Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 119, Issue 3, pp 469–477 | Cite as

Enhanced plumbagin accumulation in embryogenic cell suspension cultures of Plumbago rosea L. following elicitation

  • P. K. Silja
  • G. P. Gisha
  • K. Satheeshkumar
Original Paper


This study focused on enhancing the production of plumbagin, an anticancer compound, in embryogenic cell suspension cultures of Plumbago rosea. Elicitation techniques have been reported to enhance plumbagin production. Cell suspension cultures raised from embryogenic calli induced from in vitro leaf explants were exposed to different concentrations of jasmonic acid, yeast extract and different auxin combinations. Influence of these on cell growth, biomass and plumbagin production was studied. To our knowledge this is the first report on elicitation of embryogenic cell suspension cultures of P. rosea for enhanced plumbagin production. Elicitor treated suspension cultures exhibited decreased culture viability and increased plumbagin synthesis. A maximum of 5.59-fold enhancement of plumbagin production was observed in cultures added with 1 mg L−1 naphthalene acetic acid after 6 days of incubation. Viability of cultures decreased with increased concentration of elicitors and prolonged incubation period. Application of elicitors in cell suspension cultures induces defense related responses which lead to increased secondary metabolite production for making the cells adapt to the situation. If the stressed condition persists or is in intolerable level this will eventually lead to programmed cell death and loss of culture viability.


Elicitor Jasmonic acid Yeast extract Hypersensitive response Stress induction Embryogenic suspension 



The authors thank the Director of the Institute for providing facilities and Council for Scientific and Industrial Research (CSIR), Government of India for financial support as Ph.D fellowship.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Biotechnology and Bioinformatics DivisionJawaharlal Nehru Tropical Botanic Garden and Research InstituteTrivandrumIndia

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