Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 119, Issue 3, pp 553–563 | Cite as

A poplar R2R3-MYB transcription factor, PtrMYB152, is involved in regulation of lignin biosynthesis during secondary cell wall formation

  • Chaofeng Li
  • Xianqiang Wang
  • Wanxiang Lu
  • Rui Liu
  • Qiaoyan Tian
  • Yiming Sun
  • Keming Luo
Original Paper
First Online:
Received:
Accepted:

Abstract

Wood is the most abundant biomass in perennial woody plants. Extensive studies have shown that R2R3-MYB transcription factors are involved in regulation of lignin biosynthesis during secondary cell wall formation in many plant species, such as Arabidopsis, rice, maize and poplar. In this study, a MYB transcription factor, named PtrMYB152, was isolated from Populus trichocarpa and encoded a protein of 321 amino acids that contained a conserved R2R3-MYB domain. Phylogenetic analysis revealed that PtrMYB152 shares high sequence homology with other known plant MYBs associated with secondary wall formation. PtrMYB152 is specifically expressed in secondary wall-forming cells in poplar. Histochemical localizations of GUS expression in the transgenic Arabidopsis plants showed that PtrMYB152 is predominantly expressed in vascular tissues. Overexpression of PtrMYB152 resulted in specific activation of lignin biosynthetic genes, and caused ectopic deposition of lignin in stem and petiole of transgenic plants. These data indicated that PtrMYB152 is a specific transcriptional activator of lignin biosynthesis during wood formation of poplar.

Keywords

Poplar R2R3-MYB Transcription factors Lignin Secondary cell walls 
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Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (31370672, 31171620), the National Key Project for Research on Transgenic Plant (2011ZX08010-003), 100 Talents Programme of The Chinese Academy of Sciences, the Natural Science Foundation Project of CQ CSTC (CSTC2013JJB8007), the program for New Century Excellent Talents in University (NCET-11-0700), the Fundamental Research Funds for the Central Universities (XDJK2012c088, XDJK2014a005) and the Research Fund for the Doctoral Program of Higher Education (20110182110004).

Supplementary material

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Chaofeng Li
    • 1
  • Xianqiang Wang
    • 1
  • Wanxiang Lu
    • 1
  • Rui Liu
    • 1
  • Qiaoyan Tian
    • 1
  • Yiming Sun
    • 1
  • Keming Luo
    • 1
    • 2
  1. 1.Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life SciencesSouthwest UniversityChongqingChina
  2. 2.Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningChina

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