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Production of salidroside and tyrosol in cell suspension cultures of Rhodiola crenulata

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Abstract

Salidroside and its aglycone tyrosol are important compounds found in Rhodiola plants. In this study, callus derived from Rhodiola crenulata was induced and grown when explants were incubated on a Murashige and Skoog (MS) medium containing various concentrations of 6-benzyaldenine (BA), naphthalene acetic acid (NAA) and thidiazuron (TDZ). Callus was easily initiated from juvenile leaves in half strength MS medium supplemented with 0.5 mg/L BA and 3.0 mg/L NAA, while full strength MS containing 0.5 mg/L TDZ and 0.5 mg/L NAA was the best for callus subculture and subsequent cell suspension culture. The activities of l-phenylalanine ammonia lyase (PAL) and β-d-glucosidase, two key enzymes in salidroside synthesis, increased at first and subsequently decreased in cell suspension cultures. The salidroside and tyrosol levels in the cell suspension cultures were determined using high-performance liquid chromatography. High levels of salidroside and tyrosol were detected in cell suspension cultures of R. crenulata extracted with 75 % methanol, demonstrating that the biotechnological production of these compounds using plant cell suspension cultures derived from R. crenulata may be an attractive alternative to harvest-based production.

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Acknowledgments

The study was financially supported by the Fundamental Research Funds for the Central Universities, China (YX2012-02) and the Ph.D. Programs Foundation of Ministry of Education of China (20120014110006).

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Correspondence to Yujun Liu or Chao Ma.

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Shi, L., Wang, C., Zhou, X. et al. Production of salidroside and tyrosol in cell suspension cultures of Rhodiola crenulata . Plant Cell Tiss Organ Cult 114, 295–303 (2013). https://doi.org/10.1007/s11240-013-0325-z

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Keywords

  • Rhodiola crenulata
  • Cell suspension cultures
  • Salidroside
  • Tyrosol
  • l-phenylalanine ammonia lyase
  • β-d-glucosidase