days. The micropropagated plants were similar to wild plants. Swainsonine was presented in wild O. glabra, but were not detected in O. glabra in vitro culture.

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Abbreviations

BAP 6:

Benzylaminopurine

2, 4-D:

2, 4-dichlorophenoxyacetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog

NAA:

Naphthaleneacetic acid

TDZ:

Thidiazuron

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Acknowledgments

The work was supported by Special Fund for Agro-scientific Research in the Public Interest of China (No. 201203062), and research fund from Northwest University (No. PR12082 and No. NH13030).

Author information

Correspondence to Yahui Wei.

Additional information

Wei He and Bin Guo have contributed equally to this work.

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He, W., Guo, B., Fan, P. et al. In vitro propagation of a poisonous plant Oxytropis glabra (Lam.) DC. Plant Cell Tiss Organ Cult 120, 49–55 (2015). https://doi.org/10.1007/s11240-014-0577-2

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Keywords

  • Oxytropis glabra
  • Micropropagation
  • Poisonous plant
  • Phytochemicals
  • Swainsonine