Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 116, Issue 1, pp 17–26 | Cite as

Physiological effects of a novel aromatic cytokinin analogue in micropropagated Aloe arborescens and Harpagophytum procumbens

  • Stephen O. Amoo
  • Adeyemi O. Aremu
  • Mack Moyo
  • Lucie Szüčová
  • Karel Doležal
  • Johannes Van Staden
Original Paper

Abstract

Globally, there is a continuous effort geared toward improving micropropagation protocols with much emphasis on the type of cytokinins (CKs). We investigated the effect of meta-topolin tetrahydropyran-2-yl [mTTHP—a novel derivative of the aromatic cytokinin meta-topolin (mT)] on shoot proliferation, photosynthetic pigment content, phytochemicals and antioxidant activity of two widely used medicinal plants, Aloe arborescens and Harpagophytum procumbens. In terms of shoot proliferation, besides mTTHP and mT (at equimolar level) showing comparable effects, both topolins were significantly better than the control and benzyladenine riboside (BAR) in micropropagated A. arborescens. In H. procumbens, mT-treated cultures were the most responsive treatment at 2.5 μM when compared to the control. At 5.0 μM concentration, mTTHP and meta-topolin riboside (mTR) demonstrated a comparable effect on shoot proliferation. To a certain extent (particularly at low concentrations), mTTHP had a better rooting stimulatory effect when compared to the other CKs in both investigated species. In micropropagated A. arborescens, 2.5 μM mTTHP showed the best antioxidant activity (ferric reducing power) which was significantly higher than the control and all BAR-treated plants. Although 2.5 μM mTTHP exhibited a significant antioxidant activity (in β-carotene based assay) when compared to mTR at equimolar level in H. procumbens, it was not significantly different from the treatment with 2.5 μM mT and the control. As evidenced in the current study, the efficacy of the novel cytokinin may be species-specific and beneficial for few physiological parameters. It is conceivable that mTTHP is another viable alternative topolin with the extra advantage of inducing rooting at a low concentration.

Keywords

Antioxidant Chlorophyll Medicinal plants Secondary metabolites Topolins 

Abbreviations

ANOVA

Analysis of variance

BA

N6-Benzyladenine

BAR

Benzyladenine riboside

BHT

Butylated hydroxytoluene

CE

Catechin equivalents

CKs

Cytokinins

CKX

Cytokinin oxidase/dehydrogenase

DPPH

2,2-Diphenyl-1-picryl hydrazyl

DW

Dry weight

GAE

Gallic acid equivalents

HE

Harpagoside equivalents

IBA

Indole butyric acid

MemTTHP

meta-Methoxytopolin 9-tetrahydropyran-2-yl or 6-(3-methoxybenzylamino-9-tetrahydropyran-2ylpurine

MS

Murashige and Skoog

mT

meta-Topolin

mTR

meta-Topolin riboside

mTTHP

meta-Topolin tetrahydropyran-2-yl or 6-(3-hydroxybenzylamino)-9-tetrahydropyran-2ylpurine

PGR

Plant growth regulator

PGRs

Plant growth regulators

PTC

Plant tissue culture

THP

Tetrahydropyranyl

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Stephen O. Amoo
    • 1
  • Adeyemi O. Aremu
    • 1
  • Mack Moyo
    • 1
  • Lucie Szüčová
    • 2
    • 3
  • Karel Doležal
    • 2
    • 3
  • Johannes Van Staden
    • 1
  1. 1.Research Centre for Plant Growth and Development, School of Life SciencesUniversity of KwaZulu-Natal, PietermaritzburgScottsvilleSouth Africa
  2. 2.Laboratory of Growth RegulatorsPalackỳ University and Institute of Experimental Botany AS CROlomoucCzech Republic
  3. 3.Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of SciencePalackỳ UniversityOlomoucCzech Republic

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