Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 115, Issue 3, pp 385–393 | Cite as

Repetitive somatic embryogenesis from leaves of the medicinal plant Petiveria alliacea L.

  • L. Cantelmo
  • B. O. Soares
  • L. P. Rocha
  • J. A. Pettinelli
  • C. H. Callado
  • E. Mansur
  • A. Castellar
  • R. F. Gagliardi
Original Paper
First Online:
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Accepted:

Abstract

Leaf segments from in vitro-grown shoot cultures of Petiveria alliacea were incubated on Murashige and Skoog (MS) medium supplemented with different concentrations of zeatin, thidiazuron, 2,4-dichlorophenoxyacetic acid (2,4-D) or picloram (PIC). Direct somatic embryogenesis was induced in response to all tested concentrations of 2,4-D and PIC. Primary somatic embryos displayed highly repetitive embryogenesis, both on the induction medium and in liquid hormone-free MS medium. Plantlets were obtained from these secondary embryos at an estimated frequency of 5 %, after 180 days of culture on half-strength MS medium gelled with 0.2 % Phytagel. Simultaneous development of friable non-embryogenic callus was also observed on media containing PIC or 2,4-D at different concentrations. Cell suspension cultures initiated from these callus tissues did not show an increase in biomass. The embryogenic portions formed at the surface of the explants in response to 20.0 μM PIC were inoculated in hormone-free full-or half-strength liquid MS medium (MS0) and showed high rates of secondary embryogenesis, resulting in the production of a mean of 35 embryos for each embryo inoculated at the culture initiation. Embryos that started the conversion process in the liquid MS0 medium originated whole plants at a frequency of 100 % when transferred to MS0 medium solidified with 0.7 % agar. Acclimatization was achieved in 90 % of the converted plantlets, with the production of phenotypically normal plants. This system is potentially useful for the micropropagation of this species, as well as for the production of substances with pharmacological interest, such as dibenzyl trisulfide.

Keywords

Phytolaccacea Embryogenic cell suspension Direct somatic embryogenesis Callus Dibenzyl trisulfide Picloram 
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Notes

Acknowledgments

This work was sponsored by the Carlos Chagas Filho Foundation for Research Support of Rio de Janeiro (FAPERJ), the National Council for Scientific and Technological Development (CNPq/Brazil), and the Foundation for the Coordination of Improvement of Higher Education Personnel (CAPES).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • L. Cantelmo
    • 1
  • B. O. Soares
    • 1
  • L. P. Rocha
    • 1
  • J. A. Pettinelli
    • 1
  • C. H. Callado
    • 2
  • E. Mansur
    • 1
  • A. Castellar
    • 3
  • R. F. Gagliardi
    • 1
  1. 1.Núcleo de Biotecnologia VegetalUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Laboratório de Anatomia VegetalUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Laboratório de Produtos Naturais e AlimentosUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil

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