Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 119, Issue 3, pp 501–509 | Cite as

Effect of a novel aromatic cytokinin derivative on phytochemical levels and antioxidant potential in greenhouse grown Merwilla plumbea

  • Adeyemi O. Aremu
  • Mack Moyo
  • Stephen O. Amoo
  • Jiří Gruz
  • Michaela Šubrtová
  • Lucie Plíhalová
  • Karel Doležal
  • Johannes Van Staden
Original Paper

Abstract

In an attempt to elucidate the carry-over effect of cytokinins (CKs) on phytochemical and antioxidant activity of acclimatized plants, tissue culture-derived Merwilla plumbea supplemented with three CK types at four (0.25, 0.5, 1 and 2 µM) concentrations were grown for 6 months ex vitro. Phenolic acids including the hydroxybenzoic and hydroxycinnamic acid derivatives in M. plumbea were quantified using ultra performance liquid chromatography while the antioxidant activity was evaluated using oxygen radical absorbance capacity (ORAC). Different concentrations of gallic acid, protocatechuic acid, p-hydroxybenzoic acid and salicylic acid were observed with all the treatments with the exception of non-treated plants. Most phytochemicals (for example, gallic acid, ferulic acid protocatechuic acid and caffeic acid) were highest in plants obtained from 0.25 µM meta-topolin riboside (mTR). Likewise, plants derived from 2 µM mTR had the highest ORAC (684 µmol g−1 trolox equivalents) activity. Bearing in mind that therapeutic effects of medicinal plants are often associated to their phytochemical content, the current results are an indication on how the intricate in vitro environment (CK type and concentration in this case) affects the growth and general physiology of micropropagated plants especially after acclimatization.

Keywords

Antioxidant Conservation Phenolics Phytohormones Topolins Secondary metabolites 

Abbreviations

AAPH

2,2′-Azobis(2-methylpropionamidine) dihydrochloride

ANOVA

Analysis of variance

CK

Cytokinin

DMRT

Duncan‘s multiple range test

DW

Dry weight

GAE

Gallic acid equivalents

MS

Murashige and Skoog medium

mTR

meta-Topolin riboside

mTTHP

6-(3-Hydroxybenzylamino)-9-tetrahydropyran-2-ylpurine

ORAC

Oxygen radical absorbance capacity

PPF

Photosynthetic photon flux

TDZ

Thidiazuron

TE

Trolox equivalents

UPLC

Ultra performance liquid chromatography

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Adeyemi O. Aremu
    • 1
  • Mack Moyo
    • 1
  • Stephen O. Amoo
    • 1
  • Jiří Gruz
    • 2
  • Michaela Šubrtová
    • 2
  • Lucie Plíhalová
    • 2
  • Karel Doležal
    • 2
  • Johannes Van Staden
    • 1
  1. 1.Research Centre for Plant Growth and Development, School of Life SciencesUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa
  2. 2.Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of SciencePalacký University and Institute of Experimental Botany, Academy of Sciences of Czech RepublicOlomouc-HoliceCzech Republic

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