Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 118, Issue 1, pp 169–177 | Cite as

Effect of plant growth regulators and elicitors on rhinacanthin accumulation in hairy root cultures of Rhinacanthusnasutus (L.) Kurz

Original Paper

Abstract

Several biologically active secondary metabolites like anthraquinones, sterols, triterpenes, flavonoids and naphthoquinones are present in Rhinacanthus nasutus. Naphthoquinones are important group of compounds generally known as rhinacanthin (RC) consists of 15 derivatives named RC A–D and G–Q of which RC-C, RC-D and RC-N have various medicinal properties. The individual role of two auxins i.e. indole-3-butyric acid (IBA) and α naphthalene acetic acid (NAA) and two elicitors i.e. methyl jasmonate (MJ) and salicylic acid (SA) in Murashige and Skoog medium on hairy root growth and RC (RC-C, RC-D and RC-N) accumulation was investigated in the present study. Time course study revealed that IBA and NAA at 2.5 μM showed maximum fresh weight (FW) and dry weight (DW) 4 weeks after culture. However, RC production was maximum after 6 weeks of culture on both media. A concentration-dependent response was observed when various concentrations of MJ (2.0, 5.0, 10 and 15 μM) and SA (10, 50, 100 and 150 μM) were supplemented in the medium. On MJ and SA media the FW and DW decreased as the concentration of elicitors increased. However, this decrease was more severe in MJ treated cultures. All the MJ and SA treated cultures showed significantly higher amount of RC-C, RC-D and RC-N in hairy roots harvested 7 days after elicitation as compared to control. Of the two elicitors, MJ was more efficient in inducing RC accumulation than SA. The highest RC content (6.3 mg/g DW RC-C; 1.1 mg/g DW RC-D and 0.61 mg/g DW RC-N) was observed after treatment with 10 μM MJ which was about 1.7-, 2.5- and 3.5-fold higher RC-C, RC-D and RC-N respectively than the control.

Keywords

Auxins Elicitors Hairy root Rhinacanthin Rhinacanthus nasutus Time course 

Abbreviation

DW

Dry weight

FW

Fresh weight

HPLC

High performance liquid chromatography

IBA

Indole-3-butyric acid

MJ

Methyl jasmonate

MS

Murashige and Skoog medium

NAA

α Naphthalene acetic acid

PCR

Polymerase chain reaction

RC

Rhinacanthin

SA

Salicylic acid

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Post Graduate and Research Department of BotanySt. Thomas CollegePala, KottayamIndia
  2. 2.Department of Pharmaceutical Biology and BotanyMedical University in WroclawWroclawPoland

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